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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
mTOR相关调控蛋白抗体
- 抗体英文名：
Anti-Raptor
- 靶点：
详见说明书
- 浓度：
1mg/1ml
- 应用范围：
WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
- 宿主：
详见说明书
- 供应商：
上海一研
- 库存：
52
- 级别：
详见说明书
- 目录编号：
详见说明书
- 抗原来源：
Rabbit
- 保质期：
详见说明书
- 适应物种：
详见说明书
- 标记物：
详见说明书
- 克隆性：
多克隆
- 保存条件：
Store at -20 °C
- 形态：
详见说明书
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide derived from human Raptor
- 规格：
0.2ml/200μg
公司抗体应用于WB、IHC、IF、ELISA等实验中，mTOR相关调控蛋白抗体品牌品质保证，提供完善的售前、售中、售后服务！欢迎来电咨询或联系在线客服！我们将竭诚为您服务！
英文名称 Anti-Raptor
中文名称 mTOR相关调控蛋白抗体品牌
别 名 p150 target of rapamycin (TOR) scaffold protein containing WD repeats; P150 target of rapamycin (TOR)-scaffold protein; Regulatory Associated Protein of mTOR; KIAA1303; RPTOR_HUMAN; Regulatory-associated protein of mTOR; p150 target of rapamycin (TOR)-scaffold protein.
浓 度 1mg/1ml
规 格 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit
产品类型 一抗
研究领域 肿瘤 免疫学 信号转导 细胞凋亡 转录调节因子
蛋白分子量 predicted molecular weight: 147kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human Raptor
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 mTOR controls cell growth, in part by regulating p70 S6 kinase alpha (p70alpha) and eukaryotic initiation factor 4E binding protein 1 (4EBP1). Raptor is a 150 kDa mTOR binding protein that also binds 4EBP1 and p70alpha. The binding of Raptor to mTOR is necessary for the mTOR-catalyzed phosphorylation of 4EBP1 in vitro, and it strongly enhances the mTOR kinase activity toward p70alpha. Rapamycin or amino acid withdrawal increases, whereas insulin strongly inhibits, the recovery of 4EBP1 and raptor on 7-methyl-GTP Sepharose. Partial inhibition of raptor expression by RNA interference (RNAi) reduces mTOR-catalyzed 4EBP1 phosphorylation in vitro. RNAi of C. elegans raptor yields an array of phenotypes that closely resemble those produced by inactivation of Ce-TOR. Thus, raptor is an essential scaffold for the mTOR-catalyzed phosphorylation of 4EBP1 and mediates TOR action in vivo.
Function : Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation.
Subunit : Interacts with MTOR (By similarity). Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR and AKT1S1. mTORC1 binds to and is inhibited by FKBP12-rapamycin. Binds directly to 4EBP1 and RPS6KB1 independently of its association with MTOR. Binds preferentially to poorly or non-phosphorylated forms of EIF4EBP1, and this binding is critical to the ability of MTOR to catalyze phosphorylation. Forms a complex with MTOR under both leucine-rich and -poor conditions. Interacts with ULK1 in a nutrient-dependent manner; the interaction is reduced during starvation. Interacts (when phosphorylated by AMPK) with 14-3-3 protein, leading to inhibit its activity.
Subcellular Location : Cytoplasm. Lysosome. Note=Targeting to lysosomes depends on amino acid availability.
Tissue Specificity : Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta.
Post-translational modifications : In response to nutrient limitation, phosphorylated by AMPK; phosphorylation promotes interaction with 14-3-3 proteins, leading to negative regulation of the mTORC1 complex. In response to growth factors, phosphorylated at Ser-719, Ser-721 and Ser-722 by RPS6KA1; phosphorylation positively stimulates mTORC1 activity.
Similarity : Belongs to the WD repeat RAPTOR family.
Contains 7 WD repeats.
