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- 11995065
- 美国
- 2025年07月07日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 保质期：
已收到实际货物为准
- 供应商：
北京诺博莱德科技有限公司
- 保存条件：
已收到实际货物为准
货号： 11995065
规格
| Glutamine: | L-Glutamine |
|---|---|
| Phenol Red Indicator: | Phenol Red |
| Form: | Liquid |
| Glucose: | High Glucose |
| Serum Supplementation: | Standard Serum Supplementation |
| HEPES Buffer: | No HEPES |
| Sodium Pyruvate Additive: | Sodium Pyruvate |
| Volume: | 500 ml |
| Product Size: | 500 mL |
| Green Features: | Sustainable packaging |
| Shipping Condition: | Room Temperature |
描述
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM is modified as follows:
| With | Without |
| • High Glucose | • HEPES |
| • L-glutamine | |
| • Phenol Red | |
| • Sodium Pyruvate |
The complete formulation is available.
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.
Product Use
For human ex vivo tissue and cell culture processing applications. CAUTION: When used as a medical device, Federal law restricts this device to sale by or on the order of a physician. Customers using Gibco® DMEM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference our Type II Drug Master File (DMF).
cGMP Manufacturing and Quality System
Gibco® DMEM is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers an identical Gibco® DMEM product made in our Scotland facility (41966-029). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
在中国境内, 本产品仅限于非临床科研用途, 产品信息以中文为准。 (In China territory, the product is for Research Use only in non-clinical unit, product information in Chinese shall prevail.)
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文献和实验的(小牛血清)RPMI培养基 4. 肝素钠钠盐(HEPES，最低99.5％，Sigma -Aldrich公司) 5. Ⅱ型胶原酶 6. 皂素(Sigma公司，＃S-7900) 7. BSA(牛血清白蛋白) 8. Azida(2mM) 实验设备 1. 剪刀 2. 钳子 3. 培养皿 4. 1.5，2.0，15和50毫升管 5. 热孵化器（振荡
基质（CC131），稀释比例为1:80。 根据所需的体积按相应比例上调或下调。 以下是用1.5 mL 1:80稀释的ECM凝胶包被6孔板的示例。 首先进行1:20稀释，操作步骤是，在15 mL锥形管中，将0.5 mL ECM凝胶加入9.5 mL冷的DMEM / F12或DMEM培养基中。 总体积 = 10 mL. 需要进一步稀释4倍才能稀释成最终的1:80。 将2.5 mL 1:20稀释的ECM凝胶加入7.5 mL冷的DMEM / F12培养基中。 总体积 = 10 mL 注意：建议的稀释度为1:80
mM的HEPES。 你查一下HEPES的分子量，就知道怎么配了。低血清培养基，就是培养基里面加入的血清比较少，eg：我要配0.1%血清的培养基，就是100ml的培养基加入0.1ml的血清。 24、流式细胞的问题： 问：细胞用一抗包被后，然后再和二抗结合，不知道这样能否用流式细胞检测？由于我要检测的蛋白在国内外没有可用于流式细胞检测的现货，有个公司虽然可制备，但需要6个月，并且价格一个蛋白要5万元以上，时间和经费均不足，只好想想其它办法，不知道这样可否？ 答：当然可以先用自己的一抗，再用荧光标记的二抗
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