- ¥1380 - 2200
- 康朗生物
- kl-6701R
- 中国/美国/德国
- 2025年12月16日
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500
- Rabbit
- Human, Mouse, Rat, Cow,
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海康朗生物科技有限公司
- 库存：
大量
- 目录编号：
kl-6701R
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
12个月
- 抗体英文名：
Calpastatin antibody
- 抗体名：
钙蛋白酶抑制蛋白抗体
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat, Cow,
- 免疫原：
KLH conjugated synthetic peptide derived from human Calpastatin:431-530/708
- 亚型：
IgG
- 形态：
冻干粉或液体
- 应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500
- 浓度：
1mg/ml
- 保存条件：
-20 °C
- 规格：
100ul 200ul
| 中文名称 | 钙蛋白酶抑制蛋白抗体 |
| 别 名 | BS 17; Calpain inhibitor; Calpastatin; CAST; Heart type calpastatin; ICAL_HUMAN; MGC9402; Sperm BS 17 component; Sperm BS-17 component. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 细胞生物 神经生物学 细胞类型标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Cow, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 70kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Calpastatin:431-530/708 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Calpains are nonlysosomal, calcium-activated intracellular cysteine proteases that mediate specific Ca2+-dependent processes including cell fusion, mitosis and meiosis. Calpains are heterodimers of a small regulatory subunit and one of three large catalytic subunits, designated Calpain 1, Calpain 2 and Calpain p94. Calpain 1 is an intracellular calcium-dependent protease that cleaves cytoskeletal and submembranous proteins. Calpain-1 co-localizes with human leukocyte antigen-DR (HLA-DR) on activated microglia in the aging brain. Cal-pain influences the process of spermatogenesis and the events preceding fertilization, such as the acrosome reaction. Calpastatin regulates Calpain by inhibiting both the proteolytic activity of Calpain and its binding to membranes. Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing. Function: Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue. Post-translational modifications: The N-terminus is blocked. Similarity: Belongs to the protease inhibitor I27 (calpastatin) family. SWISS: P20810 Gene ID: 831 Database links: Entrez Gene: 831 Human Entrez Gene: 12380 Mouse Entrez Gene: 25403 Rat Omim: 114090 Human SwissProt: P20810 Human SwissProt: P51125 Mouse SwissProt: P27321 Rat Unigene: 436186 Human Unigene: 29163 Mouse Unigene: 441995 Mouse Unigene: 17481 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti-Calpastatin (bs-6701R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 70 kD Observed band size: 65 kD  Sample: Raji Cell (Human) Lysate at 40 ug Primary: Anti-Calpastatin (bs-6701R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 70 kD Observed band size: 65 kD |
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文献和实验Preparation of Calpastatin Samples for Western Blotting
Determination of calpastatin levels in cells or tissues by Western blotting using antibodies, or by assay of calpain inhibitory activities in vitro, may be required to assess the role and the level of calpastatin, with respect to the levels
Purification of Recombinant Calpastatin Expressed in Escherichia coli
Calpastatin is an endogenous inhibitor protein specific for calpain (1 ,2 ). Other calpain-inhibiting reagents such as EDTA, E-64, leupeptin, and calpain inhibitor I (N -acetyl-L-leucyl-L-leucyl-L-norleucinal), are frequently used
Purification of - and m-Calpain and Calpastatin from Animal Tissues
This chapter describes procedures for purification of μ-calpain, m-calpain, and calpastatin simultaneously from a single tissue sample. The procedures and reagents described apply equally well to purification
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