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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 供应商:
上海雅吉生物科技有限公司
- 库存:
15
- 靶点:
Cytoplasm, Nucleus, Membrane
- 克隆性:
多克隆
- 抗原来源:
重组兔单抗
- 保质期:
一年
- 抗体英文名:
GAPDH
- 抗体名:
斑马鱼GAPDH抗体
- 标记物:
FITC
- 宿主:
重组兔单抗
- 适应物种:
斑马鱼
- 亚型:
IgG
- 形态:
Liquid
- 应用范围:
IP , IF , FC , ICC , IHC , WB
- 浓度:
1mg/ml
- 保存条件:
-20°
- 规格:
100ul
Recommended Dilutions:
WB: 1:1,000-1:5,000 ICC: 1:50-1:200 IHC: 1:50-1:200 FC: 1:10-1:100
.jpg)
Fig1: Western blot analysis of GAPDH on different cell lysates using anti-GAPDH antibody at 1/1,000 dilution.
Positive control:
Lane 1: NCCIT Lane 2: PC12
Lane 3: F9
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Fig2: ICC staining GAPDH in A549 cells (green). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
.jpg)
Fig3: ICC staining GAPDH in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
.jpg)
Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GAPDH antibody. Counter stained with hematoxylin.
.jpg)
Fig5: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-GAPDH antibody. Counter stained with hematoxylin.hematoxylin.
.jpg)
Fig6: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-GAPDH antibody. Counter stained with hematoxylin.
.jpg)
Fig7: Flow cytometric analysis of HepG2 cells with GAPDH antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).
Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Fig8: Western blot analysis of GAPDH on hybrid fish (crucian-carp) brain tissue lysate using anti-GAPDH antibody at斑马鱼GAPDH抗体
| 抗原介绍 | Description: GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. It participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. GAPDH is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. |
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