Database links : UniProtKB/Swiss-Prot:
mTOR相关调控蛋白抗体品牌具有高度的特异性，免疫组织化学正是利用了这一原理。先将组织或细胞中的某种化学物质提取出来，以此作为抗原或半抗原，通过免疫动物后获得特异性的抗体，再以此抗体去探测组织或细胞中的同类的抗原物质。由于抗原与抗体的复合物是无色的，因此还必须借助于组织化学的方法将抗原抗体结合的部位显示出来，以其达到对组织或细胞中的未知抗原进行定性，定位或定量的研究。
mTOR相关调控蛋白抗体品牌包括以下几个步骤：
1、制备抗原。
2、选择实验动物。
3、动物免疫。
4、试取血进行测试，看看是否成功免疫。
5、如果成功免疫，杀死实验动物，采集全部血清。
6、纯化出抗体。
7、鉴定抗体。包括纯度以及特异性。
mTOR相关调控蛋白抗体品牌的功能
抗体的主要功能是与抗原（包括外来的和自身的）相结合，从而有效地清除侵入机体内的微生物、寄生虫等异物，抗体（antibody）是一种应答抗原产生的、可与抗原特异性结合的蛋白质。每种抗体与特定的抗原决定基结合。这种结合可以使抗原失活，也可能无效但有时也会对机体造成病理性损害，如抗核抗体、抗双链DNA抗体、抗甲状腺球蛋白抗体等一些自身抗体的产生，对人体可造成危害。
mTOR相关调控蛋白抗体品牌的优点：
①耐热、耐酸、耐碱、抗有机溶剂以及金属离子，稳定性好，室温下可以长期保存；
②制备简单，操作方便，可以进行批量；
③不必免疫动物，且可获得免疫动物所不能得到的“抗体”；
④可以反复使用；
⑤价格低廉。因此，设计、合成既具有类似生物抗体的高亲合性和高专一性，又具有耐热、耐酸、耐碱且又可以长期稳定的人工抗体，在部分领域替代生物抗体以进行仿生分子识别，或者完成一些生物抗体所不能完成的工作，具有重要的科学意义，在化学、生命科学和环境科学等方面具有广阔的应用前景
公司专业供应各种进口、国产一抗及二抗，品牌有R&D、Santa Cruz、Bipec、Millipore等国际知名品牌，品种多达7000多种，由于产品的数量众多，产品的宿主、抗原来源及应用范围(WB、IH(F/P)、FC、ELISA、IP、IF等)有所不同。
phospho-HDAC1(Ser423+Ser421)磷酸化组蛋白去乙酰化酶1抗体规格:0.1ml
FRMD2/FRMPD1FRMD2蛋白抗体规格:0.2ml
TNFSF4/CD252瘤坏死因子配体超家族成员4抗体规格:0.1ml
LRRC59LRRC59蛋白抗体规格:0.2ml
MouseAnti-RabbitIgM/HRP辣根过氧化物酶标记的小鼠抗兔IgM规格:0.1ml
DCP异常凝酶原/脱-γ-羧基凝酶原抗体规格:0.2ml
CARD17/INCA凋亡加强结构域蛋白17抗体规格:0.2ml
PACAPR1苷酸环化酶激活肽受体1抗体规格:0.1ml
phospho-MST1R(Tyr1353)磷酸化原基因c-Met相关酪氨酸激酶抗体规格:0.1mlBNP-26 (porcine) Asp-Ser-Gly-Cys-Phe-Gly-Arg-Arg-Leu-Asp-Arg-Ile-Gly-Ser-Leu-Ser-Gly-Leu-Gly-Cys-Asn-Val-Leu-Arg-Arg-Tyr (Disulfide bridge:Cys4-Cys5)
Calcineurin Substrate Asp-Leu-Asp-Val-Pro-Ile-Pro-Gly-Arg-Phe-Asp-Arg-Arg-Val-Ser-Val-Ala-Ala-Glu
Cecropin B, Free Acid Lys-Trp-Lys-Val-Phe-Lys-Lys-Ile-Glu-Lys-Met-Gly-Arg-Asn-Ile-Arg-Asn-Gly-Ile-Val-Lys-Ala-Gly-Pro-Ala-Ile-Ala-Val-Leu-Gly-Glu-Ala-Lys-Ala-Leu
mTOR相关调控蛋白抗体品牌Crustacean Cardioactive Peptide, CCAP Pro-Phe-Cys-Asn-Ala-Phe-Thr-Gly-Cys(Disulfide bridge Cys3-Cys9)
Dynorphin A (1-8), porcine Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile
Exendin 4 (3-39) Glu-Gly-Thr-Phe-Thr-Ser-Asp-Leu-Ser-Lys-Gln-Met-Glu-Glu-Glu-Ala-Val-Arg-Leu-Phe-Ile-Glu-Trp-Leu-Lys-Asn-Gly-Gly-Pro-Ser-Ser-Gly-Ala-Pro-Pro-Pro-Ser-NH2
Gastrin I (1-14) (human) Pyr-Gly-Pro-Trp-Leu-Glu-Glu-Glu-Glu-Glu-Ala-Tyr-Gly-Trp
gp100 (619-627) Arg-Leu-Met-Lys-Gln-Asp-Phe-Ser-Val
Hexa His His-His-His-His-His-His
Hypertrehalosaemic Neuropeptide, Nauphoeta cinerea Pyr-Val-Asn-Phe-Ser-Pro-Gly-Trp-Gly-Thr-NH2
英文名称 Anti-Raptor
中文名称 mTOR相关调控蛋白抗体品牌
