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TUBA1C antibody

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  • ¥1380 - 2200
  • 康朗生物
  • kl-5118R
  • 中国/美国/德国
  • 2025年11月28日
  • WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500
  • Rabbit
  • Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit,
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    • 详细信息
    • 技术资料
    • 供应商

      上海康朗生物科技有限公司

    • 库存

      大量

    • 目录编号

      kl-5118R

    • 克隆性

      多克隆

    • 抗原来源

      Rabbit

    • 保质期

      12个月

    • 抗体英文名

      TUBA1C antibody

    • 抗体名

      微管相关蛋白α6抗体

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit,

    • 免疫原

      KLH conjugated synthetic peptide derived from human Tubulin alpha 6:51-150/449

    • 亚型

      IgG

    • 形态

      冻干粉或液体

    • 应用范围

      WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500

    • 浓度

      1mg/ml

    • 保存条件

      -20 °C

    • 规格

      100ul 200ul

    TUBA1C antibody
    中文名称 微管相关蛋白α6抗体
    别    名 Alpha tubulin 6; Alpha tubulin isotype M alpha 6; bcm948; M[a]6; TUBA1C; Tubulin alpha 1C chain; Tubulin alpha-1C chain; tubulin alpha 6; Tubulin alpha 6 chain; tubulin, alpha 1c; TBA1C_HUMAN; Alpha-tubulin 6; Tubulin alpha-6 chain.   
    规格价格 100ul/1380元 购买    200ul/2200元 购买    大包装/询价
    说 明 书 100ul  200ul
    研究领域 肿瘤  细胞生物  免疫学  转录调节因子  
    抗体来源 Rabbit
    克隆类型 Polyclonal
    交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, 
    产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复) 
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    分 子 量 49kDa
    细胞定位 细胞浆 
    性    状 Lyophilized or Liquid
    浓    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from human Tubulin alpha 6:51-150/449 
    亚    型 IgG
    纯化方法 affinity purified by Protein A
    储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    PubMed PubMed
    产品介绍 background:
    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

    Function:
    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

    Subunit:
    Dimer of alpha and beta chains.

    Subcellular Location:
    Cytoplasm, cytoskeleton.

    Post-translational modifications:
    Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively. 
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules (Probable). 
    Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.

    Similarity:
    Belongs to the tubulin family.

    SWISS:
    Q9BQE3

    Gene ID:
    84790

    Database links:

    Entrez Gene: 84790 Human

    Entrez Gene: 504244 Cow

    Entrez Gene: 22146 Mouse

    Entrez Gene: 300218 Rat

    SwissProt: Q3ZCJ7 Cow

    SwissProt: Q9BQE3 Human

    SwissProt: P68373 Mouse

    SwissProt: Q6AYZ1 Rat

    Unigene: 725987 Human

    Unigene: 88212 Mouse

    Unigene: 154526 Rat



    Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
     
    产品图片 产品细节图片1
    Sample: 
    Cerebrum (Mouse) Lysate at 40 ug
    Primary: Anti-TUBA1C (bs-5118R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 49 kD
    Observed band size: 49 kD

    产品细节图片2
    Sample:
    A549 Cell (Human) Lysate at 30 ug
    Primary: Anti- TUBA1C (bs-5118R)at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 49kD
    Observed band size: 49kD

    产品细节图片3
    Sample: 
    K562(Human) Cell Lysate at 40 ug
    Hela(Human) Cell Lysate at 40 ug
    Primary: Anti-TUBA1C (bs-5118R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 49 kD
    Observed band size: 49 kD

    产品细节图片4
    Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TUBA1C) Polyclonal Antibody, Unconjugated (bs-5118R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片5
    Blank control(blue): RSC96 (fixed with 2% paraformaldehyde (10 min),then permeabilized with 90% ice-cold methanol for 30 min on ice). 
    Primary Antibody:Rabbit Anti- TUBA1C antibody(bs-5118R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; 
    Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); 
    Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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