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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Bovine Serum Albumin Fraction V
- CAS号:
见产品外包装
- 保质期:
见产品外包装
- 供应商:
嵘崴达
- 保存条件:
见产品外包装
- 库存:
需确认
- 规格:
50 g
Bovine Serum Albumin Fraction V
from bovine serum
| packaging | pkg of 1 kg (10735108001) |
| pkg of 100 g (10735086001) | |
| pkg of 50 g (10735078001) | |
| pkg of 500 g (10735094001) |
General description
For standard applications.
Application
Bovine Serum Albumin Fraction V is suitable for standard applications such as:
• Stabilizer to prevent proteolysis and denaturation of enzymes and other proteins, especially when the proteins are present in low concentrations.
• Buffer component in immunochemistry, biochemistry, cell biology, or molecular biology
• Reducing agent
• Protein blocker for saturation of protein binding sites in ELISAs, Southern blots, and western blots
• Media supplement for media that require addition of protein
• Carrier protein
• Standard protein in gel electrophoresis and protein determination
Quality
Purity: ≤95% protein, ≤5% H2O; electrophoretically homogeneous
Contaminants: <0.5% Na (flame photometric), <0.006% K (flame photometric), <0.0003% Li (flame photometric), <0.02% Ca (o-cresolphthalein), <0.003% Mg (xylidyl blue), <0.003% heavy metals (as Pb), <0.001% Fe (bathophenanthroline), <0.002% Pi, <0.05% glucose (enzymatic), <0.005% glycerol (enzymatic), <0.025% L-lactate (enzymatic)
Preparation Note
Storage conditions (working solution): In solution, albumin is stable for about three months at -15 to -25 °C.
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文献和实验Isolation of Microtubules (Bovine Brain)
minutes at 4° C for further clarification. Collect 10 ml of the supernatant and determine the protein content of your sample using the Bradford protein assay (Appendix G ). Use bovine serum albumin or lysozyme for establishing a standard
Culture of BEND Cells (Bovine Endometrial Cells)
Hams F-12 (N6760) Powdered MEM D-valine modification (M7395) Fetal bovine serum (F4135) Horse serum (H1138) Antibiotic-antimycotic (ABAM) (A9909) D-valine (V1255) Insulin (I5500) Preparation 1. Place 1000 ml of double distilled water
Primary Cultures of Astrocytes from Fetal Bovine Brain
We describe here a method to obtain primary cell cultures from the cerebral cortex and the hypothalamus of bovine fetuses. We report how tissue origin, developmental stages, and culture medium conditions influence cell
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