手机验证
上海联迈生物工程有限公司
大量
LM-1630R
多克隆
Rabbit
1年
CEBP-alpha
转录调节因子C/EBP α 抗体
Rabbit
Human, Mouse, Rat, Dog, Pig, Cow, Rabbit,
KLH conjugated synthetic peptide derived from human CEBP-alpha:251-358/358
IgG
Lyophilized or Liquid
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/test IF=1:100-500 (石蜡切片需做抗原修复)
1mg/ml
Store at -20 °C
50ul 100ul 200ul
英文名称 | CEBP-alpha |
中文名称 | 转录调节因子C/EBP α 抗体 |
别 名 | Apoptotic cysteine protease; Apoptotic protease Mch 5; C/EBP alpha; CAP4; Caspase 8 precursor; CCAAT Enhancer Binding Protein alpha; CEBP; CEBP A; CEBP alpha; CEBPA; FADD homologous ICE/CED 3 like protease; FADD like ICE; FLICE; ICE like apoptotic protease 5; ICE8; MACH; MCH5; MORT1 associated CED 3 homolog; CEBP α, CEBP-α. |
规格价格 | 50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
说 明 书 | 50ul 100ul 200ul |
研究领域 | 肿瘤 心血管 染色质和核信号 转录调节因子 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, |
产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/test IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 39kDa |
细胞定位 | 细胞核 |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human CEBP-alpha:251-358/358 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
PubMed | PubMed |
产品介绍 | background: The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. [provided by RefSeq, Jul 2008]. Function: C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. Subunit: Interacts with PRDM16. Binds DNA as a dimer and can form stable heterodimers with C/EBP beta and gamma. Interacts with UBN1. Interacts with HBV protein X. Interacts with ZNF638; this interaction increases transcriptional activation. Subcellular Location: Nucleus. Similarity: Belongs to the bZIP family. C/EBP subfamily. Contains 1 bZIP domain. SWISS: P49715 Gene ID: 1050 Database links: Entrez Gene: 1050 Human Entrez Gene: 12606 Mouse Entrez Gene: 24252 Rat Omim: 116897 Human SwissProt: P49715 Human SwissProt: P53566 Mouse SwissProt: P05554 Rat Unigene: 76171 Human Unigene: 349667 Mouse Unigene: 153603 Rat Unigene: 204833 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品图片 | Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CEBP-alpha Polyclonal Antibody, Unconjugated(bs-1630R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CEBP-alpha Antibody(bs-1630R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange). |
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