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348
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长期
- 供应商:
北京百奥莱博科技有限公司
特别声明:本产品及我公司所售其他产品均为科研类试剂产品,严禁用于药物、医疗及其他非科研用途。
北京百奥莱博专业生产供应JN0034型2×Complex Taq PCR MasterMix哪里卖,我公司销*(代"售")全品类的分子生物学试剂,价格优惠,欢迎广大科研工作者垂询订购。
名称:JN0034型2×Complex Taq PCR MasterMix哪里卖
编号:JN0034
品牌:百奥莱博
规格:1ml×5
本制品是由Taq DNA 聚合酶、dNTP混合物、MgCl2以及反应缓冲液 预先配制成2倍浓度的混合物,是JN0030 2×Taq PCR MasterMix同一系列 产品,其配方专门针对复杂模板扩增条件进行优化,具有更好的稳定 性,更好的扩增特异性,可减少非特异性条带的扩增,能提高复杂模 板及高GC含量模板的扩增效果。使用时,只需在加入模板和引物并稀 释到1倍浓度即可进行PCR反应,大大地简化了操作过程,减少了PCR操 作过程中的污染。2×Complex Taq PCR MasterMix提供普通型/快速上 样型(含蓝色染料)两种形式供您选择。经测试,高纯度染料的加入 不影响PCR反应,在PCR反应完成后可直接电泳,节省时间。
产品特点:
高效:以DNA为模板,扩增长度可达8kb,能高效扩增4kb以下片 段。
稳定:反复冻融五十次,4℃放置30天,室温放置一周后,扩增性能不受影响。
快捷:PCR反应所必需试剂全集于一管之中,数分钟即可完成反应 体系配制。
便利:10μl, 25μl 或50μl体系全部适用。PCR反应后可直接电泳(含染料形式)。
产品用途:
1、适用于常规PCR鉴定及较短DNA片段的PCR克隆;
2、复杂模板扩增,高GC含量模板扩增;
3、平端PCR产物加A。
应用实例:

图例一)在50μl扩增体系中,分别以人基因组DNA为模板,对特定基因片段 (1000bp) 进行扩增。
泳道1,2:2×Complex Taq PCR MasterMix;泳道3,4:A公司的Taq预混液;泳道M:DNA Ladder 2000
常用反应体系(50μl):
| 组分 | 体积 |
| 2×PCR MasterMix* | 25μl |
| 上游引物 | 0.2-1.0μM(终浓度) |
| 下游引物 | 0.2-1.0μM(终浓度) |
| 模板 | 1-50ng(质粒) |
| 10ng-1μg(基因组) | |
| ddH2O | 至50μl |
| *Mg2+终浓度为2mM | |
常用PCR循环:
| 当扩增片段<3K: | |
| 94℃ | 1分钟30秒 |
| 30次循环 | 94℃,20秒 57℃,20秒 72℃,根据产物长度调整,60秒/kb |
| 72℃ | 5分钟 |
| 4℃ | 保温 |
| 当扩增片段≥3K(推荐引物长度≥30bp): | |
| 94℃ | 20秒 |
| 30次循环 | 94℃,5秒 68℃,根据产物长度调整,60秒/kb |
| 72℃ | 5分钟 |
| 4℃ | 保温 |
PCR MasterMix选购指南:
| 编号 | 产品名称 | 特点 |
| 适用于菌落鉴定、菌液鉴定及常规PCR鉴定实验的Mix系列 | ||
| JN0030 | 2×Taq PCR MasterMix | 高性价比PCR Mix,使用简便,扩增灵敏度高 |
| JN0034 | 2×Complex Taq PCR MasterMix | JN0030改进版,性能更均衡,适用面更广 |
| JN0040 | 2×Fast Taq PCR MasterMix | 最快速的PCR试剂,常规PCR鉴定45分钟内知道结果 |
| JN0035 | 2×RoomTop Taq PCR MasterMix | 可放在手边的PCR试剂,室温稳定存放60天 |
| JN0036 | 2×RoomTop Fast Taq PCR MasterMix | 室温稳定存放60天,高速PCR试剂,常规PCR鉴定45分钟内知道结果 |
| 适用于一些较难扩增的PCR条带的鉴定及短片段克隆 | ||
| JN0032 | 2×Taq Plus PCR MasterMix | 扩增效率更高及较好的保真度 |
| JN0038 | 2×Hot Start Taq PCR MasterMix | 采用热启动Taq,可获得很好的特异性 |
| JN0041 | 2×Ultra Taq PCR MasterMix | 高速扩增酶,优化配方,解决PCR扩增难题的,灵敏度高 |
| 高保真酶试剂,适用于长片段基因克隆 | ||
| JN0031 | 2×Pfu PCR MasterMix | 采用高保真酶,适用于基因克隆 |
| JN0043 | 2×Fast Pfu PCR MasterMix | 采用改良型高保真酶,更好的扩增效果,4倍扩增速度;扩增迅速 |
| 防止PCR产物二次污染、极高检测灵敏度的PCR试剂 | ||
| JN0047 | 2×PCR MasterMix(防污染) | 采用dUTP/UDG酶系统,从根本上杜绝PCR产物二次污染 |
| JN0033 | 2×Genotyping PCR Kit(nomal) | 极高检测灵敏度,可达到普通PCR试剂102至104倍扩增灵敏度 |
PCR MasterMix性能对比表:
| 名称 | 编号 | 扩增时间 | 扩增长度 | 是否有3"-A | 基因组扩增 |
