pUC18CMR

pUC18CMR

质粒名称: pUC18CMR
其他名称: 
质粒大小（bp）: 4276
抗性: Amp;Cam 
质粒类型: Unspecified
是否病毒: Unspecified 
启动子: 
稳转或瞬转: Unspecified 
组成型或诱导性: Unspecified 
表达水平: 
蛋白标签: 
测序引物: 
GenBank号: 
其他属性: Created by Moore, July 1995, under contract with NCBI. cmlR sequences (pBR325 cut with BclI/blunt ended with Klenow/cut with HincII) were ligated to a 2.6 kb HincII fragment of pUC4K. The 1.6 kb BamHI fragment was cloned into M13mp18;a 1.6 kb KpnI/HindIII fragment was cloned into the BamHI site of pUC18. pUC18CML (ATCC 37717) and pUC18CMR (ATCC 37718) differ only in the orientation of the cml cassette. In pUC18CMR, the lac promoter and the cml gene are in the same orientation. The cml cassette can be isolated by digestion with one enzyme (BamHI), by double digestion yielding blunt ends (HincII/SmaI), or by double digestion yielding overhangs for directional cloning. A plasmid containing the gene determining chloramphenicol resistance which is flanked by polylinker sites for ease in manipulation. Restriction digests of the clone give the following sizes (kb): EcoRI–3.4, 0.89; PstI–4.27; EcoRI/HindIII–2.45, 0.89, 0.75. (ATCC staff) Medium is 1235 LB plus tetracycline plus ampicillin. Hosts: E.coli XL-1, E.coli. Related vectors: pBR325, pUC18, pUC4K, pUC18CML. 
质粒图谱:

 

 

质粒序列:

1	tcgcgcgttt	cggtgatgac	ggtgaaaacc	tctgacacat	gcagctcccg	50
51	gagacggtca	cagcttgtct	gtaagcggat	gccgggagca	gacaagcccg	100
101	tcagggcgcg	tcagcgggtg	ttggcgggtg	tcggggctgg	cttaactatg	150
151	cggcatcaga	gcagattgta	ctgagagtgc	accatatgcg	gtgtgaaata	200
201	ccgcacagat	gcgtaaggag	aaaataccgc	atcaggcgcc	attcgccatt	250
251	caggctgcgc	aactgttggg	aagggcgatc	ggtgcgggcc	tcttcgctat	300
301	tacgccagct	ggcgaaaggg	ggatgtgctg	caaggcgatt	aagttgggta	350
351	acgccagggt	tttcccagtc	acgacgttgt	aaaacgacgg	ccagtgccaa	400
401	gcttgcatgc	ctgcaggtcg	actctagagg	atccgtcaac	acgggataat	450
451	accgcgccac	atagcagaac	tttaaaagtg	ctcatcattg	gaaaacgttc	500
501	ttcggggcga	aaactctcaa	ggatcttacc	gctgttgaga	tccagttcga	550
551	tgtaacccac	tcgtgcaccc	aactgatctt	cagcatcttt	tactttcacc	600
601	agcgtttctg	ggtgagcaaa	aacaggaagg	caaaatgccg	caaaaaaggg	650
651	aataagggcg	acacggaaat	gttgaatact	catactcttc	ctttttcaat	700
701	attattgaag	catttatcag	ggttattgtc	tcatgagcgg	atacatattt	750
751	gaatgtattt	agaaaaataa	acaaataggg	gttccgcgca	catttccccg	800
801	aaaagtgcca	cctgacgtct	aagaaaccat	tattatcatg	acattaacct	850
851	ataaaaatag	gcgtatcacg	aggccctttc	gtcttcgaat	aaatacctgt	900
901	gacggaagat	cacttcgcag	aataaataaa	tcctggtgtc	cctgttgata	950
951	ccgggaagcc	ctgggccaac	ttttggcgaa	aatgagacgt	tgatcggcac	1000
1001	gtaagaggtt	ccaactttca	ccataatgaa	ataagatcac	taccgggcgt	1050
1051	attttttgag	ttatcgagat	tttcaggagc	taaggaagct	aaaatggaga	1100
1101	aaaaaatcac	tggatatacc	accgttgata	tatcccaatg	gcatcgtaaa	1150
1151	gaacattttg	aggcatttca	gtcagttgct	caatgtacct	ataaccagac	1200
1201	cgttcagctg	gatattacgg	cctttttaaa	gaccgtaaag	aaaaataagc	1250
1251	acaagtttta	tccggccttt	attcacattc	ttgcccgcct	gatgaatgct	1300
1301	catccggaat	tccgtatggc	aatgaaagac	ggtgagctgg	tgatatggga	1350
1351	tagtgttcac	ccttgttaca	ccgttttcca	tgagcaaact	gaaacgtttt	1400
1401	catcgctctg	gagtgaatac	cacgacgatt	tccggcagtt	tctacacata	1450
1451	tattcgcaag	atgtggcgtg	ttacggtgaa	aacctggcct	atttccctaa	1500
1501	agggtttatt	gagaatatgt	ttttcgtctc	agccaatccc	tgggtgagtt	1550
1551	tcaccagttt	tgatttaaac	gtggccaata	tggacaactt	cttcgccccc	1600
1601	gttttcacca	tgggcaaata	ttatacgcaa	ggcgacaagg	tgctgatgcc	1650
1651	gctggcgatt	caggttcatc	atgccgtttg	tgatggcttc	catgtcggca	1700
1701	gaatgcttaa	tgaattacaa	cagtactgcg	atgagtggca	gggcggggcg	1750
1751	taattttttt	aaggcagtta	ttggtgccct	taaacgcctg	gtgctacgcc	1800
1801	tgaataagtg	ataataagcg	gatgaatggc	agaaattcga	aagcaaattc	1850
1851	gacccggtcg	tcggttcagg	gcagggtcgt	taaatagccg	cttatgtcta	1900
1901	ttgctggttt	accggtttat	tgactaccgg	aagcagtgtg	accgtgtgct	1950
1951	tctcaaatgc	ctgaggccag	tttgctcagg	ctctccccgt	ggaggtaata	2000
2001	attgacgata	tgatcgacgg	atccccgggt	accgagctcg	aattcgtaat	2050
2051	catggtcata	gctgtttcct	gtgtgaaatt	gttatccgct	cacaattcca	2100
2101	cacaacatac	gagccggaag	cataaagtgt	aaagcctggg	gtgcctaatg	2150
2151	agtgagctaa	ctcacattaa	ttgcgttgcg	ctcactgccc	gctttccagt	2200
2201	cgggaaacct	gtcgtgccag	ctgcattaat	gaatcggcca	acgcgcgggg	2250
2251	agaggcggtt	tgcgtattgg	gcgctcttcc	gcttcctcgc	tcactgactc	2300
2301	gctgcgctcg	gtcgttcggc	tgcggcgagc	ggtatcagct	cactcaaagg	2350
2351	cggtaatacg	gttatccaca	gaatcagggg	ataacgcagg	aaagaacatg	2400
2401	tgagcaaaag	gccagcaaaa	ggccaggaac	cgtaaaaagg	ccgcgttgct	2450
2451	ggcgtttttc	cataggctcc	gcccccctga	cgagcatcac	aaaaatcgac	2500
2501	gctcaagtca	gaggtggcga	aacccgacag	gactataaag	ataccaggcg	2550
2551	tttccccctg	gaagctccct	cgtgcgctct	cctgttccga	ccctgccgct	2600
2601	taccggatac	ctgtccgcct	ttctcccttc	gggaagcgtg	gcgctttctc	2650
2651	aaagctcacg	ctgtaggtat	ctcagttcgg	tgtaggtcgt	tcgctccaag	2700
2701	ctgggctgtg	tgcacgaacc	ccccgttcag	cccgaccgct	gcgccttatc	2750
2751	cggtaactat	cgtcttgagt	ccaacccggt	aagacacgac	ttatcgccac	2800
2801	tggcagcagc	cactggtaac	aggattagca	gagcgaggta	tgtaggcggt	2850
2851	gctacagagt	tcttgaagtg	gtggcctaac	tacggctaca	ctagaagaac	2900
2901	agtatttggt	atctgcgctc	tgctgaagcc	agttaccttc	ggaaaaagag	2950
2951	ttggtagctc	ttgatccggc	aaacaaacca	ccgctggtag	cggtggtttt	3000
3001	tttgtttgca	agcagcagat	tacgcgcaga	aaaaaaggat	ctcaagaaga	3050
3051	tcctttgatc	ttttctacgg	ggtctgacgc	tcagtggaac	gaaaactcac	3100
3101	gttaagggat	tttggtcatg	agattatcaa	aaaggatctt	cacctagatc	3150
3151	cttttaaatt	aaaaatgaag	ttttaaatca	atctaaagta	tatatgagta	3200
3201	aacttggtct	gacagttacc	aatgcttaat	cagtgaggca	cctatctcag	3250
3251	cgatctgtct	atttcgttca	tccatagttg	cctgactccc	cgtcgtgtag	3300
3301	ataactacga	tacgggaggg	cttaccatct	ggccccagtg	ctgcaatgat	3350
3351	accgcgagac	ccacgctcac	cggctccaga	tttatcagca	ataaaccagc	3400
3401	cagccggaag	ggccgagcgc	agaagtggtc	ctgcaacttt	atccgcctcc	3450
3451	atccagtcta	ttaattgttg	ccgggaagct	agagtaagta	gttcgccagt	3500
3501	taatagtttg	cgcaacgttg	ttgccattgc	tacaggcatc	gtggtgtcac	3550
3551	gctcgtcgtt	tggtatggct	tcattcagct	ccggttccca	acgatcaagg	3600
3601	cgagttacat	gatcccccat	gttgtgcaaa	aaagcggtta	gctccttcgg	3650
3651	tcctccgatc	gttgtcagaa	gtaagttggc	cgcagtgtta	tcactcatgg	3700
3701	ttatggcagc	actgcataat	tctcttactg	tcatgccatc	cgtaagatgc	3750
3751	ttttctgtga	ctggtgagta	ctcaaccaag	tcattctgag	aatagtgtat	3800
3801	gcggcgaccg	agttgctctt	gcccggcgtc	aatacgggat	aataccgcgc	3850
3851	cacatagcag	aactttaaaa	gtgctcatca	ttggaaaacg	ttcttcgggg	3900
3901	cgaaaactct	caaggatctt	accgctgttg	agatccagtt	cgatgtaacc	3950
3951	cactcgtgca	cccaactgat	cttcagcatc	ttttactttc	accagcgttt	4000
4001	ctgggtgagc	aaaaacagga	aggcaaaatg	ccgcaaaaaa	gggaataagg	4050
4051	gcgacacgga	aatgttgaat	actcatactc	ttcctttttc	aatattattg	4100
4101	aagcatttat	cagggttatt	gtctcatgag	cggatacata	tttgaatgta	4150
4151	tttagaaaaa	taaacaaata	ggggttccgc	gcacatttcc	ccgaaaagtg	4200
4201	ccacctgacg	tctaagaaac	cattattatc	atgacattaa	cctataaaaa	4250
4251	taggcgtatc	acgaggccct	ttcgtc	4276