Cyclin D1周期素D1抗体

英文名称Cyclin D1
中文名称周期素D1抗体
别名：细胞周期素D1；CyclinD1；CyclinD1；B细胞CCl/淋巴瘤1；B细胞白血病1；B细胞白血病/淋巴瘤1；B细胞白血病1；B细胞淋巴瘤1蛋白；BCL-1；BCL1；BCL1癌基因；CCND 1；CCND1；CCND1蛋白；CCND1/FSTL3融合基因；CCND1/IGG1融合基因；CCND1/IGG1融合基因；DC1/IGLC1融合基因包括：CCND1/PTH融合基因；甲状旁腺腺瘤病1；PRAD1；FSTL3；CCND1人；AI317039；B细胞淋巴瘤1蛋白；Bcl 1；BCL -1；BCL癌基因；BC1癌基因；CCND1/FSTL3融合基因；包括CD1；Cyl 1；D11S28 7E；G1/S特异性细胞周期蛋白D1；G1/S-特异性CYLIM-D1。
Sample：
U937细胞（人）裂解物在30μg
原发性：抗细胞周期素B1（BS－057 2R）1/300稀释
次级：IrDyE800 CW山羊抗兔IgG 1/20000稀释
预测波段大小：48 kD
观察波段大小：50 kD
Sample：
U251细胞（人）裂解物在30μg
原发性：抗细胞周期素B1（BS－057 2R）1/300稀释
次级：IrDyE800 CW山羊抗兔IgG 1/20000稀释
预测波段大小：48 kD
观察波段大小：50 kD
Sample：
HeLa细胞（人）裂解物在30μg
原发性：抗细胞周期素B1（BS－057 2R）1/300稀释
次级：IrDyE800 CW山羊抗兔IgG 1/20000稀释
预测波段大小：48 kD
观察波段大小：50 kD
Sample：
K562细胞（人）裂解物30μg
原发性：抗细胞周期素B1（BS－057 2R）1/300稀释
次级：IrDyE800 CW山羊抗兔IgG 1/20000稀释
预测波段大小：48 kD
观察波段大小：50 kD
多聚甲醛固定，石蜡包埋（小鼠小肠）；用柠檬酸钠缓冲液（pH6.0）煮沸15min后提取抗原；用3%过氧化氢阻断内源性过氧化物酶20分钟；阻断缓冲液（正常山羊血清）37℃30min；用细胞周期蛋白B1进行抗体孵育多克隆抗体，未结合（BS-057 2R）1:400在4℃过夜，然后按照SP试剂盒（兔）（SP-023）指导和DAB染色进行操作。多聚甲醛固定，石蜡包埋（大鼠食管）；用柠檬酸钠缓冲液（pH6.0）煮沸15min后获得抗原；用3%过氧化氢阻断内源过氧化物酶20分钟；阻断缓冲液（正常山羊血清）37℃30min；用（cyclin B1）聚体抗体孵育。Lon抗体，未结合（BS-057 2R）1:400在4℃过夜，然后按照SP试剂盒（兔）（SP-023）指导和DAB染色进行操作。组织/细胞：小鼠胚胎组织；4%多聚甲醛固定和石蜡包埋；
抗原提取：柠檬酸缓冲液（0.01M，pH 6），15min煮沸，用3%过氧化氢阻断内源性过氧化物酶30min；37℃下阻断缓冲液（正常山羊血清，C-00 05）20 min；
孵育：抗Cyclin B1多克隆抗体，未结合（BS－057 2R）1:500，在4°C过夜，随后与二级抗体（SP-023）和DAB（C-00）染色结合。
组织/细胞：人结肠癌；4%多聚甲醛固定和石蜡包埋；
抗原提取：柠檬酸缓冲液（0.01M，pH 6），15min煮沸，用3%过氧化氢阻断内源性过氧化物酶30min；37℃下阻断缓冲液（正常山羊血清，C-00 05）20 min；
孵育：抗Cyclin B1多克隆抗体，未结合（BS－057 2R）1:200，在4°C过夜，随后与二级抗体（SP-023）和DAB（C-00）染色结合。
空白对照（蓝线）：A549（蓝色）。
原发性抗体（绿线）：兔抗细胞周期素B1抗体（BS－057 2R）。
稀释度：1μg/10 ^ 6细胞；
同种型对照抗体（橙色线）：兔IgG。
第二抗体（白蓝线）：F（ab′）2片段山羊抗兔IgG FITC。
稀释度：1μg/次。
协议
用2%聚甲醛（10分钟）固定细胞，室温下用0.1% pB-TWEN通透20分钟，室温下用一次抗体染色30分钟。然后在1×PBS／2% BSA／10%山羊血清中孵育细胞以阻断非特异性蛋白质-蛋白质相互作用，然后在室温下用抗体15分钟。第二抗体在室温下使用40分钟。进行20000个事件的采集。细胞：海拉
浓度：1:100
宿主/同种型：兔/IgG
流式细胞术分析HeLa（Green）上一级抗体（CATα：BS－057 2R）与同种型对照（在没有原代抗体（蓝色）的情况下），其次是Alexa For 488共轭山羊抗兔IgG（H+L）二级抗体。

细胞周期素D1蛋白（Cyclin D1）是细胞周期中的重要调控因子，它作用于细胞周期的G1→S期调控点，为G1期的限速步骤。
细胞周期蛋白D1-Cyclin D1的过度表达使细胞周期G1期缩短，细胞生长对有丝分裂原和粘附信号的需求降低，最终引发肿瘤的发生。该抗原的氨基酸序列抗原决定簇-结合位点，我们选在了细胞质的粗面内质网。越来越多的研究表明。CyclinD1在正常细胞周期及肿瘤的调节中起着重要作用。周期素D1/Bcl-1属于细胞周期调控蛋白家族成员之一，在细胞周期从G1进入S期中起到重要作用。周期素D1的过度表达与癌症的早发、肿瘤的进展和转移相关。该抗体可用于细胞周期方面的研究，

产品图片

Sample:  Mcf-7(Human) Cell Lysate at 30 ug Primary: Anti-Cyclin D1 (bs-0623R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kD Observed band size: 32 kD Sample: A549 Lysate at 40 ug Primary: Anti-CyclinD1 (bs-0623R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution Predicted band size: 32 kD Observed band size: 35 kD Sample:  A549 Cell (Human) Lysate at 30 ug Primary: Anti-Cyclin D1 (bs-0623R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 32 kD Observed band size: 35 kD Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cyclin D1) Polyclonal Antibody, Unconjugated (bs-0623R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat esophageal); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cyclin D1) Polyclonal Antibody, Unconjugated (bs-0623R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cyclin D1) Polyclonal Antibody, Unconjugated (bs-0623R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human cervical cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cyclin D1) Polyclonal Antibody, Unconjugated (bs-0623R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: human endometrium carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Cyclin D1 Polyclonal Antibody, Unconjugated(bs-0623R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Cyclin D1 Polyclonal Antibody, Unconjugated(bs-0623R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).