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- 2025年07月16日
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文献和实验). 3. Tris-buffered saline (TBS) with Tween (TBS-T): Prepare10× stock with 1.37 M NaCl, 27 mM KCl, 250 mM Tris-HCl, pH 7.4, and 1% (v/v) Tween-20. 4. Blocking buffer: 4% (w/v) bovine serum albumin (BSA) and0.1% (w/v) sodium azide in TBS. 5. Primary
: L-Wnt3A (ATCC , Manassas, VA; ATCC# CRL-2647) (see Note 1 ). 2. Cell culture medium: Dulbecco’s minimum essential medium(DMEM) supplemented with 10% (v/v) fetal bovine serum(FBS), 1:100 dilution of penicillin–streptomycin solution with10,000 U
Procedures for In Vitro Production of Bovine Embryos
(E2758) BME amino acids solution (B6766) Bovine serum albumin, Fraction V (A3311) Bovine serum albumin, essentially fatty acid free (A6003) Calcium chloride (C7902) Epinephrine (E4250) DL-Lactic acid, sodium salt, 98% syrup (L-
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