培养基DMEM/F-12，高糖，无酚红 Thermo 110

货号： 11039021

规格

Glutamine:	L-Glutamine
Phenol Red Indicator:	No Phenol Red
Form:	Liquid
HEPES Buffer:	HEPES
Sodium Pyruvate Additive:	Sodium Pyruvate
Serum Supplementation:	Standard Serum Supplementation
Volume:	500 ml
Glucose:	High Glucose
Product Size:	500 mL
Green Features:	Sustainable packaging
Shipping Condition:	Room Temperature

内容及储存
Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

培养基配方
11039 - DMEM/F-12, HEPES, no phenol red

描述

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. Life Technologies offers a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM/F-12 is modified as follows:

With	Without
• L-glutamine	• Phenol Red
• HEPES

The complete formulation is available.

Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.

cGMP manufacturing and quality system
For supply chain continuity, Life Technologies manufactures DMEM/F-12 at two separate facilities, located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.