pUC18

pUC18

质粒名称: pUC18
其他名称:
质粒大小（bp）: 2686
抗性: Ampicillin
质粒类型: Unspecified
是否病毒: Unspecified
启动子:
稳转或瞬转: Unspecified
组成型或诱导性: Unspecified
表达水平:
蛋白标签:
测序引物:
GenBank号: L08752, A02710
其他属性: Expression Vector For Adjustable Expression Of Exogenous Genes In Prokaryotes; Patent number WO8809373-A/8 (01-DEC-1988). This patent sequence is not up-to-date. Expression vector with lacZ’ as an insertional detection marker. Medium is 1227 LB plus ampicillin. GenBank entry #L08752 is not current with commercial entries. Hosts: E.coli JM103, E.coli DH5alpha, E.coli DH10B, E.coli TB1, E.coli JM83, E.coli JM105, E.coli JM109, E.coli NM522, E.coli K-12, E.coli. Related vectors: pBR322, M13mp18, pUC12, pUC19.
质粒图谱:

 

质粒序列:

1	TCGCGCGTTT	CGGTGATGAC	GGTGAAAACC	TCTGACACAT	GCAGCTCCCG	50
51	GAGACGGTCA	CAGCTTGTCT	GTAAGCGGAT	GCCGGGAGCA	GACAAGCCCG	100
101	TCAGGGCGCG	TCAGCGGGTG	TTGGCGGGTG	TCGGGGCTGG	CTTAACTATG	150
151	CGGCATCAGA	GCAGATTGTA	CTGAGAGTGC	ACCATATGCG	GTGTGAAATA	200
201	CCGCACAGAT	GCGTAAGGAG	AAAATACCGC	ATCAGGCGCC	ATTCGCCATT	250
251	CAGGCTGCGC	AACTGTTGGG	AAGGGCGATC	GGTGCGGGCC	TCTTCGCTAT	300
301	TACGCCAGCT	GGCGAAAGGG	GGATGTGCTG	CAAGGCGATT	AAGTTGGGTA	350
351	ACGCCAGGGT	TTTCCCAGTC	ACGACGTTGT	AAAACGACGG	CCAGTGCCAA	400
401	GCTTGCATGC	CTGCAGGTCG	ACTCTAGAGG	ATCCCCGGGT	ACCGAGCTCG	450
451	AATTCGTAAT	CATGGTCATA	GCTGTTTCCT	GTGTGAAATT	GTTATCCGCT	500
501	CACAATTCCA	CACAACATAC	GAGCCGGAAG	CATAAAGTGT	AAAGCCTGGG	550
551	GTGCCTAATG	AGTGAGCTAA	CTCACATTAA	TTGCGTTGCG	CTCACTGCCC	600
601	GCTTTCCAGT	CGGGAAACCT	GTCGTGCCAG	CTGCATTAAT	GAATCGGCCA	650
651	ACGCGCGGGG	AGAGGCGGTT	TGCGTATTGG	GCGCTCTTCC	GCTTCCTCGC	700
701	TCACTGACTC	GCTGCGCTCG	GTCGTTCGGC	TGCGGCGAGC	GGTATCAGCT	750
751	CACTCAAAGG	CGGTAATACG	GTTATCCACA	GAATCAGGGG	ATAACGCAGG	800
801	AAAGAACATG	TGAGCAAAAG	GCCAGCAAAA	GGCCAGGAAC	CGTAAAAAGG	850
851	CCGCGTTGCT	GGCGTTTTTC	CATAGGCTCC	GCCCCCCTGA	CGAGCATCAC	900
901	AAAAATCGAC	GCTCAAGTCA	GAGGTGGCGA	AACCCGACAG	GACTATAAAG	950
951	ATACCAGGCG	TTTCCCCCTG	GAAGCTCCCT	CGTGCGCTCT	CCTGTTCCGA	1000
1001	CCCTGCCGCT	TACCGGATAC	CTGTCCGCCT	TTCTCCCTTC	GGGAAGCGTG	1050
1051	GCGCTTTCTC	AAAGCTCACG	CTGTAGGTAT	CTCAGTTCGG	TGTAGGTCGT	1100