| 2×Taq PCR MasterMix | JN0030 | 1min/kb | ≤8kb | √ | + |
| 2×RoomTop Taq PCR MasterMix | JN0035 | 1min/kb | ≤8kb | √ | ++ |
| 2×Complex Taq PCR MasterMix | JN0034 | 1min/kb | ≤8kb | √ | + |
| 2×Fast Taq PCR MasterMix | JN0040 | 15s/kb | ≤8kb | √ | + |
| 2×RoomTop Fast Taq PCR MasterMix | JN0036 | 15s/kb | ≤8kb | √ | ++ |
| 2×Ultra Taq PCR MasterMix | JN0041 | 15s/kb | ≤8kb | √ | +++ |
| 2×Taq Plus PCR MasterMix | JN0032 | 1min/kb | ≤20kb | √ | ++++ |
| 2×Ultra Taq Plus PCR MasterMix | JN0042 | 15s/kb | ≤30kb | √ | ++++ |
| 2×Hot Start Taq PCR MasterMix | JN0038 | 1min/kb | ≤8kb | √ | ++++ |
| 2×Hot Start Taq Plus PCR MasterMix | JN0039 | 1min/kb | ≤20kb | √ | ++++ |
| 2×Pfu PCR MasterMix | JN0031 | 1min/kb | ≤6kb | × | + |
| 2×Fast Pfu PCR MasterMix | JN0043 | 30min/kb | ≤12kb | × | + |
| 2×SYBR Green Realtime PCR MasterMix | JN0049 | 1min/kb | ≤8kb | √ | ++ |
| 2×Genotyping PCR Kit(Normal) | JN0033 | 1min/kb | ≤8kb | √ | +++++ |
| 2×Genotyping PCR Kit(Fast) | JN0044 | 15s/kb | ≤8kb | √ | +++++ |
| 2×PCR MasterMix(防污染) | JN0047 | 1min/kb | ≤8kb | √ | + |
除JN0034型2×Complex Taq PCR MasterMix哪里卖外,我公司正在打折促销以下产品:
·重组小鼠IFN-gamma R1(CD119)(C端带Fc标签)
编号:JN1412
英文名称:Recombinant Mouse Interferon gamma Receptor 1
规格:10μg|50μg|500μg|1mg
本品由我们的哺乳动物细胞表达系统制备而成,目的基因编码的Ala26-Asp253在C端带有Fc标签。
IFN-gamma R1/CD119质量控制:>95%(还原性SDS-PAGE)
IFN-gamma R1/CD119制剂:冻干品
IFN-gamma R1/CD119保存:
冻干蛋白置于-20℃以下可长期保存,室温条件下可稳定保存3周。
复溶蛋白溶液可在4~7℃保存2~7天,可分装后置于-20℃保存三个月。
IFN-gamma R1/CD119复溶:
打开试剂管前请先离心。
复溶浓度推荐大于100 μg/ml。
冻干蛋白请溶于ddH2O。
复溶后,请根据用量分装冻存,避免反复冻融。
关于IFN-gamma R1/CD119:
The tetrameric receptor complex for IFNγ consists of two subunits, IFNGR1 (IFNγ Rα) and IFNGR2 (IFNγ Rβ), through which the dimeric IFN-γ exerts its biological functions, including antiviral, antiproliferation and immune-modulatory activity in mammals. Both IFNGR1 and IFNGR2 are single transmembrane proteins belonging to the class II cytokine family. FNGR1, widely expressed in most host cells, is essential for IFNγ binding, receptor trafficking, and signal transduction. IFNGR1 possesses an intracellular Janus tyrosine kinase (JAK) 1 binding site, a signal transducer and activator of transcription 1 (STAT1) binding site. The resulting STAT1 homodimers translocate from the cytoplasm to the nucleus and bind to the interferon-gamma activated sequence (GAS) promoter to induce expression of downstream interferon stimulated genes (ISGs).
