胆固醇氧化酶-11479709103

Cholesterol Oxidase 胆固醇氧化酶-11479709103
from Brevibacterium species, expressed in E.coli, lyo-
philizate
Oxidoreductase that catalyzes the interconversion of cholesterol to cholest-4-
en-3-one.
Application
Use Cholesterol Oxidase in diagnostic tests for the determination of choles-
terol in combination with Cholesterol Esterase, Catalog Nos. 10 129 046 103, 10
262 609 103, 11 015 923 103, 11 520 857 103 or 11 641 735 103.
Benefits
Rely on the proven diagnostic quality of this product.
EC 1.1.3.6
Properties
Nomenclature: Cholesterol:oxygen oxidoreductase
Molecular weight: 60 kD (native and SDS)
Isoelectric point: ~5.0
Michaelis constant (Phosphate buffer, 0.5 mol/l, pH 7.5; +25°C):
Cholesterol: 1 x 10 -4 mol/l
Inhibitors: Hg 2+ , ZnCl 2 , SDS
Activators: Non ionic detergents
pH optimum: 5.5-8.0 (see figure)
Temperature dependence: See figure
pH stability: 5.0-10.0 (see figure)
Thermal stability: Up to +55°C (see figure)
Storage and Stability: No decrease in activity over 6 weeks at +35°C (see
figure)
Specificity:
cholesterol 100%
pregnenolon 52%
stigmasterol 17%
dehydroandrosterone 0.5%
androsterone 0%
estradiol 0%
cholate 0%
Specification
Appearance: Yellow lyophilizate
Solubility: Clear, yellowish solution in water (c=10 mg/ml)
pH value: 6.0-7.0
Protein (Biuret): 0.1-0.3 mg/mg lyophilizate
Activity (+25°C, cholesterol): 10-20 U/mg lyophilizate
Contaminants (expressed as percentage of Cholesterol Oxidase activity):
Catalase: ≤6.0
Glucose oxidase: ≤0.01
"NADH oxidase": ≤0.01
Uricase: ≤0.01
Stability: At -15 to -25°C within specification range for 12 months. Store dry.
Protect from light.