1101	TCGCTCCAAG	CTGGGCTGTG	TGCACGAACC	CCCCGTTCAG	CCCGACCGCT	1150
1151	GCGCCTTATC	CGGTAACTAT	CGTCTTGAGT	CCAACCCGGT	AAGACACGAC	1200
1201	TTATCGCCAC	TGGCAGCAGC	CACTGGTAAC	AGGATTAGCA	GAGCGAGGTA	1250
1251	TGTAGGCGGT	GCTACAGAGT	TCTTGAAGTG	GTGGCCTAAC	TACGGCTACA	1300
1301	CTAGAAGAAC	AGTATTTGGT	ATCTGCGCTC	TGCTGAAGCC	AGTTACCTTC	1350
1351	GGAAAAAGAG	TTGGTAGCTC	TTGATCCGGC	AAACAAACCA	CCGCTGGTAG	1400
1401	CGGTGGTTTT	TTTGTTTGCA	AGCAGCAGAT	TACGCGCAGA	AAAAAAGGAT	1450
1451	CTCAAGAAGA	TCCTTTGATC	TTTTCTACGG	GGTCTGACGC	TCAGTGGAAC	1500
1501	GAAAACTCAC	GTTAAGGGAT	TTTGGTCATG	AGATTATCAA	AAAGGATCTT	1550
1551	CACCTAGATC	CTTTTAAATT	AAAAATGAAG	TTTTAAATCA	ATCTAAAGTA	1600
1601	TATATGAGTA	AACTTGGTCT	GACAGTTACC	AATGCTTAAT	CAGTGAGGCA	1650
1651	CCTATCTCAG	CGATCTGTCT	ATTTCGTTCA	TCCATAGTTG	CCTGACTCCC	1700
1701	CGTCGTGTAG	ATAACTACGA	TACGGGAGGG	CTTACCATCT	GGCCCCAGTG	1750
1751	CTGCAATGAT	ACCGCGAGAC	CCACGCTCAC	CGGCTCCAGA	TTTATCAGCA	1800
1801	ATAAACCAGC	CAGCCGGAAG	GGCCGAGCGC	AGAAGTGGTC	CTGCAACTTT	1850
1851	ATCCGCCTCC	ATCCAGTCTA	TTAATTGTTG	CCGGGAAGCT	AGAGTAAGTA	1900
1901	GTTCGCCAGT	TAATAGTTTG	CGCAACGTTG	TTGCCATTGC	TACAGGCATC	1950
1951	GTGGTGTCAC	GCTCGTCGTT	TGGTATGGCT	TCATTCAGCT	CCGGTTCCCA	2000
2001	ACGATCAAGG	CGAGTTACAT	GATCCCCCAT	GTTGTGCAAA	AAAGCGGTTA	2050
2051	GCTCCTTCGG	TCCTCCGATC	GTTGTCAGAA	GTAAGTTGGC	CGCAGTGTTA	2100
2101	TCACTCATGG	TTATGGCAGC	ACTGCATAAT	TCTCTTACTG	TCATGCCATC	2150
2151	CGTAAGATGC	TTTTCTGTGA	CTGGTGAGTA	CTCAACCAAG	TCATTCTGAG	2200
2201	AATAGTGTAT	GCGGCGACCG	AGTTGCTCTT	GCCCGGCGTC	AATACGGGAT	2250
2251	AATACCGCGC	CACATAGCAG	AACTTTAAAA	GTGCTCATCA	TTGGAAAACG	2300
2301	TTCTTCGGGG	CGAAAACTCT	CAAGGATCTT	ACCGCTGTTG	AGATCCAGTT	2350
2351	CGATGTAACC	CACTCGTGCA	CCCAACTGAT	CTTCAGCATC	TTTTACTTTC	2400
2401	ACCAGCGTTT	CTGGGTGAGC	AAAAACAGGA	AGGCAAAATG	CCGCAAAAAA	2450
2451	GGGAATAAGG	GCGACACGGA	AATGTTGAAT	ACTCATACTC	TTCCTTTTTC	2500
2501	AATATTATTG	AAGCATTTAT	CAGGGTTATT	GTCTCATGAG	CGGATACATA	2550
2551	TTTGAATGTA	TTTAGAAAAA	TAAACAAATA	GGGGTTCCGC	GCACATTTCC	2600
2601	CCGAAAAGTG	CCACCTGACG	TCTAAGAAAC	CATTATTATC	ATGACATTAA	2650
2651	CCTATAAAAA	TAGGCGTATC	ACGAGGCCCT	TTCGTC	2686