胆固醇氧化酶-10129054103

Cholesterol Oxidase
from Nocardia erythropolis, lyophilizate 10 129 054 103
Oxidoreductase that catalyzes the interconversion of cholesterol to cholest-4-
en-3-one.
Application
Use Cholesterol Oxidase in diagnostic tests for the determination of choles-
terol in combination with Cholesterol Esterase, Catalog Nos. 10 129 046 103, 10
262 609 103, 11 015 923 103, 11 520 857 103 or 11 641 735 103.
Benefits
Rely on the proven diagnostic quality of this product.
EC 1.1.3.6
Properties
Nomenclature: Cholesterol:oxygen oxidoreductase
Molecular weight: ~59 kD
Isoelectric point: 4.85
Michaelis constants (Cholesterol):
Phosphate buffer, pH 7.0: 1 x 10 -6 mol/l
Triton X-100/isopropanol, pH 7.0: 7 x 10 -6 mol/l
Inhibitors: ZnCl 2 , Brij 35, Tween 40, Tween 60, Hg 2+ , GSH, sodium dodecyl
sulfate,
laurylbenzenesulfonate
Activators: Hydroxypolyethoxydodecane, Triton X-100, DOC
pH optimum: 7.0-8.0 (see figure)
Temperature dependence: See figure
pH stability: 5.5-6.5 (see figure)
Thermal stability: Up to +50°C (see figure)
Specificity: Cholesterol (100%), b-cholestanol, pregnenolone, b-sitosterol and
stigmasterol react as substrates (65-70%). Dehydroisoandrosterone and ergos-
terol show 5% relative activity. Androsterone, testosterone, β-estradiol, vitamin
D3, cholic acid and cholesterol acetate do not react.
Specification
Appearance: Yellow-brown lyophilizate
Solubility: Clear, yellowish solution in water (c=10 mg/ml)
pH value (c=10 mg/ml in water): 5.5-6.5
Activity (+25°C, cholesterol): ≥2.5 U/mg lyophilizate
Protein (Lowry): 0.05-0.12 mg/mg lyophilizate
Contaminants (expressed as percentage of Cholesterol Oxidase activity):
Catalase: ≤6.0
Cholesterol esterase: No limit
Glucose oxidase: ≤0.01
"NADH oxidase": ≤0.01
Uricase: ≤0.01
Stability: At -15 to -25°C within specification range for 15 months. Store dry.