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北京博蕾德生物科技有限公司
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文献和实验Biological coating procedures for glass coverslips
because it is easily obtainable from rat tails. For short term cultures, collagen can be simply applied to glass coverslips and allowed to dry. 1. Dilute collagen solution 1:10 - 1:50 with 30% ethanol and spread over surface of sterile glass coverslip. 2. Air dry
Immunostaining cells on coverslips
Required solutions are given below. 1. Cells should be plated in 35 mm dishes containing coverslips. Fix cells when the cell density is not too high so the cells will not be crammed together. Usually plating at 1-2x105 cells per 35mm
Immunostaining of Cells Adherent to Coverslips
Procedure: 1) Immerse 18 mm2 glass coverslips in EtOH. In the tissue culture hood, individually pull out and carefully flame to sterilize. Allow to cool then place in 35 mm dishes, or in 6 well plates
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