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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 抗体名:
CD41/CD61 (PAC-1 epitope) Antibody (FITC) 抗体
- 抗体英文名:
CD41/CD61 (PAC-1 epitope) Antibody (FITC)
- 靶点:
CD41/CD61 (PAC-1 epitope)
- 应用范围:
FC
- 适应物种:
Human
- 保质期:
12 months from date of receipt.
- 抗原来源:
详询
- 目录编号:
orb414541
- 级别:
科研
- 库存:
99
- 供应商:
Biorbyt
- 标记物:
FITC
- 克隆性:
Monoclonal
- 保存条件:
详询
- 亚型:
Mouse IgM kappa
- 免疫原:
详询
- 规格:
100 T
产品子类型: Primary Antibody
描述: Mouse monoclonal antibody against PAC-1 conjugated to FITC
克隆号: PAC-1
别名: platelet GPIIb, Integrin alpha-Iib, GPalpha IIb, GPIIb, GT, GP3A, platelet GPIIIa
应用说明: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
免疫原: Human platelets
防腐剂: Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
纯化方法: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
特异性: The mouse monoclonal antibody PAC-1 recognizes an extracellular activation-induced conformational epitope PAC-1 on CD41/CD61 complex (gpIIb/IIIa), also known as integrin alpha IIb beta 3, a receptor which mediates platelet aggregation.
研究领域: Neuroscience; Immunology & Inflammation
Entrez 基因 ID: 35
保存条件: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
备注: For research use only.

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文献和实验上发展起来的一种多参数分析,可以更全面、更系统的获得血小板相关信息。 血小板活化试剂组 (1)PAC-1-FITC抗体:IgM型,与活化的GPⅡb/Ⅲa复合物结合,是早期血小板活化标志物。RGDS为PAC-1FITC抗体结合阻断剂,作阴性对照。 (2)CD62P-PE抗体:IgG1型。血小板活化时表面的CD62P结合,是血小板活化后期的标志物。 3)CD61-PerCP:IgG1型。特异的泛血小板表面标记,既与活化血小板结合,也与未活化血小板结合。 3、操作步骤 标本采集(全血法)→血小
试剂组成为: PAC-1 FITC :早期血小板活化的标志物,即活化的 gp Ⅱ b/ Ⅲ a 复合物,抗体与活化后血小板膜表面的 gp Ⅱ b/ Ⅲ a 复合物纤维蛋白原结合位点相结合。 BDIS 抗体为 IgM 型,建议使用 PAC-1 结合阻断剂 RGDS 做阴性对照。 CD62P PE :血小板活化后期的标志物,特异地与活化血小板结合。静止血小板胞浆内 a 颗粒含有 CD62P ,血小板活化时,颗粒内糖蛋白向膜外释放
Flow Cytometry Assay to Measure Internalization of GPCRs
. However, the user can probably also substitute PBS Ca-free, Mg-free plus 0.04% EDTA to lift cells.7) Pellet cells in centrifuge set at 4degC and rinse 1x with media. NOTE: Since the anti-FLAG M1 antibody (Sigma, Covance)requires Ca ions to bind the FLAG epitope
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