JN0034型2×Complex Taq PCR MasterMix哪里卖关键词:2×Complex Taq PCR MasterMix,JN0034,百奥莱博
·重组人干扰素α(βR2(IFN-α)(βR2))
编号:JN0424
英文名称:Recombinant Human Interferon alpha/beta Receptor 2
规格:10μg|50μg|500μg|1mg
本品由我们的哺乳动物细胞表达系统制备而成,目的基因编码的Ile27-Lys243在C端含有His标签。
IFN-alpha/beta R2质量控制:>95%(还原性SDS-PAGE)
IFN-alpha/beta R2制剂:冻干品
IFN-alpha/beta R2保存:
冻干蛋白置于-20℃以下可长期保存,室温条件下可稳定保存3周。
复溶蛋白溶液可在4~7℃保存2~7天,可分装后置于-20℃保存三个月。
IFN-alpha/beta R2复溶:
打开试剂管前请先离心。
复溶浓度推荐大于100 μg/ml。
冻干蛋白请溶于ddH2O。
复溶后,请根据用量分装冻存,避免反复冻融。
关于IFN-alpha/beta R2:
Interferon α/β Receptor 2 (IFN-α/β R2) is a single-pass type I membrane protein which belongs to the type II cytokine receptor family. It complexes with IFN-α/β R1 to form the signaling receptor complex for the family of α and β IFN subtypes. By alternative splicing, IFN-α/β R2 can exist as a secreted soluble protein or as a type I membrane protein. IFN-α/β R2 is the principal ligand binding subunit of the receptor. Ligand binding is stabilized by the subsequent association with IFN-α/β R1, resulting in the formation of a signaling ternary receptor complex. IFNAR2 was detected in most lymphocytes, monocytes, and granulocytes, although IFNAR2 expression was higher in the monocytes and granulocytes than in the lymphocytes. Among the lymphocyte subsets, IFNAR2 showed high expression in natural killer (NK) cells and low expression in T lymphocytes. Isoform 1 and isoform 3 of IFNAR2 are directly involved in signal transduction due to their interaction with the TYR kinase, JAK1. Isoform 1 also interacts with the transcriptional factors, STAT1 and STAT2. Both forms are potent inhibitors of type I IFN activity.
JN0034型2×Complex Taq PCR MasterMix哪里卖关键词:2×Complex Taq PCR MasterMix,JN0034,百奥莱博
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北京百莱博科技有限公司专业生产供应销*(代"售")生物试剂、化学试剂、抗原抗体、血清血浆、Elisa试剂盒,更多试剂产品需求,欢迎来电咨询选购JN0034型2×Complex Taq PCR MasterMix哪里卖。
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文献和实验for both DNA sample and reaction mixture preparation, is strongly recommended.The reagents for PCR should be prepared separately and used solely for this purpose. Autoclaving of all solutions, except dNTPs, primers and Taq DNA Polymerase is recommended
bp 片段,以 pBS 菌液为模板扩增 1500 bp 片段。 模板:菌液 OD600=0.8 时 2 μL /50 μL PCR 扩增程序:同上 a 扩增程序同。 结果:Bioteke 2×power Taq PCR MasterMix 试剂对于质粒或菌液均可高效率的扩增。取扩增产物 10 μL 用 1% 琼脂糖凝胶电泳进行分析可见明亮条带。 二、genomic DNA扩增 1.大肠杆菌DNA扩增 模板:大肠杆菌DNA 50 ng/50 μL PCR扩增程序: 循环数 Step 温度
Cloning of Taq polymerase-amplified PCR products
" vector) Taq polymerase has a nontemplate-dependent terminal transferase activity that adds a single deoxyadenosine (A) to the 3´ ends of PCR products. The linearized vector supplied in this kit has single, overhanging 3´ deoxythymidine (T) residues
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