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再康生物
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50
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96 tests
Human Cyclic AMP Direct Chemiluminescent CLIA Kit
Catalog No.: IRAAKT2510
$395
Category: Assay and ELISA Kits
Product Description
Human Cyclic AMP Direct Chemiluminescent CLIA Kit
Human Cyclic AMP Direct Chemiluminescent CLIA Kits are designed to quantitatively measure cAMP present in cell lysates, plasma, urine, saliva, tissue and culture media samples. The supplied Sample Diluent will lyse cells, stabilize cAMP and stop phosphodiesterase activity. A cAMP standard is provided to generate a standard curve for the assay. The supplied Plate Primer solution is added to the wells of a coated white microtiter plate, followed by standards or diluted samples. A cAMP-peroxidase conjugate is then added to the wells.
The binding reaction is initiated by the addition of a polyclonal antibody to cAMP. After a 2 hour incubation the plate is washed and chemiluminescent substrate is added. The substrate immediately reacts with the bound cAMP-peroxidase conjugate. The generated chemiluminescent glow signal is measured. The concentration of the cAMP in the sample is calculated, after making correction for the dilution.
Cyclic AMP (cAMP) is one of the most important second messengers and a key intracellular regulator. Discovered by Sutherland and Rall in 1957, it functions as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH. Cyclic AMP is produced by the enzyme adenylate cyclase, and the enzyme is activated by the hormones glucagon and adrenaline and by G protein. cAMP decomposition into AMP is catalyzed by the enzyme phosphodiesterase. Other biological actions of cAMP include regulation of innate immune functioning, axon regeneration, cancer, and inflammation.
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文献和实验【进展|热点】Nature Immunology：骨髓基质细胞通过细胞间接触分泌SDF-1
2+) and transmit this across a network of BMSCs that are interconnected via Cx43-Cx45 gap junctions. The intercellular transmission of calcium sequentially activates signaling through cyclic AMP, protein kinase A and RalA that ultimately leads to higher expression
线粒体荧光探针大全：TMRM，Mitotracker，JC-1
valuable for analyzing human mitochondrial myopathies and related disorders.170,173,183,184 Alexa Fluor 488 and Alexa Fluor 594 conjugates of anti�COX subunit I are also available for direct staining of mitochondria (A21296, A21297; Figure 7.79). Mouse
肯定有偏向，但除非你对样品中的每种生物测序，否则你永远不知道偏向是什么。因此，你无法知道是否从每个细胞中提取出DNA。这仍是一个主要的障碍，我真的不知道如何去解决。” (文：Janelle Weaver博士/编译) 参考文献 1. Flores, G. E., J. B. Henley, and N. Fierer N. 2012. A direct PCR approach to accelerate analyses of human-associated
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