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- 11865471103
- 2025年08月25日
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- 技术资料
- 供应商：
嵘崴达
- 保质期：
见产品外包装
- 保存条件：
见说明书
- 英文名：
Creatininase, Recombinant Lyo
- 库存：
需确认
Creatininase 肌酐酶-11865471103
from Pseudomonas species, expressed in E.coli, lyo-
philizate
Hydrolase for creatinine determination that catalyzes the conversion of creati-
nine to creatine.
Application
Use Creatininase as a diagnostic reagent for the determination of creatine
in combination with Creatinase, Catalog No. 11 799 142 103 and Sarcosine
Oxidase, Catalog No. 11 378 856 103.
Benefits
Rely on the proven diagnostic quality of this product.
EC 3.5.2.10
Properties
Nomenclature: Creatinine amidohydrolase
Molecular weight (gel filtration): 175 kD
Structure (SDS PAGE): 8 equal subunits (23 kD + zinc)
Isoelectric point (IEF): 4.7
Michaelis constants (Glycylglycine buffer, pH 8.0, +25°C):
Creatinine: 3 x 10 -2 mol/l
Creatine: 6 x 10 -2 mol/l
Inhibitors: Hg 2+ , Fe 3+ , Cu 2+ (1 mmol/l), N-bromosuccinimide, o-phenanthron-
line, 4-chloromercuribenzoate. The enzyme is sensitive against photooxida-
tion. A stable, inactive apoenzyme free of zinc can be obtained after EDTA
incubation which can be reactivated completely with Zn 2+ , Mn 2+ , Mg 2+ , Co 2+ ,
Fe 2+ or Ni 2+ (1 mmol/l).
Activators: Mn 2+ , Mg 2+ . The enzyme requires metal ions. Phenylmethylsulfo-
nylfluoride and iodoacetamide do not react.
pH optimum: 7.8 (see figure)
Temperature dependence: See figure
pH stability: 7.5-9.0 (see figure
Thermal stability: Up to +65°C (see figure)
Specificity: Creatininase is specific for creatinine. It also reacts with glycocya-
midine and glycocyamine. It does not react with hydantoin and its derivatives.
Specification
Appearance: White to slightly yellowish lyophilizate
Solubility: Clear, colorless to slightly yellowish solution in water (c=10 mg/ml)
pH value (c=10 mg/ml in water): 7.0-8.0
Activity (+25°C, creatinine): ≥55 U/mg lyophilizate
Specific activity: ≥250 U/mg protein
Protein (Biuret): 0.10-0.35 mg/mg lyophilizate
Contaminants (expressed as percentage of Creatininase activity):
ATPase: ≤0.01
Catalase: ≤2.0
Contaminating oxidases (FOX:) ≤0.001
Creatinine deaminase: ≤0.0015
Kinase test: ≤0.01
Proteases (casein/resorufin, 2 hours stress duration): ≤0.005
Stability: At +2 to +8°C within specification range for 12 months. Protect from
light.
from Pseudomonas species, expressed in E.coli, lyo-
philizate
Hydrolase for creatinine determination that catalyzes the conversion of creati-
nine to creatine.
Application
Use Creatininase as a diagnostic reagent for the determination of creatine
in combination with Creatinase, Catalog No. 11 799 142 103 and Sarcosine
Oxidase, Catalog No. 11 378 856 103.
Benefits
Rely on the proven diagnostic quality of this product.
EC 3.5.2.10
Properties
Nomenclature: Creatinine amidohydrolase
Molecular weight (gel filtration): 175 kD
Structure (SDS PAGE): 8 equal subunits (23 kD + zinc)
Isoelectric point (IEF): 4.7
Michaelis constants (Glycylglycine buffer, pH 8.0, +25°C):
Creatinine: 3 x 10 -2 mol/l
Creatine: 6 x 10 -2 mol/l
Inhibitors: Hg 2+ , Fe 3+ , Cu 2+ (1 mmol/l), N-bromosuccinimide, o-phenanthron-
line, 4-chloromercuribenzoate. The enzyme is sensitive against photooxida-
tion. A stable, inactive apoenzyme free of zinc can be obtained after EDTA
incubation which can be reactivated completely with Zn 2+ , Mn 2+ , Mg 2+ , Co 2+ ,
Fe 2+ or Ni 2+ (1 mmol/l).
Activators: Mn 2+ , Mg 2+ . The enzyme requires metal ions. Phenylmethylsulfo-
nylfluoride and iodoacetamide do not react.
pH optimum: 7.8 (see figure)
Temperature dependence: See figure
pH stability: 7.5-9.0 (see figure
Thermal stability: Up to +65°C (see figure)
Specificity: Creatininase is specific for creatinine. It also reacts with glycocya-
midine and glycocyamine. It does not react with hydantoin and its derivatives.
Specification
Appearance: White to slightly yellowish lyophilizate
Solubility: Clear, colorless to slightly yellowish solution in water (c=10 mg/ml)
pH value (c=10 mg/ml in water): 7.0-8.0
Activity (+25°C, creatinine): ≥55 U/mg lyophilizate
Specific activity: ≥250 U/mg protein
Protein (Biuret): 0.10-0.35 mg/mg lyophilizate
Contaminants (expressed as percentage of Creatininase activity):
ATPase: ≤0.01
Catalase: ≤2.0
Contaminating oxidases (FOX:) ≤0.001
Creatinine deaminase: ≤0.0015
Kinase test: ≤0.01
Proteases (casein/resorufin, 2 hours stress duration): ≤0.005
Stability: At +2 to +8°C within specification range for 12 months. Protect from
light.
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肌酐酶-11865471103
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