pRS313

pRS313

质粒名称: pRS313
其他名称: 
质粒大小（bp）: 4967
抗性: Ampicillin 
质粒类型: Yeast Expression
是否病毒: Unspecified 
启动子: 
稳转或瞬转: Unspecified 
组成型或诱导性: Unspecified 
表达水平: 
蛋白标签: 
测序引物: 
GenBank号: U03439 
其他属性: One of a series of pBluescript-based centromere vectors (ATCC 77142 to ATCC 77145, ATCC 77157 to ATCC 77158) differing in the yeast selectable marker gene. The order of the major features in this plasmid is: HIS3 – f1 ori (NaeI) – T7 promoter – lacZ'/MCS – T3 promoter – pMB1 ori – bla – CEN6 – ARSH4. A fragment (1.184 kb) containing the HIS3 gene was inserted into the NdeI site and a cassette containing CEN6 and the ARS associated with histone 4 (ARSH4) was inserted into the AatII site of pRSS56. All ends were blunted. pRSS56, constructed by ligating a PvuI fragment (bp 498-2412) of pBluescript KS+ to a PvuI fragment (bp 2850-730) of pBS(+), contains the KS MCS from pBluescript KS+ and the unique NdeI and AatII sites between bla and f1 origin of pBS(+). YC-type centromere vector permitting visual detection of recombinants and production of ssDNA in E.coli. Contains promoters for in vitro RNA synthesis, priming sites useful for sequencing, and encodes the lacZ alpha (lacZ') peptide. Restriction digests of the clone give the following sizes (kb): EcoRI–5.0; BamHI–5.0; PvuII–4.5, 0.48. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 416308 Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli, Saccharomyces cerevisiae YPH499, Saccharomyces cerevisiae YPH500, Saccharomyces cerevisiae YPH501. Related vectors: pRS314, pRS315, pRS316. 
质粒图谱:

 

 

质粒序列:

