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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
嵘崴达
- 英文名：
DNase I rec RGI
- 保存条件：
见说明书
- 库存：
需确认
- 保质期：
见产品外包装
- 规格：
10 KU
from bovine pancreas, expressed in Pichia pastoris, lyo-
philizate
Recombinant produced DNase I in PCR grade, lyophilized quality, free of
animal-derived materials, is an essential tool for all applications requiring
DNase-free RNA templates.
Application
DNase I, recombinant, Grade I, is suitable for:
Isolation of DNA-free RNA produced by ■ in vitro transcription
Producing DNA-free preparations of protein and RNA: ■
To ensure that RT-PCR templates are free of genomic DNA ■
To remove DNA templates after ■ in vitro transcription of RNA
Nick-translation labeling of DNA with added DNA polymerase I ■
Determining the "footprint" of a DNA-binding protein ■
Microarray analysis ■
Benefits
Be compliant with regulatory requirements. ■ Assure that your ap-
plications are free of animal-derived materials.
Achieve reliable results. ■ Experience excellent quality and greater lot-
to-lot consistency due to advanced production processes in conjunction
with rigorous analytical testing.
Product Description
DNase I, recombinant, Grade I, originally isolated from bovine pancreas, is
a recombinant enzyme expressed in Pichia pastoris. It is a glycoprotein of a
molecular weight of approximately 39 kD. DNase I, recombinant, Grade I, is
a DNA-specific endonuclease that hydrolyzes phosphodiester linkages of
double- and single-stranded DNA to a mixture of mono- and oligonucleotides.
DNase I, recombinant, Grade I, is manufactured using state-of-the-art pro-
cesses yielding animal-free material.
EC 3.1.21.1
Properties
Nomenclature: DNase I
pH optimum: 7.0-8.0
Activators: DNase I requires bivalent cations for maximal activity.
Inhibitors: EDTA, EGTA, SDS
Specificity: Double-strand specific endonuclease that degrades DNA
Specification
Appearance: White to slightly yellowish lyophilizate
Activity (calf thymus DNA): ≥10 kU/vial lyophilizate
Activity (calf thymus DNA, modified buffer system): No limit
Unit definition: One unit according to Kunitz produces an increase in absor-
bance of 0.001/minute under assay conditions in 1 ml at 260 nm.
Proteases (resorufin-marked casein): Not detectable in up to 50 U after 17
hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 2 U after 4 hours incuba-
tion at +37°C.
Stability: At +2 to +8°C within specification range for 12 months.
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文献和实验为对 DNA有特异作用的、可水解核苷酸间的磷酯键的具有解聚化作用的核酸酶（一种磷酸酯酶），缩写 DNase。可从各种生物和组织中分离出来，但这些酶在细胞中的作用尚不清楚。根据 DNase的作用机理，可分为二种，即可将分子链内部的磷酸二酯键水解的核酸内切酶（脱氧核糖酶Ⅰ等），以及对分子链末端分阶段作用生成单核苷酸的外切核酸酶。 DN- ase的测定方法有以下几种：（ 1）对伴有 DNA分解时的粘性下降的测定；（ 2）对由分解生成的酸溶性生成物量的测定（全磷量在 260毫微米时的吸收
最有代表性的核酸内切酶。最早是由 M． Kunitz从胰脏中分离出结晶的，也称为胰脱氧核糖核酸酶或 DNaseⅠ， EC3． 1． 21． 1。分子量约 3.1万，等电点 pH4.7，最适 PH 7附近，需要 Mg2 和 Mn2 。分离单链和双链 DNA，产生具有 5′ -磷酸末端的分解物。在一般条件下其分解产物含有 1到 8— 12个寡聚核苷酸，平均大小等于四个核苷酸。反应初期似乎是先分解 dpPupPy键，但因二价离子的存在对切断的方式有显著影响。因此酶容易得到结晶
微球菌脱氧核糖核酸酶 deoxyribonuclease micrococcal
在金黄色化脓微球菌（ Micrococcus pyoge－ nes var． aureus）等于金黄色葡萄球菌（ Staphylococ－ cus aureus）的培养液中发现的核酸内切酶，致病性越强的菌株产生的越多。最适 pH在 8.6附近，反应必须有 Ca2 （ 10mM），能水解单链和双链 DNA。此酶对热极稳定， 95℃加热 15分钟几乎不失活。反应开始可选择性地切断 Xp－ Tp和 Xp— Ap键，最后分解至单核苷酸和二核苷酸为止。这些核苷酸全都带有 3＇ -磷酸末端
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