CK19 antibody

CK19 antibody

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  • ¥1380 - 2200
  • 康朗生物
  • kl-15590R
  • 中国/美国/德国
  • 2025年07月10日
  • WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500
  • Rabbit
  • Human
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    • 详细信息
    • 技术资料
    • 供应商

      上海康朗生物科技有限公司

    • 库存

      大量

    • 目录编号

      kl-15590R

    • 克隆性

      多克隆

    • 抗原来源

      Rabbit

    • 保质期

      12个月

    • 抗体英文名

      CK19 antibody

    • 抗体名

      细胞角蛋白19抗体

    • 宿主

      Rabbit

    • 适应物种

      Human

    • 免疫原

      KLH conjugated synthetic peptide derived from human CK19 :301-400/400

    • 亚型

      IgG

    • 形态

      冻干粉或液体

    • 应用范围

      WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500

    • 浓度

      1mg/ml

    • 保存条件

      -20 °C

    • 规格

      100ul 200ul

    CK19 antibody
    中文名称 细胞角蛋白19抗体
    别    名 K1C19_HUMAN; Keratin, type I cytoskeletal 19; Cytokeratin 19; Cytokeratin-19; CK-19; Keratin-19; CYFRA21-1; K19.  
    规格价格 100ul/1380元 购买    200ul/2200元 购买    大包装/询价
    说 明 书 100ul  200ul
    研究领域 肿瘤  细胞生物  免疫学  肿瘤细胞生物标志物  
    抗体来源 Rabbit
    克隆类型 Polyclonal
    交叉反应 Human, 
    产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复) 
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    分 子 量 30kDa
    性    状 Lyophilized or Liquid
    浓    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from human CK19 :301-400/400 
    亚    型 IgG
    纯化方法 affinity purified by Protein A
    储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    PubMed PubMed
    产品介绍 background:
    The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008].

    Function:
    Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

    Subunit:
    Heterotetramer of two type I and two type II keratins. Interacts with PNN and the actin-binding domain of DMD. Interacts with HCV core protein.

    Tissue Specificity:
    Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.

    Similarity:
    Belongs to the intermediate filament family.

    SWISS:
    P08727

    Gene ID:
    3880

    Database links:

    Entrez Gene: 3880 Human

    Entrez Gene: 16669 Mouse

    Omim: 148020 Human

    SwissProt: P08727 Human

    SwissProt: P19001 Mouse

    Unigene: 654568 Human

    Unigene: 439699 Mouse



    Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
     
    产品图片 CK19 antibody
    Paraformaldehyde-fixed, paraffin embedded (Human stomach cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK19) Polyclonal Antibody, Unconjugated (bs-15590R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    CK19 antibody
    Blank control (blue line): Hela (fixed with 70% methanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃). 
    Primary Antibody (green line): Rabbit Anti-CK19 antibody (bs-15590R),Dilution: 0.2μg /10^6 cells; 
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test. 

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