1	tcgcgcgttt	cggtgatgac	ggtgaaaacc	tctgacacat	gcagctcccg	50
51	gagacggtca	cagcttgtct	gtaagcggat	gccgggagca	gacaagcccg	100
101	tcagggcgcg	tcagcgggtg	ttggcgggtg	tcggggctgg	cttaactatg	150
151	cggcatcaga	gcagattgta	ctgagagtgc	accataattc	cgttttaaga	200
201	gcttggtgag	cgctaggagt	cactgccagg	tatcgtttga	acacggcatt	250
251	agtcagggaa	gtcataacac	agtcctttcc	cgcaattttc	tttttctatt	300
301	actcttggcc	tcctctagta	cactctatat	ttttttatgc	ctcggtaatg	350
351	attttcattt	ttttttttcc	acctagcgga	tgactctttt	tttttcttag	400
401	cgattggcat	tatcacataa	tgaattatac	attatataaa	gtaatgtgat	450
451	ttcttcgaag	aatatactaa	aaaatgagca	ggcaagataa	acgaaggcaa	500
501	agatgacaga	gcagaaagcc	ctagtaaagc	gtattacaaa	tgaaaccaag	550
551	attcagattg	cgatctcttt	aaagggtggt	cccctagcga	tagagcactc	600
601	gatcttccca	gaaaaagagg	cagaagcagt	agcagaacag	gccacacaat	650
651	cgcaagtgat	taacgtccac	acaggtatag	ggtttctgga	ccatatgata	700
701	catgctctgg	ccaagcattc	cggctggtcg	ctaatcgttg	agtgcattgg	750
751	tgacttacac	atagacgacc	atcacaccac	tgaagactgc	gggattgctc	800
801	tcggtcaagc	ttttaaagag	gccctactgg	cgcgtggagt	aaaaaggttt	850
851	ggatcaggat	ttgcgccttt	ggatgaggca	ctttccagag	cggtggtaga	900
901	tctttcgaac	aggccgtacg	cagttgtcga	acttggtttg	caaagggaga	950
951	aagtaggaga	tctctcttgc	gagatgatcc	cgcattttct	tgaaagcttt	1000
1001	gcagaggcta	gcagaattac	cctccacgtt	gattgtctgc	gaggcaagaa	1050
1051	tgatcatcac	cgtagtgaga	gtgcgttcaa	ggctcttgcg	gttgccataa	1100
1101	gagaagccac	ctcgcccaat	ggtaccaacg	atgttccctc	caccaaaggt	1150
1151	gttcttatgt	agtgacaccg	attatttaaa	gctgcagcat	acgatatata	1200
1201	tacatgtgta	tatatgtata	cctatgaatg	tcagtaagta	tgtatacgaa	1250
1251	cagtatgata	ctgaagatga	caaggtaatg	catcattcta	tacgtgtcat	1300
1301	tctgaacgag	gcgcgctttc	cttttttctt	tttgcttttt	cttttttttt	1350
1351	ctcttgaact	cgacggatca	tatgcggtgt	gaaataccgc	acagatgcgt	1400
1401	aaggagaaaa	taccgcatca	ggaaattgta	aacgttaata	ttttgttaaa	1450
1451	attcgcgtta	aatttttgtt	aaatcagctc	attttttaac	caataggccg	1500
1501	aaatcggcaa	aatcccttat	aaatcaaaag	aatagaccga	gatagggttg	1550
1551	agtgttgttc	cagtttggaa	caagagtcca	ctattaaaga	acgtggactc	1600
1601	caacgtcaaa	gggcgaaaaa	ccgtctatca	gggcgatggc	ccactacgtg	1650
1651	aaccatcacc	ctaatcaagt	tttttggggt	cgaggtgccg	taaagcacta	1700
1701	aatcggaacc	ctaaagggag	cccccgattt	agagcttgac	ggggaaagcc	1750
1751	ggcgaacgtg	gcgagaaagg	aagggaagaa	agcgaaagga	gcgggcgcta	1800
1801	gggcgctggc	aagtgtagcg	gtcacgctgc	gcgtaaccac	cacacccgcc	1850
1851	gcgcttaatg	cgccgctaca	gggcgcgtcg	cgccattcgc	cattcaggct	1900
1901	gcgcaactgt	tgggaagggc	gatcggtgcg	ggcctcttcg	ctattacgcc	1950
1951	agctggcgaa	ggggggatgt	gctgcaaggc	gattaagttg	ggtaacgcca	2000
2001	gggttttccc	agtcacgacg	ttgtaaaacg	acggccagtg	aattgtaata	2050
2051	cgactcacta	tagggcgaat	tggagctcca	ccgcggtggc	ggccgctcta	2100
2101	gaactagtgg	atcccccggg	ctgcaggaat	tcgatatcaa	gcttatcgat	2150
2151	accgtcgacc	tcgagggggg	gcccggtacc	cagcttttgt	tccctttagt	2200
2201	gagggttaat	tccgagcttg	gcgtaatcat	ggtcatagct	gtttcctgtg	2250
2251	tgaaattgtt	atccgctcac	aattccacac	aacataggag	ccggaagcat	2300
2301	aaagtgtaaa	gcctggggtg	cctaatgagt	gaggtaactc	acattaattg	2350
