- 询价
- invitrogen
- MA514905
- 2025年12月27日
- Immunoprecipitation, Western Blot
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Phospho-PDGFRA/PDGFRB (Tyr849, Tyr857) Monoclonal Antibody (T.564.8)
- 靶点：
Phospho-PDGFRA/PDGFRB (Tyr849, Tyr857)
- 应用范围：
Immunoprecipitation, Western Blot
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat
- 标记物：
Phosphorylated: Tyr849, Tyr857
- 克隆性：
Monoclonal
- 保存条件：
-20°C
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
Synthetic phosphopeptide corresponding to residues surrounding pTyr857 of human PDGF receptor ß
- 规格：
100 µL
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文献和实验Monoclonal Antibody Production
(note the removal of the HT)in a T25 flask (one for each positive clone)for later use (7) When the cells are ready for expansion out of the 24-well plate,resuspend them and pipet to the T25 flasks with feeder cells.Again leave a little bit in the 24
Monoclonal Antibody Production Protocol
(1)Cell Fusion and Selection Solutions: - IMDM - IMDM complete + 15% MCM. - PBS - PEG 50% ( w/v ): PEG 4000 ( 50% ) from Boehringer Manheim 1 243 268 - Aminopterin: Sigma, A 5159 (50X ) Prepare a T-150 flask with 170ml of IMDM
Monoclonal Antibody Production
Monoclonal Antibody Production Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform
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