GLI1脑胶质瘤相关蛋白抗体（锌指蛋白5）

价格¥780 - 2200

品牌LMAI Bio
货号LM-1206R
产地进口/国产
更新日期2025年07月15日

应用范围WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/Test IF=1:100-500 （石蜡切片需做抗原修复）
宿主Rabbit
适应物种Human, Mouse, Rat, Dog, Cow, Horse,

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供应商：
上海联迈生物工程有限公司
库存：
大量
目录编号：
LM-1206R
克隆性：
多克隆
抗原来源：
Rabbit
保质期：
1年
抗体英文名：
GLI1
抗体名：
脑胶质瘤相关蛋白抗体（锌指蛋白5）
宿主：
Rabbit
适应物种：
Human, Mouse, Rat, Dog, Cow, Horse,
免疫原：
KLH conjugated synthetic peptide derived from human GLI1/Zfp5:601-700/1106
亚型：
IgG
形态：
Lyophilized or Liquid
应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/Test IF=1:100-500 （石蜡切片需做抗原修复）
浓度：
1mg/ml
保存条件：
Store at -20 °C
规格：
50ul 100ul 200ul

GLI1脑胶质瘤相关蛋白抗体（锌指蛋白5）

英文名称	GLI1
中文名称	脑胶质瘤相关蛋白抗体（锌指蛋白5）
别名	Gli 1; Gli1; Gli-1; GLI; GLI Kruppel family member 1; Glioma associated oncogene; Glioma associated oncogene homolog 1 (zinc finger protein); Oncogene GLI; Zfp 5; Zfp5; Zinc finger protein GLI 1; Zinc finger protein GLI1; GLI1_HUMAN.
	Specific References (6) \| bs-1206R has been referenced in 6 publications. [IF=2.15] Fan, Hai-Xia, et al. "Sonic hedgehog signaling may promote invasion and metastasis of oral squamous cell carcinoma by activating MMP-9 and E-cadherin expression." Medical Oncology 31.7 (2014): 1-8. IHC-P ; Human. PubMed:24915900 [IF=2.30] Ma, Zhenkun, et al. "Silibinin induces apoptosis through inhibition of the mTOR-GLI1-BCL2 pathway in renal cell carcinoma." Oncology Reports. other ; PubMed:26323996 [IF=2.88] Long, B., et al. "Targeting GLI1 Suppresses Cell Growth and Enhances Chemosensitivity in CD34+ Enriched Acute Myeloid Leukemia Progenitor Cells." Cellular Physiology and Biochemistry 38.4 (2016): 1288-1302. Human. PubMed:27008269 [IF=4.46] Du, Wen‐Zhong, et al. "Curcumin Suppresses Malignant Glioma Cells Growth and Induces Apoptosis by Inhibition of SHH/GLI1 Signaling Pathway in Vitro and Vivo." CNS Neuroscience & Therapeutics (2013). Human. PubMed:24165291 [IF=3.73] Zhang, Qiang, et al. "Serotonin Receptor 2C and Insulin Secretion." PloS one 8.1 (2013): e54250. WB ; Mouse. PubMed:23349838 [IF=2.40] Yue, Yongjian, et al. "Aberrant activation of Hedgehog pathway in Nasopharyngeal carcinoma." Clinical and Experimental Medicine (2012): 1-8. IHC-P ; Human. PubMed:23001130
规格价格	50ul/780元购买 100ul/1380元购买 200ul/2200元购买大包装/询价
说明书	50ul 100ul 200ul
研究领域	肿瘤细胞生物神经生物学信号转导表观遗传学
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human, Mouse, Rat, Dog, Cow, Horse,
产品应用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/Test IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	118kDa
细胞定位	细胞核细胞浆
性状	Lyophilized or Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human GLI1/Zfp5:601-700/1106
亚型	IgG
纯化方法	affinity purified by Protein A
储存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed	PubMed
产品介绍	background: This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009] Function: Acts as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation. Subcellular Location: Cytoplasm. Nucleus. Tethered in the cytoplasm by binding to SUFU. Activation and translocation to the nucleus is promoted by interaction with STK36. Phosphorylation by ULK3 may promote nuclear localization. Translocation to the nucleus is promoted by interaction with ZIC1. Tissue Specificity: Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract. SWISS: P08151 Gene ID: 2735 Database links: Entrez Gene: 2735 Human Entrez Gene: 14632 Mouse Entrez Gene: 140589 Rat Entrez Gene: 517588 Cow Omim: 165220 Human SwissProt: P08151 Human SwissProt: P47806 Mouse Unigene: 632702 Human Unigene: 391450 Mouse Unigene: 219157 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. GLI1是一种具有很强活性的转录激活因子，GLI1诱导G1/S细胞周期调节蛋白的表达从而促进细胞的增殖; 直接诱导抗凋亡因子Bcl-2的表达以抑制凋亡; 直接激活促进上皮组织向间质转化因子的转录从而加重了肿瘤的侵袭性。目前主要用于肿瘤及神经方面的研究。
产品图片	Sample: Uterus(Mouse)Lysate at 40 ug Ovary (Mouse)Lysate at 40 ug Primary: Anti-GLI1(bs-1206R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 118kD Observed band size: 130kD Sample:HepG2 (Human)Cell Lysate at 40 ug Primary: Anti-GLI1(bs-1206R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 118kD Observed band size: 130kD Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLI1) Polyclonal Antibody, Unconjugated (bs-1206R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLI1) Polyclonal Antibody, Unconjugated (bs-1206R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLI1) Polyclonal Antibody, Unconjugated (bs-1206R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLI1) Polyclonal Antibody, Unconjugated (bs-1206R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation: Anti-GLI1 Antibody, conjugated (bs-1206R-AF555) at 1:100 overnight at 4°C; DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation: Anti-GLI1 Antibody, conjugated (bs-1206R-AF555) at 1:100 overnight at 4°C; DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei. Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA . Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).