脱氧核糖核酸酶I-03724778103

DNase I, recombinant, Grade I 03 724 778 103
from bovine pancreas, expressed in Pichia pastoris, lyo-
philizate
Recombinant produced DNase I in PCR grade, lyophilized quality, free of
animal-derived materials, is an essential tool for all applications requiring
DNase-free RNA templates.
Application
DNase I, recombinant, Grade I, is suitable for:
Isolation of DNA-free RNA produced by ■ in vitro transcription
Producing DNA-free preparations of protein and RNA: ■
To ensure that RT-PCR templates are free of genomic DNA ■
To remove DNA templates after ■ in vitro transcription of RNA
Nick-translation labeling of DNA with added DNA polymerase I ■
Determining the "footprint" of a DNA-binding protein ■
Microarray analysis ■
Benefits
Be compliant with regulatory requirements. ■ Assure that your ap-
plications are free of animal-derived materials.
Achieve reliable results. ■ Experience excellent quality and greater lot-
to-lot consistency due to advanced production processes in conjunction
with rigorous analytical testing.
Product Description
DNase I, recombinant, Grade I, originally isolated from bovine pancreas, is
a recombinant enzyme expressed in Pichia pastoris. It is a glycoprotein of a
molecular weight of approximately 39 kD. DNase I, recombinant, Grade I, is
a DNA-specific endonuclease that hydrolyzes phosphodiester linkages of
double- and single-stranded DNA to a mixture of mono- and oligonucleotides.
DNase I, recombinant, Grade I, is manufactured using state-of-the-art pro-
cesses yielding animal-free material.
EC 3.1.21.1
Properties
Nomenclature: DNase I
pH optimum: 7.0-8.0
Activators: DNase I requires bivalent cations for maximal activity.
Inhibitors: EDTA, EGTA, SDS
Specificity: Double-strand specific endonuclease that degrades DNA
Specification
Appearance: White to slightly yellowish lyophilizate
Activity (calf thymus DNA): ≥10 kU/vial lyophilizate
Activity (calf thymus DNA, modified buffer system): No limit
Unit definition: One unit according to Kunitz produces an increase in absor-
bance of 0.001/minute under assay conditions in 1 ml at 260 nm.
Proteases (resorufin-marked casein): Not detectable in up to 50 U after 17
hours incubation at +37°C.
Ribonucleases (MS2 RNA): Not detectable in up to 2 U after 4 hours incuba-
tion at +37°C.
Stability: At +2 to +8°C within specification range for 12 months.