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- EASYBIO
- BE3010-100
- 2026年01月02日
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- 详细信息
- 文献和实验
- 技术资料
- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
多克隆
- 抗体英文名：
Histone H3(mono methyl K79) Mouse Monoclonal Antibody
- 规格：
支
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
Product Name:Histone H3 (mono methyl K79)Mouse Monoclonal Antibody
Clone Number: 5C10
Isotype: IgG1
Storage Buffer :PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol
Storage instructions:-20°C. Do not aliquot the antibody
Recommended dilutions: WB: 1:500-2,000
Optimal dilutions should be determined by the end user.
Specificity: Antibody can detects endogenous Histone H3 (mono methyl K79) protein.
Alternative Names:H3 histone antibody, HIST1H3A antibody, Histone cluster 1, H3a antibody
Molecular Weight:15KD
Form: Liquid
Reactivity:H, R, M
Western blot analysis of 1) Hela, 2) Rat Testis tissue, 3) Raw264.7 with Histone H3 (mono methyl K79) (5C10) Mouse MAb diluted at 1:2000.
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文献和实验Determination of Histone Methylation in Mono- and Dicotyledonous Plants
. It has been found that the methylation pattern in the histone H3 may provide understanding of the process involved in cell differentiation, adaptation, and evolution in plants. In this work, we detail a method for isolation of nuclear proteins from small amount of sample to identify
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Detection of Histone H3 Phosphorylation in Cultured Cells and Tissue Sections by Immunostaining
. The protocol described here allows the detection of phosphorylated histones in tissue-cultured cells and tissue sections by fluorescent or bright-field immunostaining analysis. Here we used a serine 10 specific P-histone H3 antibody to determine
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