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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
嵘崴达
- 库存:
需确认
- 英文名:
Recombinant Human IFN-gamma
- 规格:
100ug
产品概述
IFN-γ is an acid-labile interferon produced by CD4 and CD8 T lymphocytes as well as activated NK cells. IFN-γ receptors are present in most immune cells, which respond to IFN-γ signaling by increasing the surface expression of class I MHC proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. IFN-γ signaling in antigen-presenting cells, and antigen-recognizing B and T lymphocytes, regulates the antigen-specific phases of the immune response. Additionally, IFN-γ stimulates a number of lymphoid cell functions, including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils. Human IFN-γ is species-specific and is biologically active only in human and primate cells. Recombinant Human IFN-γ is a 16.8 kDa protein containing 144 amino acid residues.
Source:E.coli
Synonyms:Immune Interferon, type II interferon, T cell interferon, MAF
AA Sequence:MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ
Purity:≥ 98% by SDS-PAGE gel and HPLC analyses.
Biological Activity:Assay #1: Determined by its ability to induce STAT1/STAT2 activation in Human COLO 205 ISRE LUC reporter cells.
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文献和实验Preparation of Fluorescent DNA Probe from HUMAN mRNA or Total RNA using Direct Incorporation
I. Preparing fluoresenctly labeled cDNA (probe): To anneal primer, mix 2ug of mRNA or 50-100m g total RNA with 4ug of a regular or anchored oligo-dT primer in a total volume of 15.4 ul:
【分享】Human press METHODS IN MOLECULAR BIOLOGY
9. Protocols in Human Molecular Genetics. Edited by G. Methaw, 1991 10. Immunochemical Protocols. Edited by Manson, Margaret M., 1992 11. Practical Protein Chromatography Edited by Kenney, Andrew, 1992 12. Pulsed-Field Gel Electrophoresis Protocols
Preparation of Fluorescent DNA Probe from HUMAN mRNA or Total RNA using Direct Incorp
Preparation of Fluorescent DNA Probe from HUMAN mRNA or Total RNA using Direct Incorporation (Max Diehn/Ash Alizadeh protocol; 3/15/01) I. Preparing fluoresenctly labeled cDNA (probe): To anneal primer, mix 2ug of mRNA or 50
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