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- 详细信息
- 技术资料
- 抗体名:
细胞质磷酸酶1抗体
- 抗体英文名:
Anti-Phospho1
- 靶点:
详见说明书
- 浓度:
1mg/1ml
- 应用范围:
WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
- 宿主:
详见说明书
- 供应商:
上海一研
- 库存:
34
- 级别:
详见说明书
- 目录编号:
详见说明书
- 抗原来源:
Rabbit
- 保质期:
详见说明书
- 适应物种:
详见说明书
- 标记物:
详见说明书
- 克隆性:
多克隆
- 保存条件:
低温冷藏
- 形态:
详见说明书
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived from human Phospho1
- 规格:
0.2ml/200μg
公司抗体应用于WB、IHC、IF、ELISA等实验中,细胞质磷酸酶1抗体品牌品质保证,提供完善的售前、售中、售后服务!欢迎来电咨询或联系在线客服!我们将竭诚为您服务!
英文名称 Anti-Phospho1
中文名称 细胞质磷酸酶1抗体品牌
别 名 EC 3.1.3.75; PHOP1_HUMAN; Phosphatase orphan 1; Phosphatase, orphan 1; Phospho 1; phospho1; Phosphoethanolamine/phosphocholine phosphatase.
浓 度 1mg/1ml
规 格 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit
产品类型 一抗
研究领域 细胞生物 发育生物学 信号转导 干细胞 激酶和磷酸酶
蛋白分子量 predicted molecular weight: 30kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human Phospho1
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 PHOSPHO1 is a 267 amino acid phosphatase that is a member of the haloacid dehalogenase (HAD) superfamily of magnesium-dependent hydrolases. PHOSPHO1 is highly expressed in bone and cartilage and localizes to the osteoid layer of the periosteum. PHOSPHO1 is restricted to sites of mineralization and its inhibition decreases the ability of matrix vesicles to calcify in bone, suggesting that the protein may play a role in the matrix mineralization process during skeletal development. PHOSPHO1 cleaves phosphoethanolamine and phosphocholine to generate inorganic phosphate for bone mineralization. PHOSPHO1 contains three catalytic motifs that are conserved within the haloacid dehalogenase superfamily.
Function : Phosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). Involved in the generation of inorganic phosphate for bone mineralization.
Tissue Specificity : Expressed at sites of mineralization in bone and cartilage. Highly expressed in osteoblast cell line SaOS-2 which produces a mineralized matrix, but not in MG-63 cell line, which do not mineralize.
Similarity : Belongs to the HAD-like hydrolase superfamily. PHOSPHO family.
Database links : UniProtKB/Swiss-Prot
细胞质磷酸酶1抗体品牌具有高度的特异性,免疫组织化学正是利用了这一原理。先将组织或细胞中的某种化学物质提取出来,以此作为抗原或半抗原,通过免疫动物后获得特异性的抗体,再以此抗体去探测组织或细胞中的同类的抗原物质。由于抗原与抗体的复合物是无色的,因此还必须借助于组织化学的方法将抗原抗体结合的部位显示出来,以其达到对组织或细胞中的未知抗原进行定性,定位或定量的研究。
细胞质磷酸酶1抗体品牌包括以下几个步骤:
1、制备抗原。
2、选择实验动物。
3、动物免疫。
4、试取血进行测试,看看是否成功免疫。
5、如果成功免疫,杀死实验动物,采集全部血清。
6、纯化出抗体。
7、鉴定抗体。包括纯度以及特异性。
细胞质磷酸酶1抗体品牌的功能
