- ¥110 - 2270
- 百奥莱博
- 北京
- JN0012-MCW
- 2025年07月07日
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- 保存条件：
-20℃
- 保质期：
长期
- 英文名：
Ribonuclease H
- 库存：
802
- 供应商：
北京百奥莱博科技有限公司
特别声明：本产品及我公司所售其他产品均为科研类试剂产品，严禁用于药物、医疗及其他非科研用途。
北京百奥莱博专业生产销*(代"售")RNase H(国产,进口)，我公司供应的分子生物学试剂品类齐全，且具有优势价格，欢迎新老客户垂询订购。
名称：RNase H(国产,进口)
规格：200U
英文名：Ribonuclease H
品牌：百奥莱博
编号：JN0012
产地：国产|进口
RNase H是特异性分解RNA-DNA杂交体中的RNA链的核糖核酸内切酶，分子量为19,700，最适pH约为8.0。活性因子为Mg2+或Mn2+。活性单位为5U/ul。
产品组成：
| 组份 | JN0012-200U |
| RNase H (5U/ul) | 200ul |
| 10xRNase H Buffer | 1ml |
产品用途：
1、合成第二链cDNA时除去与第一链cDNA杂合的mRNA
2、DNA-RNA杂交体的鉴定
3、在Oligo(dT)存在下除去mRNA的Poly(A)末端
质量保证：经多次柱纯化，SDS-PAGE胶检测仅可见 清晰单一的目的条带；PCR方法检测无大肠杆菌DNA残留，无核酸内、外切酶污染。
活性定义：以poly(rA)•poly(dT)为底物，在30℃、pH7.7的条件下，20分钟内产生1 nmol酸可溶性物质所需要的酶量定义为1个活性单位（U）。
储存条件：-20℃
RNase H(国产,进口)正在火爆热销，除此之外，为您推荐下别热销产品：
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RNase H(国产,进口)关键词：RNase H,Ribonuclease H,JN0012
·重组人VAP-1(血管黏附蛋白1)(膜伯胺氧化酶)
编号：JN0768
英文名称：Recombinant Human Membrane Primary Amine Oxidase
规格：10μg|50μg|500μg|1mg
本品由我们的哺乳动物细胞表达系统制备而成，目的基因编码的Arg27-Asn763在C端含有His标签。
VAP-1/AOC3质量控制：＞95%(还原性SDS-PAGE)
VAP-1/AOC3制剂：液体
VAP-1/AOC3保存：收到货后请置于-20℃，可保存6个月，避免反复冻融。
关于VAP-1/AOC3：
Vascular adhesion protein-1(VAP-1) is a copper amine oxidase with a topaquinone cofactor.VAP-1 is a type II integral membrane protein, but a soluble form of the enzyme is present in human serum, and its level increases in diabetes and some inflammatory liver diseases. VAP-1 catalyzes the oxidative deamination of small primary amines such as methylamin*(代"e"), benzylamine, and aminoacetone in a reaction that produces an aldehyde, ammonia, and H2O2. VAP-1 vascular expression is regulated at sites of inflammation through its release from intracellular granules in which the protein is stored. The adhesive function of VAP-1 has been demonstrated in studies showing that the protein is important for the adherence of certain lymphocyte subtypes to inflamed endothelial tissues. VAP-1 mediated adhesion is involved in the process of leukocyte extravasation, an important feature of inflammatory responses. VAP-1 is considered to be a therapeutic target for diabetes, oxidative stress, and inflammatory diseases.
RNase H(国产,进口)关键词：RNase H,Ribonuclease H,JN0012
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我公司正在火爆促销工具酶系列产品，欢迎您的垂询选购RNase H(国产,进口)。
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- 内容
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文献和实验RNase H: Specificity, Mechanisms of Action, and Antiviral Target
The Ribonuclease (RNase) H is one of the four enzymes encoded by all retroviruses, including HIV. Its main activity is the hydrolysis of the RNA moiety in RNA–DNA hybrids. The RNase H ribonuclease is essential in the retroviral life cycle
Selective RNase H Cleavage of Target RNAs from a tRNA Scaffold
scaffold, using RNase H and two DNA oligonucleotides. After cleavage, we show that the RNA of interest can be isolated in a one-step purification. This method has, in particular, applications in structural investigations of RNA.
Question 1.What are the differences among RNase H, RNase A, RNase B and RNase C? 2.In your cDNA kits, RNase H is added in the second strand reaction to produce more nicked RNA as primers for DNA synthesis. In this repect
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