别 名 p150 target of rapamycin (TOR) scaffold protein containing WD repeats; P150 target of rapamycin (TOR)-scaffold protein; Regulatory Associated Protein of mTOR; KIAA1303; RPTOR_HUMAN; Regulatory-associated protein of mTOR; p150 target of rapamycin (TOR)-scaffold protein.
浓 度 1mg/1ml
规 格 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit
产品类型 一抗
研究领域 肿瘤 免疫学 信号转导 细胞凋亡 转录调节因子
蛋白分子量 predicted molecular weight: 147kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human Raptor
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 mTOR controls cell growth, in part by regulating p70 S6 kinase alpha (p70alpha) and eukaryotic initiation factor 4E binding protein 1 (4EBP1). Raptor is a 150 kDa mTOR binding protein that also binds 4EBP1 and p70alpha. The binding of Raptor to mTOR is necessary for the mTOR-catalyzed phosphorylation of 4EBP1 in vitro, and it strongly enhances the mTOR kinase activity toward p70alpha. Rapamycin or amino acid withdrawal increases, whereas insulin strongly inhibits, the recovery of 4EBP1 and raptor on 7-methyl-GTP Sepharose. Partial inhibition of raptor expression by RNA interference (RNAi) reduces mTOR-catalyzed 4EBP1 phosphorylation in vitro. RNAi of C. elegans raptor yields an array of phenotypes that closely resemble those produced by inactivation of Ce-TOR. Thus, raptor is an essential scaffold for the mTOR-catalyzed phosphorylation of 4EBP1 and mediates TOR action in vivo.
Function : Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation.
Subunit : Interacts with MTOR (By similarity). Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR and AKT1S1. mTORC1 binds to and is inhibited by FKBP12-rapamycin. Binds directly to 4EBP1 and RPS6KB1 independently of its association with MTOR. Binds preferentially to poorly or non-phosphorylated forms of EIF4EBP1, and this binding is critical to the ability of MTOR to catalyze phosphorylation. Forms a complex with MTOR under both leucine-rich and -poor conditions. Interacts with ULK1 in a nutrient-dependent manner; the interaction is reduced during starvation. Interacts (when phosphorylated by AMPK) with 14-3-3 protein, leading to inhibit its activity.
Subcellular Location : Cytoplasm. Lysosome. Note=Targeting to lysosomes depends on amino acid availability.
Tissue Specificity : Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta.