抗体的主要功能是与抗原(包括外来的和自身的)相结合,从而有效地清除侵入机体内的微生物、寄生虫等异物,抗体(antibody)是一种应答抗原产生的、可与抗原特异性结合的蛋白质。每种抗体与特定的抗原决定基结合。这种结合可以使抗原失活,也可能无效但有时也会对机体造成病理性损害,如抗核抗体、抗双链DNA抗体、抗甲状腺球蛋白抗体等一些自身抗体的产生,对人体可造成危害。
细胞质磷酸酶1抗体品牌的优点:
①耐热、耐酸、耐碱、抗有机溶剂以及金属离子,稳定性好,室温下可以长期保存;
②制备简单,操作方便,可以进行批量;
③不必免疫动物,且可获得免疫动物所不能得到的“抗体”;
④可以反复使用;
⑤价格低廉。因此,设计、合成既具有类似生物抗体的高亲合性和高专一性,又具有耐热、耐酸、耐碱且又可以长期稳定的人工抗体,在部分领域替代生物抗体以进行仿生分子识别,或者完成一些生物抗体所不能完成的工作,具有重要的科学意义,在化学、生命科学和环境科学等方面具有广阔的应用前景
公司专业供应各种进口、国产一抗及二抗,品牌有R&D、Santa Cruz、Bipec、Millipore等国际知名品牌,品种多达7000多种,由于产品的数量众多,产品的宿主、抗原来源及应用范围(WB、IH(F/P)、FC、ELISA、IP、IF等)有所不同。
DonkeyAnti-GuineapigIgG/AlexaFluor350AlexaFluor350标记的驴抗豚鼠IgG规格:0.1ml
OTOA/DFNB22耳聋、常染色体隐性遗传22抗体规格:0.2ml
Snf1lk/SIK1丝氨酸/苏氨酸蛋白激酶SIK1抗体规格:0.2ml
LAP18/Stathmin白病相关蛋白18/蛋白18抗体规格:0.1ml
TTC33TTC33蛋白抗体规格:0.2ml
GoatAnti-MouseIgM/PEPE标记的羊抗小鼠IgM规格:0.1ml
ANKRD20A1锚蛋白重复结构域蛋白20A1抗体规格:0.2ml
RabbitAnti-mouseIgG/HRP辣根过氧化物酶标记的兔抗小鼠IgG规格:0.1ml
Phospho-FLT3(Tyr591)磷酸化FMS样酪氨酸激酶3规格:0.1ml
PATZ1转录调节因子MAZR抗体规格:0.2ml
CEK神经酰胺激酶CEK抗体规格:0.2ml
IRF7干扰素调节因子7抗体规格:0.1ml
TNIKTRAF2和NCK激酶相互作用蛋白抗体规格:0.2ml-I-L-E-V-P-S-T Glu-Ile-Leu-Glu-Val-Pro-Ser-Thr
Fibronectin Type III Connecting Segment (1-25) Asp-Glu-Leu-Pro-Gln-Leu-Val-Thr-Leu-Pro-His-Pro-Asn-Leu-His-Gly-Pro-Glu-Ile-Leu-Asp-Val-Pro-Ser-Thr
GIP (3-42), human Glu-Gly-Thr-Phe-Ile-Ser-Asp-Tyr-Ser-Ile-Ala-Met-Asp-Lys-Ile-His-Gln-Gln-Asp-Phe-Val-Asn-Trp-Leu-Leu-Ala-Gln-Lys-Gly-Lys-Lys-Asn-Asp-Trp-Lys-His-Asn-Ile-Thr-Gln
GRPP (human) Arg-Ser-Leu-Gln-Asp-Thr-Glu-Glu-Lys-Ser-Arg-Ser-Phe-Ser-Ala-Ser-Gln-Ala-Asp-Pro-Leu-Ser-Asp-Pro-Asp-Gln-Met-Asn-Glu-Asp
细胞质磷酸酶1抗体品牌HIV-1 tat Protein (1-9) Met-Asp-Pro-Val-Asp-Pro-Asn-Ile-Glu
Influenza HA (204-212) Leu-Tyr-Gln-Asn-Val-Gly-Thr-Tyr-Val
Leptin Receptor Precursor Pro-Phe-Val-Lys-pTyr-Ala-Thr-Leu-Ile-Ser-Asn
Mas17 Ile-Asn-Leu-Lys-Ala-Lys-Ala-Ala-Leu-Ala-Lys-Lys-Leu-Leu-NH2
Murine CMV pp 89 (168-176) Tyr-Pro-His-Phe-Met-Pro-Thr-Asn-Leu
Neurotensin Pyr-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu
P60c-src Substrate II Ac-Ile-Tyr-Gly-Glu-Phe-NH2
Pep 4A Gly-Ser-Val-Val-Ile-Val-Gly-Arg-Ile-Ile-Leu-Ser-Gly-Arg-NH2
Preprogalanin 28-67, rat Thr-Lys-Glu-Lys-Arg-Gly-Trp-Thr-Leu-Asn-Ser-Ala-Gly-Tyr-Leu-Leu-Gly-Pro-His-Ala-Ile-Asp-Asn-His-Arg-Ser-Phe-Ser-Asp-Lys-His-Gly-Leu-Thr-Gly-Lys-Arg-Glu-Leu-Pro
Renin Inhibitor Peptide His-Pro-Phe-His-Leu-D-Leu-Val-Tyr-NH2
英文名称 Anti-Phospho1
中文名称 细胞质磷酸酶1抗体品牌
别 名 EC 3.1.3.75; PHOP1_HUMAN; Phosphatase orphan 1; Phosphatase, orphan 1; Phospho 1; phospho1; Phosphoethanolamine/phosphocholine phosphatase.
