- ¥780 - 2200
- LMAI Bio
- LM-1056R
- 进口/国产
- 2025年07月15日
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=3ug/Test IF=1:200-800 （石蜡切片需做抗原修复）
- Rabbit
- Human, Mouse, Rat,
10 年钻石会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 目录编号：
LM-1056R
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
5HT2A Receptor
- 抗体名：
5-羟色胺受体2A抗体
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat,
- 免疫原：
KLH conjugated synthetic peptide derived from human 5HT2A Receptor:65-160/471 <Cytoplasmic>
- 亚型：
IgG
- 形态：
Lyophilized or Liquid
- 应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=3ug/Test IF=1:200-800 （石蜡切片需做抗原修复）
- 浓度：
1mg/ml
- 保存条件：
Store at -20 °C
- 规格：
50ul 100ul 200ul
5HT2A Receptor5-羟色胺受体2A抗体
| 英文名称 | 5HT2A Receptor |
| 中文名称 | 5-羟色胺受体2A抗体 |
| 别 名 | 5-hydroxytryptamine receptor 2A; 5-hydroxytryptamine receptor; Serotonin receptor 2A; 5-HT-2A; 5-HT-2; HTR2A; Htr2a; 5 HT 2; 5HTR2A; 5 HT 2A; 5 HT2 receptor; 5 HT2A; 5 hydroxytryptamine receptor 2A; 5-HT-2; 5-HT-2A; 5-hydroxytryptamine 2A receptor; 5-hydroxytryptamine receptor 2A; 5HT2A_HUMAN; HTR 2; HTR 2A; HTR2; HTR2A. |
 |
Specific References (1) | bs-1056R has been referenced in 1 publications. [IF=2.59] Sun, Tao, et al. "Antihypertensive effect of formononetin through regulating the expressions of eNOS, 5-HT< sub> 2A/1B receptors and ??< sub> 1-adrenoceptors in spontaneously hypertensive rat arteries."European journal of pharmacology (2013).. Rat. PubMed:23123056 |
| 规格价格 | 50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 50ul 100ul 200ul |
| 研究领域 | 神经生物学 通道蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=3ug/Test IF=1:200-800 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 52kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human 5HT2A Receptor:65-160/471 <Cytoplasmic> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: 5HT2A receptor is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. 5HT2A receptor belongs to the G-protein coupled receptor 1 family and mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. This receptor is involved in tracheal smooth muscle contraction, bronchoconstriction, and control of aldosterone production. 5HT2A receptor is an integral membrane protein which localizes to the post-synaptic thickening of axo-dendritic synapses. 5HT2A receptor protein contains a PDZ domain-binding motif which is involved in the interaction with INADL, CASK, APBA1, DLG1 and DLG4. Function: This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. This receptor is involved in tracheal smooth muscle contraction, bronchoconstriction, and control of aldosterone production. Subunit: Interacts with MPDZ and INADL. May interact with MPP3, PRDX6, DLG4, DLG1, CASK, APBA1 and MAGI2. Subcellular Location: Cell membrane; Multi-pass membrane protein. Note=Localizes to the postsynaptic thickening of axo-dendritic synapses. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P28223 Gene ID: 3356 Database links: Entrez Gene: 3356 Human Entrez Gene: 15558 Mouse Entrez Gene: 29595 Rat Omim: 182135 Human SwissProt: P28223 Human SwissProt: P35363 Mouse SwissProt: P14842 Rat Unigene: 654586 Human Unigene: 72630 Human Unigene: 214351 Mouse Unigene: 10294 Rat Unigene: 146756 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Sample: U251(Human) Cell Lysate at 30 ug BV2(Mouse) Cell Lysate at 30 ug Primary: Anti-5HT2A Receptor (bs-1056R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD  Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (5HT2A Receptor) Polyclonal Antibody, Unconjugated (bs-1056R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.  Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (5HT2A Receptor) Polyclonal Antibody, Unconjugated (bs-1056R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (5HT2A Receptor) Polyclonal Antibody, Unconjugated (bs-1056R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.  Tissue/cell: rat lung tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-5HT2A Receptor Polyclonal Antibody, Unconjugated(bs-1056R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.  Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (5HT2A Receptor) Polyclonal Antibody, Unconjugated (bs-1056R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G- cy3) for 90 minutes, and DAPI for nuclei staining.  Blank control: U-2OS. Primary Antibody (green line): Rabbit Anti-5HT2A Receptor antibody (bs-1056R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 3μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
受体在细胞生物学中是一个很泛的概念,意指任何能够同激素、神经递质、药物或细胞内的信号分子结合并能引起细胞功能变化的生物大分子。 在细胞通讯中,由信号传导细胞送出的信号分子必须被靶细胞接收才能触发靶细胞的应答,接收信息的分子称为受体,此时的信号分子被称为配体(ligand)。在细胞通讯中受体通常是指位于细胞膜表面或细胞内与信号分子结合的蛋白质。
的肽激素作用于同一脂肪细胞时，存在不同的受体，每种激素分别与之结合。因位于膜上，故与处在同一膜上的腺苷酸环化酶有关。甾类化合物激素的受体称为胞液受体（cytos-ol-receptor），存在于细胞质中，至少其活性部分是蛋白质。关于病毒受体的概念，为了说明病毒的宿主范围，病毒与细胞表面间有抗原抗体反应的互补结构，1929年伯内特（F.M.Burnet）开始研究细胞病毒，发现流感病毒有凝聚红血球的反应，并以此为转机，扩大到其它病毒系统。病毒受体是与病毒粒子特异结合引起病毒侵入和脱外壳反应的结构体
乙 -受体（β -受体） β -receptor 儿茶酚胺的受体之一。一般呈抑制反应，即一旦接受儿茶酚胺的作用，便产生血管、子宫、支气管等器管的舒张和心肌兴奋。由异丙基肾上腺素（ isopr-oterenol）的作用也可产生这种反应。而 3， 4-二氯异丙基肾上腺素（ DCI）、心得安等β -受体阻断剂可阻断其作用。
技术资料暂无技术资料 索取技术资料
5HT2A Receptor5-羟色胺受体2A抗体
¥780 - 2200