Post-translational modifications : In response to nutrient limitation, phosphorylated by AMPK; phosphorylation promotes interaction with 14-3-3 proteins, leading to negative regulation of the mTORC1 complex. In response to growth factors, phosphorylated at Ser-719, Ser-721 and Ser-722 by RPS6KA1; phosphorylation positively stimulates mTORC1 activity.
Similarity : Belongs to the WD repeat RAPTOR family.
Contains 7 WD repeats.
Database links : UniProtKB/Swiss-Prot:
mTOR相关调控蛋白抗体品牌具有高度的特异性，免疫组织化学正是利用了这一原理。先将组织或细胞中的某种化学物质提取出来，以此作为抗原或半抗原，通过免疫动物后获得特异性的抗体，再以此抗体去探测组织或细胞中的同类的抗原物质。由于抗原与抗体的复合物是无色的，因此还必须借助于组织化学的方法将抗原抗体结合的部位显示出来，以其达到对组织或细胞中的未知抗原进行定性，定位或定量的研究。
mTOR相关调控蛋白抗体品牌包括以下几个步骤：
1、制备抗原。
2、选择实验动物。
3、动物免疫。
4、试取血进行测试，看看是否成功免疫。
5、如果成功免疫，杀死实验动物，采集全部血清。
6、纯化出抗体。
7、鉴定抗体。包括纯度以及特异性。
mTOR相关调控蛋白抗体品牌的功能
抗体的主要功能是与抗原（包括外来的和自身的）相结合，从而有效地清除侵入机体内的微生物、寄生虫等异物，抗体（antibody）是一种应答抗原产生的、可与抗原特异性结合的蛋白质。每种抗体与特定的抗原决定基结合。这种结合可以使抗原失活，也可能无效但有时也会对机体造成病理性损害，如抗核抗体、抗双链DNA抗体、抗甲状腺球蛋白抗体等一些自身抗体的产生，对人体可造成危害。
mTOR相关调控蛋白抗体品牌的优点：
①耐热、耐酸、耐碱、抗有机溶剂以及金属离子，稳定性好，室温下可以长期保存；
②制备简单，操作方便，可以进行批量；
③不必免疫动物，且可获得免疫动物所不能得到的“抗体”；
④可以反复使用；
⑤价格低廉。因此，设计、合成既具有类似生物抗体的高亲合性和高专一性，又具有耐热、耐酸、耐碱且又可以长期稳定的人工抗体，在部分领域替代生物抗体以进行仿生分子识别，或者完成一些生物抗体所不能完成的工作，具有重要的科学意义，在化学、生命科学和环境科学等方面具有广阔的应用前景
公司专业供应各种进口、国产一抗及二抗，品牌有R&D、Santa Cruz、Bipec、Millipore等国际知名品牌，品种多达7000多种，由于产品的数量众多，产品的宿主、抗原来源及应用范围(WB、IH(F/P)、FC、ELISA、IP、IF等)有所不同。
phospho-HDAC1(Ser423+Ser421)磷酸化组蛋白去乙酰化酶1抗体规格:0.1ml
FRMD2/FRMPD1FRMD2蛋白抗体规格:0.2ml
TNFSF4/CD252瘤坏死因子配体超家族成员4抗体规格:0.1ml
LRRC59LRRC59蛋白抗体规格:0.2ml
MouseAnti-RabbitIgM/HRP辣根过氧化物酶标记的小鼠抗兔IgM规格:0.1ml
DCP异常凝酶原/脱-γ-羧基凝酶原抗体规格:0.2ml
CARD17/INCA凋亡加强结构域蛋白17抗体规格:0.2ml
PACAPR1苷酸环化酶激活肽受体1抗体规格:0.1ml
phospho-MST1R(Tyr1353)磷酸化原基因c-Met相关酪氨酸激酶抗体规格:0.1mlBNP-26 (porcine) Asp-Ser-Gly-Cys-Phe-Gly-Arg-Arg-Leu-Asp-Arg-Ile-Gly-Ser-Leu-Ser-Gly-Leu-Gly-Cys-Asn-Val-Leu-Arg-Arg-Tyr (Disulfide bridge:Cys4-Cys5)