浓 度 1mg/1ml
规 格 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit
产品类型 一抗
研究领域 细胞生物 发育生物学 信号转导 干细胞 激酶和磷酸酶
蛋白分子量 predicted molecular weight: 30kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human Phospho1
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 PHOSPHO1 is a 267 amino acid phosphatase that is a member of the haloacid dehalogenase (HAD) superfamily of magnesium-dependent hydrolases. PHOSPHO1 is highly expressed in bone and cartilage and localizes to the osteoid layer of the periosteum. PHOSPHO1 is restricted to sites of mineralization and its inhibition decreases the ability of matrix vesicles to calcify in bone, suggesting that the protein may play a role in the matrix mineralization process during skeletal development. PHOSPHO1 cleaves phosphoethanolamine and phosphocholine to generate inorganic phosphate for bone mineralization. PHOSPHO1 contains three catalytic motifs that are conserved within the haloacid dehalogenase superfamily.
Function : Phosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). Involved in the generation of inorganic phosphate for bone mineralization.
Tissue Specificity : Expressed at sites of mineralization in bone and cartilage. Highly expressed in osteoblast cell line SaOS-2 which produces a mineralized matrix, but not in MG-63 cell line, which do not mineralize.
Similarity : Belongs to the HAD-like hydrolase superfamily. PHOSPHO family.
Database links : UniProtKB/Swiss-Prot
细胞质磷酸酶1抗体品牌具有高度的特异性,免疫组织化学正是利用了这一原理。先将组织或细胞中的某种化学物质提取出来,以此作为抗原或半抗原,通过免疫动物后获得特异性的抗体,再以此抗体去探测组织或细胞中的同类的抗原物质。由于抗原与抗体的复合物是无色的,因此还必须借助于组织化学的方法将抗原抗体结合的部位显示出来,以其达到对组织或细胞中的未知抗原进行定性,定位或定量的研究。
细胞质磷酸酶1抗体品牌包括以下几个步骤:
1、制备抗原。
2、选择实验动物。
3、动物免疫。
4、试取血进行测试,看看是否成功免疫。
5、如果成功免疫,杀死实验动物,采集全部血清。
6、纯化出抗体。
7、鉴定抗体。包括纯度以及特异性。
细胞质磷酸酶1抗体品牌的功能
抗体的主要功能是与抗原(包括外来的和自身的)相结合,从而有效地清除侵入机体内的微生物、寄生虫等异物,抗体(antibody)是一种应答抗原产生的、可与抗原特异性结合的蛋白质。每种抗体与特定的抗原决定基结合。这种结合可以使抗原失活,也可能无效但有时也会对机体造成病理性损害,如抗核抗体、抗双链DNA抗体、抗甲状腺球蛋白抗体等一些自身抗体的产生,对人体可造成危害。
细胞质磷酸酶1抗体品牌的优点:
①耐热、耐酸、耐碱、抗有机溶剂以及金属离子,稳定性好,室温下可以长期保存;
②制备简单,操作方便,可以进行批量;
③不必免疫动物,且可获得免疫动物所不能得到的“抗体”;
④可以反复使用;
