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- 保存条件：
-2-8℃
- 保质期：
1年
- 英文名：
Mouse Cyclic AMP Direct Chemiluminescent CLIA Kit
- 库存：
大量
- 供应商：
上海钰博生物科技有限公司
- 规格：
盒
Mouse Cyclic AMP Direct Chemiluminescent CLIA Kit
Product Description
Mouse Cyclic AMP Direct Chemiluminescent CLIA Kit
Mouse Cyclic AMP (cAMP) Chemiluminescent Direct Immunoassay (CLIA) kits are designed to quantitatively measure cAMP present in cell lysates, plasma, urine, saliva, tissue and culture media samples. The supplied Sample Diluent will lyse cells, stabilize cAMP and stop phosphodiesterase activity. A cAMP standard is provided to generate a standard curve for the assay. The supplied Plate Primer solution is added to the wells of a coated white microtiter plate, followed by standards or diluted samples. A cAMP-peroxidase conjugate is then added to the wells. The binding reaction is initiated by the addition of a polyclonal antibody to cAMP. After a 2 hour incubation the plate is washed and chemiluminescent substrate is added. The substrate immediately reacts with the bound cAMP-peroxidase conjugate. The generated chemiluminescent glow signal is measured. The concentration of the cAMP in the sample is calculated, after making correction for the dilution.Cyclic AMP (cAMP) is one of the most important second messengers and a key intracellular regulator. Discovered by Sutherland and Rall in 1957, it functions as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH. Cyclic AMP is produced by the enzyme adenylate cyclase, and the enzyme is activated by the hormones glucagon and adrenaline and by G protein. cAMP decomposition into AMP is catalyzed by the enzyme phosphodiesterase. Other biological actions of cAMP include regulation of innate immune functioning, axon regeneration, cancer, and inflammation.
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文献和实验）即可直接从样品中提取高质量的Total RNA。 货号 品名（规格） 价格（RMB） 74104 RNeasy Mini Kit (50) 75142 RNeasy Midi Kit (10)* 75162 RNeasy Maxi Kit (12)* 74903 RNeasy Plant Mini Kit (20) * QIAGEN Oligotex Direct mRNA纯化系列 （Affymetrix推荐！） Direct系列产品无需酚氯仿抽提，不用乙醇沉淀或LiCl沉淀，也不用
（Maxi Kit）即可直接从样品中提取高质量的Total RNA。以下是部分产品信息，更大的包装信息或者详细操作手册欢迎致电基因有限公司或者点击产品名称查看。 货号 品名（规格） 价格（RMB） 74104 RNeasy Mini Kit (（50） 75142 RNeasy Midi Kit （10）* 75162 RNeasy Maxi Kit （12）* 74903 RNeasy Plant Mini Kit （20） * QIAGEN Oligotex Direct mRNA纯化系列
or overnight at 4°C with agitation. For a mouse monoclonal antibody, a goat anti-mouse HRP-conjugated antibody is recommended, for a rabbit polyclonal antibody, a goat anti-rabbit HRP-conjugated antibody is advisable. Wash the membrane five times
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