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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海联迈生物工程有限公司
- 库存:
大量
- 目录编号:
LM-12035R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
1年
- 抗体英文名:
Paralemmin 1
- 抗体名:
细胞膜调控蛋白Paralemmin 1抗体
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat, Dog, Pig, Cow, Sheep,
- 免疫原:
KLH conjugated synthetic peptide derived from human Paralemmin 1:130-230/387
- 亚型:
IgG
- 形态:
Lyophilized or Liquid
- 应用范围:
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
- 浓度:
1mg/ml
- 保存条件:
Store at -20 °C
- 规格:
100ul 200ul
| 英文名称 | Paralemmin 1 |
| 中文名称 | 细胞膜调控蛋白Paralemmin 1抗体 |
| 别 名 | KIAA0270; PALM; PALM_HUMAN; Paralemmin; Paralemmin1;Paralemmin-1. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 细胞生物 神经生物学 信号转导 G蛋白偶联受体 G蛋白信号 细胞膜蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Sheep, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 42kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Paralemmin 1:130-230/387 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Paralemmin, also called Paralemmin-1 or PALM, is a widely expressed peripheral membrane protein that is involved in cell structure and shape. A hydrophobic protein, Paralemmin is anchored to the cytoplasmic side of the cell membrane via di-palmitoylation and prenylation of its C-terminal cysteine cluster. Functioning at the synapse to regulate neuronal plasticity and plasma membrane dynamics, Paralemmin can bind to the dopamine receptor D3, thereby reducing D3 expression and subsequent adenylate cyclase activity. Overexpression of Paralemmin induces fibroblasts to extend long filopodia and to assume extreme cell shapes, suggesting involvement in the formation and stabilization of the plasma membrane. Two isoforms of Paralemmin exists due to alternative splicing events. Function: Involved in plasma membrane dynamics and cell process formation. Isoform 1 and isoform 2 are necessary for axonal and dendritic filopodia induction, for dendritic spine maturation and synapse formation in a palmitoylation-dependent manner. Subunit: Interacts with dopamine receptor DRD3. Subcellular Location: Cell membrane; Lipid-anchor; Cytoplasmic side. Cell projection, filopodium membrane; Lipid-anchor. Cell projection, axon (By similarity). Cell projection, dendrite (By similarity). Cell projection, dendritic spine (By similarity). Basolateral cell membrane; Lipid-anchor (By similarity). Apicolateral cell membrane; Lipid-anchor (By similarity). Note=Translocation to the plasma membrane is enhanced upon stimulation of neuronal activity. Tissue Specificity: Widely expressed with highest expression in brain and testis and intermediate expression in heart and adrenal gland. Similarity: Belongs to the paralemmin family. SWISS: O75781 Gene ID: 5064 Database links: Entrez Gene: 5064 Human Entrez Gene: 18483 Mouse Entrez Gene: 170673 Rat Omim: 608134 Human SwissProt: O75781 Human SwissProt: Q9Z0P4 Mouse SwissProt: Q920Q0 Rat Unigene: 631841 Human
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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文献和实验表达基因的区域,这部分打开的染色质,就叫开放染色质(open chromatin)。打开染色质,允许部分调控蛋白(比如转录因子和辅因子)与之相结合的特性,即染色质的可及性(chromatin accessibility)。 对染色质可及性探究的方法有很多,比如DNase-seq,MNase-seq,ChIP-seq,FAIRE-seq等。DNase-seq和MNase-seq方法利用限制性酶对染色质DNA进行片段化处理,该方法不能获得完整开放的染色质信息。相较于粗暴的核酸酶,ScATAC
氏体到达细胞膜,在此过程中被糖基化。 五、装配与释放 新合成的病毒核酸和病毒结构蛋白在感染细胞内组合成病毒颗粒的过程称为装配(Assembly),而从细胞内转移到细胞外的过程为释放(Release)。大多数DNA病毒,在核内复制DNA,在胞浆内合成蛋白质,转入核内装配成熟。而痘苗病毒其全部成份及装配均在胞浆内完成。RNA病毒多在胞浆内复制核酸及合成蛋白。感染后6个小时,一个细胞可产生多达10,000个病毒颗粒。 病毒装配成熟后释放的方式有:(1)宿主细胞裂解,病毒
激酶完成磷酸化,这是活化或灭活某些蛋白的一种方式。病毒糖蛋白是在胞浆中与膜相连的核糖体上合成,经粗面内质网、平滑内质网、高尔基氏体到达细胞膜,在此过程中被糖基化。 五、装配与释放 新合成的病毒核酸和病毒结构蛋白在感染细胞内组合成病毒颗粒的过程称为装配(Assembly),而从细胞内转移到细胞外的过程为释放(Release)。大多数DNA病毒,在核内复制DNA,在胞浆内合成蛋白质,转入核内装配成熟。而痘苗病毒其全部成份及装配均在胞浆内完成。RNA病毒多在胞浆内复制核酸及合成蛋白。感染
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