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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
GC-RICH PCR System, dNTPack
- 保质期：
见产品外包装
- 供应商：
嵘崴达
- 保存条件：
−20°C
- 库存：
需确认
- 规格：
50 reactions
GC-RICH PCR System, dNTPack
General description
The GC-RICH PCR System, dNTPack is composed of a special enzyme blend of thermostable Taq DNA Polymerase and Tgo DNA Polymerase, a thermostable enzyme with a proofreading (3′-5′ exonuclease) activity. This polymerase mixture by itself outperforms Taq DNA Polymerase in respect to yields, fidelity and specificity beside the possibility to amplify fragments up to 5 kb in length. The GC-RICH PCR reaction buffer in combination with the included GC-RICH resolution solution allows to amplify very efficiently difficult templates like GC-rich targets.
Application
The GC-RICH PCR System, a blend of Taq DNA Polymerase and a proofreading polymerase, enables amplification of templates that are difficult or impossible to amplify with other polymerases and other blends of polymerases. The enhanced processivity of the blend and the unique GC-RICH Resolution Solution combine to deliver superior performance – especially from problematic templates.
The GC-RICH PCR System, dNTPack may also be used in standard PCR applications, providing improved results (higher yield, higher accuracy) over Taq DNA Polymerase alone.
Features and Benefits
• Ease access to difficult templates:-
including GC-rich targets and repetitive sequences.
• Reagents, the GC-RICH Resolution Solution and PCR Grade Water are provided.
• Amplify DNA fragments up to 5 kb.
• Cost-effective:-
use the convenient premixed solution of PCR grade NTPs.
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文献和实验An Economic PCR Enhancer for GC-Rich PCR Templates
Since PCR is often problematic on GC-rich templates, we have evaluated different PCR enhancing additives and generated an Combinatorial Enhancer Solution (CES). We tested this solution on approx. 50 different primer pairs using
Minimum GC-Rich Sequences for Overlap Extension PCR and Primer Annealing
, the annealing sequences can be shortened. Here, we describe universal GC-rich annealing sequences useful for overlap extension PCR and simple in-frame addition of desired sequences.
GC-Rich Template Amplification by Inverse PCR: DNA Polymerase and Solvent Effects
The amplification of GC-rich templates by any PCR method is usually a difficult task and despite the development of modified methods and conditions, this type of amplification still remains a specific case approach. Problems usually observed
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