碱性磷酸酶-10556602103

Alkaline Phosphatase, EIA Grade from calf intestine, solution 10 556 602 103

Application
Marker enzyme for preparing antibody- / antigen-enzyme conjugates incorporated
in immunoassay reagents for colorimetric, fluorimetric and luminometric
detection.
Benefits
■ Synthesize stable and reproducible Alkaline Phosphatase (AP) conjugates
with AP, sourced from NZL intestines, that serves as a reliable
origin.
Properties
Nomenclature: Orthophosphoric-monoester phosphohydrolase (alkalineoptimum)
Molecular weight: ≥57 kD
Inhibitors: Pi, metal chelating agents, divalent heavy metal ions (e.g., Be2+,
Zn2+), many amino acids (e.g., L-phenylalanine, L-tryptophan, L-cysteine), iodosobenzoate,
iodoacet-amide.
Activators: Mg2+, Co 2+, Mn2+
pH optimum: 9.8
pH stability: 8.0
Thermal stability: Up to +40°C
Specification
Appearance: Clear, colourless solution in NaCl, 3 mol/l; MgCl2, 1 mmol/l;
ZnCl2, 0.1 mmol/l; Tea, 30 mmol/l
pH value: 7.0-8.0
Protein (A280, 1 mg/ml=1, against water): ≥10 mg/ml
Specific activity (+37°C, pNPP): ≥3000 U/mg
Alkaline Phosphatase (HPLC): ≥95 area% (HPLC profile added to certificate)
Amino groups: 8-16 mol/mol
Carbohydrates, n=2: No limit
pH 5.5 treatment (30 minutes): Corresponds to specification
Origin of bovine intestine: NZL
Stability: At +2 to +8°C within specification range for 15 months.