- ￥3120
- biorbyt
- orb6660
- 英国
- 2025年12月04日
- ICC, IF, IHC-Fr, IHC-P
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Phospho-PDGFRA (Tyr849)/ PDGFRB (Tyr857) Rabbit Polyclonal Antibody抗体
- 抗体英文名：
Phospho-PDGFRA (Tyr849)/ PDGFRB (Tyr857) Rabbit Polyclonal Antibody
- 靶点：
PDGFRA
- 浓度：
1mg/ml
- 应用范围：
ICC, IF, IHC-Fr, IHC-P
- 宿主：
Rabbit
- 适应物种：
Human
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb6660
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated Synthesised phosphopeptide derived from human PDGF Receptor alpha around the phosphorylation site of Tyr849 SN(p-Y)VS
- 规格：
50 ul
别名:Receptor beta(phospho Y857); p-Receptor beta(phospho Y857); PDGF Receptor alpha(phospho Y849); p-PDGF Receptor alpha(phospho Y849); PDGF receptor alpha+beta(phospho Y849+Y857); PDGF receptor alpha+beta(phospho Tyr849+Tyr857); PDGF Receptor alpha(Tyr849)/PDGF Receptor beta(Tyr857); phospho-PDGFRA/PDGFRB(Tyr849/Tyr857); Platelet–dirived growth factor receptor-alpha; alpha platelet derived growth factor receptor; CD 140a; CD140a; CD140a antigen; MGC74795; PDGF alpha chain; PDGF R alpha; PDGFR 2; PDGFR A; PDGFR alpha; PDGFR2; PDGFRA; Platelet derived growth factor receptor 2; Platelet derived growth factor receptor alpha; Platelet derived growth factor receptor alpha polypeptide; PGFRA_HUMAN.
免疫原：KLH conjugated Synthesised phosphopeptide derived from human PDGF Receptor alpha around the phosphorylation site of Tyr849 SN(p-Y)VS
预测反应性:Bovine, Canine, Equine, Gallus, Mouse, Rat, Sheep
分子量:117 kDa
应用稀释比例：IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P16234
Note:For research use only.
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文献和实验运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 antibody (A) and Total-p38 antibody (B). Cell Based Elisa were performed directly in the 96-well plates using Phospho-p38 and Total-p38 antibodies (C). Figure 3: JNK detection with FACECells were serum-starved for 16 hours
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
。所以，我们建议使用微波炉或高压锅加热煮沸以达到最佳抗原修复效果。抗体：Phospho–Stat3 (Tyr705)(D3A7) XP. Rabbit mAb #9145样本：石蜡包埋人类肺部肿瘤样本煮沸设备：浴锅 (左)，微波炉 (中)，高压锅 (右)免疫染色—封闭在 IHC–P 中，我们建议在室温下在含有 Tween20 的 TBST 缓冲液和 5% 正常山羊血清 (NGS) 中封闭样品，时长 1 小时，以防止非特异性背景染色。在进行 IHC–F 时，在含有 0.3% Triton. X–100 的 1X
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