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- 2026年04月05日
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- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
多克隆
- 抗体英文名：
C-Myc-Tag Mouse Monoclonal Antibody
- 规格：
支
c-Myc-tag antibody is part of the Tag series of antibodies, the best quality in the research. The immunogen of c-Myc tag antibody is a synthetic peptide corresponding to residues 410-419 of the human p62 c-myc protein conjugated to KLH. C-Myc tag antibody is suitable for detecting the expression level of c-Myc or its fusion proteins where the c-Myc tag is terminal or internal.
Product Name:c-Myc-tag mouse monoclonal antibody
Clone Number: 4E9
Isotype: IgG1
Immunogen: A synthetic peptide EQKLISEEDLcoupled to KLH.
Tested applications and dilutions:WB 1:5000-2000 IP 1:200 IF 1:2000
Produce anti- c-myc antibody affinity column purify c-myc recombinant proteins
Form:Liquid
Concentration: 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol
Storage: c-Myc antibody is stable for 1 month at 2-8°C. For long term storage, Aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
A: HEK 293 cell lysate without transfected
B: HEK 293 cell lysate transfected with c-myc-tag protein
C: IP (PBS + anti- c-myc mAb 4℃overnight)
D: IP (HEK293 transfected+protein G+anti- c-myc mAb 4℃overnight)
E: IP (HEK293 transfected+RIPA+Protein G 4℃overnight)
F: Multi Tag Recombinant protein (expressed in E.coli)
TP=Target Protein HC=Heavy chain LC=Light Chain
IF analysis of 293T cells transfected with a MYC-tagged protein, using easybio MYC-Tag (4E9) Mouse mAb at a 1:2000 dilution (blue DAPI ,red anti-C-MYC)
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文献和实验Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma，在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应，对应于抗原之不同部分可制成各种不同的抗体复合体。然而，因为一个产生抗体的细胞，只能产生一种抗体，所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种，可形成边产生抗体边增殖的 hybridoma，将每个杂种细胞进行克隆（无性繁殖系）培养，则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成，这样得到的就是单克隆抗体。此抗体在测定
Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
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