Calcineurin Substrate Asp-Leu-Asp-Val-Pro-Ile-Pro-Gly-Arg-Phe-Asp-Arg-Arg-Val-Ser-Val-Ala-Ala-Glu
Cecropin B, Free Acid Lys-Trp-Lys-Val-Phe-Lys-Lys-Ile-Glu-Lys-Met-Gly-Arg-Asn-Ile-Arg-Asn-Gly-Ile-Val-Lys-Ala-Gly-Pro-Ala-Ile-Ala-Val-Leu-Gly-Glu-Ala-Lys-Ala-Leu
mTOR相关调控蛋白抗体品牌Crustacean Cardioactive Peptide, CCAP Pro-Phe-Cys-Asn-Ala-Phe-Thr-Gly-Cys(Disulfide bridge Cys3-Cys9)
Dynorphin A (1-8), porcine Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile
Exendin 4 (3-39) Glu-Gly-Thr-Phe-Thr-Ser-Asp-Leu-Ser-Lys-Gln-Met-Glu-Glu-Glu-Ala-Val-Arg-Leu-Phe-Ile-Glu-Trp-Leu-Lys-Asn-Gly-Gly-Pro-Ser-Ser-Gly-Ala-Pro-Pro-Pro-Ser-NH2
Gastrin I (1-14) (human) Pyr-Gly-Pro-Trp-Leu-Glu-Glu-Glu-Glu-Glu-Ala-Tyr-Gly-Trp
gp100 (619-627) Arg-Leu-Met-Lys-Gln-Asp-Phe-Ser-Val
Hexa His His-His-His-His-His-His
Hypertrehalosaemic Neuropeptide, Nauphoeta cinerea Pyr-Val-Asn-Phe-Ser-Pro-Gly-Trp-Gly-Thr-NH2
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文献和实验相关实验
包括多种细胞因子、趋化因子、蛋白酶和生长因子，它们可以通过促炎反应，促进衰老相关的病理特征。 SASP 受环境因素的影响，而在体内，一个主要的环境变量是氧含量，它在组织之间和组织内部是不同的。随着物种进化，哺乳动物细胞已能够通过不同的代谢途径迅速适应氧含量的变化。比如说，在低氧条件下，细胞通过激活 AMPK 信号通路和抑制 mTOR 的活性，从而使细胞的代谢模式由氧化磷酸化转变为糖酵解，并刺激血管生成。 前期也有研究表明，氧含量对衰老的发生有显著影响。低氧环境能够显著延缓培养细胞的各种类型的早衰
相关专题目的基因的表达调控生命活动丰富多彩、千变万化。但是万变不离其宗，不管如何变化都围绕着中心法则展开。核酸作为遗传物质指导蛋白质的表达，表达产生的一些特殊蛋白（如转录因子、调控蛋白）反过来又对DNA指导合成蛋白质的过程进行调控。对基因表达调控的研究一直是生物学研究热点，涉及到生命活动的各个过程，也是各类信号通路研究无法绕过的部分。当面对某个基因表达调控研究时，第一个想到的研究对象是什么？没错，就是基因的启动子。通过启动子区域对基因表达进行调控是最直接有效的手段，所以也是研究
【思路解读】一篇 10 分 SCI 文章，教你入手「铁死亡」的国自然课题设计思路
如何设计实验方案来验证呢？ 三、相关文献解读 接下来，给大家安利一篇 2020 年发表于《PNAS》的文章，题目为「Oncogenic activation of PI3K-AKT-mTOR signaling suppresses ferroptosis via SREBP-mediated lipogenesis」。 该文章首次揭示了 PI3K-AKT-mTOR 信号的高活性突变通过 SREBP1/SCD1 介导的脂肪生成保护癌细胞免受氧化应激和「铁死亡」的影响，为治疗携带 PI
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