Fluorescein-12-dUTP is used as a substrate for: • terminal transferase • DNA polymerase I (holoenzyme and Klenow fragment), • Taq DNA polymerase • reverse transcriptase (e.g., from AMV and M-MuLV). Fluorescein-12-dUTP has been used to label nuclear extracts during PCR. It has also been used to replace dTTP in the random-primed DNA labeling reaction or in nick translation reactions, as well as in PCR. The nucleotide also serves as a substrate for terminal transferase in 3′-end labeling and TUNEL. Fluorescein-labeled probes can be used for in in situ hybridization with direct fluorescence detectionor detection by enzyme linked immunoimmunsorbent assay (ELISA) using Anti-Fluorescein-AP, Fab fragments. Repeated fluorescence labeling using Tetramethyl-Rhodamine-6-dUTP (red) and AMCA-6-dUTP (bright blue) is possible.
Quality
典型分析:至少85%荧光素-12-dUTP(HPLC,峰面积%)。
Physical form
荧光素-12-dUTP,四锂盐。
Storage and Stability
The unopened reagent is stable at 15 to 25°C until the expiration date printed on the label. Decomposition of approx. 5% may occur within 6 months.