- ¥400
- EASYBIO
- BE7003-10
- 2025年12月24日
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- 克隆性：
多克隆
- 规格：
10ul/支
flag-HRP Mouse Monoclonal Antibody
The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.
Product Name: Flag Mouse Monoclonal Antibody-HRP conjugated
Isotype: IgG1
Clone Number: 3C6
Storage Buffer : PBS, pH 7.4, containing 0.02% kathon as Preservative and 50% Glycerol
Storage instructions: -20°C. Do not aliquot the antibody
Recommended dilutions: WB: 1:2,000-5,000
Optimal dilutions should be determined by the end user.
Specificity: The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion proteins.
Alternative Names: DDDDK epitope tag, DYKDDDDK epitope tag, ECS epitope tag
Form: Liquid
Reactivity: ALL
Western blot analysis of Recombinant protein with BE7003 diluted at
1) 1:2,000
2) 1:5,000
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文献和实验Conjugation of Antibody to HRP
of Maleimide:HRP Weigh out 1.067 mg HRP for each mg of thiolated antibody. Add 1.1 mg Sulfo-SMCC per mg of HRP Add PBS to a final concentration of 8 mg/ml HRP Mix for 2 hours on a rotator. Desalt the HRP on PBS equilibrated column. Collect
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
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