⑤价格低廉。因此,设计、合成既具有类似生物抗体的高亲合性和高专一性,又具有耐热、耐酸、耐碱且又可以长期稳定的人工抗体,在部分领域替代生物抗体以进行仿生分子识别,或者完成一些生物抗体所不能完成的工作,具有重要的科学意义,在化学、生命科学和环境科学等方面具有广阔的应用前景
公司专业供应各种进口、国产一抗及二抗,品牌有R&D、Santa Cruz、Bipec、Millipore等国际知名品牌,品种多达7000多种,由于产品的数量众多,产品的宿主、抗原来源及应用范围(WB、IH(F/P)、FC、ELISA、IP、IF等)有所不同。
DonkeyAnti-GuineapigIgG/AlexaFluor350AlexaFluor350标记的驴抗豚鼠IgG规格:0.1ml
OTOA/DFNB22耳聋、常染色体隐性遗传22抗体规格:0.2ml
Snf1lk/SIK1丝氨酸/苏氨酸蛋白激酶SIK1抗体规格:0.2ml
LAP18/Stathmin白病相关蛋白18/蛋白18抗体规格:0.1ml
TTC33TTC33蛋白抗体规格:0.2ml
GoatAnti-MouseIgM/PEPE标记的羊抗小鼠IgM规格:0.1ml
ANKRD20A1锚蛋白重复结构域蛋白20A1抗体规格:0.2ml
RabbitAnti-mouseIgG/HRP辣根过氧化物酶标记的兔抗小鼠IgG规格:0.1ml
Phospho-FLT3(Tyr591)磷酸化FMS样酪氨酸激酶3规格:0.1ml
PATZ1转录调节因子MAZR抗体规格:0.2ml
CEK神经酰胺激酶CEK抗体规格:0.2ml
IRF7干扰素调节因子7抗体规格:0.1ml
TNIKTRAF2和NCK激酶相互作用蛋白抗体规格:0.2ml-I-L-E-V-P-S-T Glu-Ile-Leu-Glu-Val-Pro-Ser-Thr
Fibronectin Type III Connecting Segment (1-25) Asp-Glu-Leu-Pro-Gln-Leu-Val-Thr-Leu-Pro-His-Pro-Asn-Leu-His-Gly-Pro-Glu-Ile-Leu-Asp-Val-Pro-Ser-Thr
GIP (3-42), human Glu-Gly-Thr-Phe-Ile-Ser-Asp-Tyr-Ser-Ile-Ala-Met-Asp-Lys-Ile-His-Gln-Gln-Asp-Phe-Val-Asn-Trp-Leu-Leu-Ala-Gln-Lys-Gly-Lys-Lys-Asn-Asp-Trp-Lys-His-Asn-Ile-Thr-Gln
GRPP (human) Arg-Ser-Leu-Gln-Asp-Thr-Glu-Glu-Lys-Ser-Arg-Ser-Phe-Ser-Ala-Ser-Gln-Ala-Asp-Pro-Leu-Ser-Asp-Pro-Asp-Gln-Met-Asn-Glu-Asp
细胞质磷酸酶1抗体品牌HIV-1 tat Protein (1-9) Met-Asp-Pro-Val-Asp-Pro-Asn-Ile-Glu
Influenza HA (204-212) Leu-Tyr-Gln-Asn-Val-Gly-Thr-Tyr-Val
Leptin Receptor Precursor Pro-Phe-Val-Lys-pTyr-Ala-Thr-Leu-Ile-Ser-Asn
Mas17 Ile-Asn-Leu-Lys-Ala-Lys-Ala-Ala-Leu-Ala-Lys-Lys-Leu-Leu-NH2
Murine CMV pp 89 (168-176) Tyr-Pro-His-Phe-Met-Pro-Thr-Asn-Leu
Neurotensin Pyr-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu
P60c-src Substrate II Ac-Ile-Tyr-Gly-Glu-Phe-NH2
Pep 4A Gly-Ser-Val-Val-Ile-Val-Gly-Arg-Ile-Ile-Leu-Ser-Gly-Arg-NH2
Preprogalanin 28-67, rat Thr-Lys-Glu-Lys-Arg-Gly-Trp-Thr-Leu-Asn-Ser-Ala-Gly-Tyr-Leu-Leu-Gly-Pro-His-Ala-Ile-Asp-Asn-His-Arg-Ser-Phe-Ser-Asp-Lys-His-Gly-Leu-Thr-Gly-Lys-Arg-Glu-Leu-Pro
Renin Inhibitor Peptide His-Pro-Phe-His-Leu-D-Leu-Val-Tyr-NH2
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细胞质磷酸酶1抗体品牌
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