2351	cgttgcgctc	actgcccgct	ttccagtcgg	gaaacctgtc	gtgccagctg	2400
2401	cattaatgaa	tcggccaacg	cgcggggaga	ggcggtttgc	gtattgggcg	2450
2451	ctcttccgct	tcctcgctca	ctgactcgct	gcgctcggtc	gttcggctgc	2500
2501	ggcgagcggt	atcagctcac	tcaaaggcgg	taatacggtt	atccacagaa	2550
2551	tcaggggata	acgcaggaaa	gaacatgtga	gcaaaaggcc	agcaaaaggc	2600
2601	caggaaccgt	aaaaaggccg	cgttgctggc	gtttttccat	aggctcggcc	2650
2651	cccctgacga	gcatcacaaa	aatcgacgct	caagtcagag	gtggcgaaac	2700
2701	ccgacaggac	tataaagata	ccaggcgttc	ccccctggaa	gctccctcgt	2750
2751	gcgctctcct	gttccgaccc	tgccgcttac	cggatacctg	tccgcctttc	2800
2801	tcccttcggg	aagcgtggcg	ctttctcaat	gctcacgctg	taggtatctc	2850
2851	agttcggtgt	aggtcgttcg	ctccaagctg	ggctgtgtgc	acgaaccccc	2900
2901	cgttcagccc	gaccgctgcg	ccttatccgg	taactatcgt	cttgagtcca	2950
2951	acccggtaag	acacgactta	tcgccactgg	cagcagccac	tggtaacagg	3000
3001	attagcagag	cgaggtatgt	aggcggtgct	acagagttct	tgaagtggtg	3050
3051	gcctaactac	ggctacacta	gaaggacagt	atttggtatc	tgcgctctgc	3100
3101	tgaagccagt	taccttcgga	aaaagagttg	gtagctcttg	atccggcaaa	3150
3151	caaaccaccg	ctggtagcgg	tggttttttt	gtttgcaagc	agcagattac	3200
3201	gcgcagaaaa	aaaggatctc	aagaagatcc	tttgatcttt	tctacggggt	3250
3251	ctgacgctca	gtggaacgaa	aactcacgtt	aagggatttt	ggtcatgaga	3300
3301	ttatcaaaaa	ggatcttcac	ctagatcctt	ttaaattaaa	aatgaagttt	3350
3351	taaatcaatc	taaagtatat	atgagtaaac	ttggtctgac	agttaccaat	3400
3401	gcttaatcag	tgaggcacct	atctcagcga	tctgtctatt	tcgttcatcc	3450
3451	atagttgcct	gactgcccgt	cgtgtagata	actacgatac	gggagggctt	3500
3501	accatctggc	cccagtgctg	caatgatacc	gcgagaccca	cgctcaccgg	3550
3551	ctccagattt	atcagcaata	aaccagccag	ccggaagggc	cgagcgcaga	3600
3601	agtggtcctg	caactttatc	cgcctccatc	cagtctatta	attgttgccg	3650
3651	ggaagctaga	gtaagtagtt	cgccagttaa	tagtttgcgc	aacgttgttg	3700
3701	ccattgctac	aggcatcgtg	gtgtcacgct	cgtcgtttgg	tatggcttca	3750
3751	ttcagctccg	gttcccaacg	atcaaggcga	gttacatgat	cccccatgtt	3800
3801	gtgaaaaaaa	gcggttagct	ccttcggtcc	tccgatcgtt	gtcagaagta	3850
3851	agttggccgc	agtgttatca	ctcatggtta	tggcagcact	gcataattct	3900
3901	cttactgtca	tgccatccgt	aagatgcttt	tctgtgactg	gtgagtactc	3950
3951	aaccaagtca	ttctgagaat	agtgtatgcg	gcgaccgagt	tgctcttgcc	4000
4001	cggcgtcaat	acgggataat	accgcgccac	atagcagaac	tttaaaagtg	4050
4051	ctcatcattg	gaaaacgttc	ttcggggcga	aaactctcaa	ggatcttacc	4100
4101	gctgttgaga	tccagttcga	tgtaacccac	tcgtgcaccc	aactgatctt	4150
4151	cagcatcttt	tactttcacc	agcgtttctg	ggtgagcaaa	aacaggaagg	4200
4201	caaaatgccg	caaaaaaggg	aataagggcg	acacggaaat	gttgaatact	4250
4251	catactcttc	ctttttcaat	attattgaag	catttatcag	ggttattgtc	4300
4301	tcatgagcgg	atacatattt	gaatgtattt	agaaaaataa	acaaataggg	4350
4351	gttccgcgca	catttccccg	aaaagtgcca	cctgggtcct	tttcatcacg	4400
4401	tgctataaaa	ataattataa	tttaaatttt	ttaatataaa	tatataaatt	4450
4451	aaaaatagaa	agtaaaaaaa	gaaattaaag	aaaaaatagt	ttttgttttc	4500
4501	cgaagatgta	aaagactcta	gggggatcgc	caacaaatac	taccttttat	4550
4551	cttgctcttc	ctgctctcag	gtattaatgc	cgaattgttt	catcttgtct	4600
4601	gtgtagaaga	ccacacacga	aaatcctgtg	attttacatt	ttacttatcg	4650
4651	ttaatcgaat	gtatatctat	ttaatctgct	tttcttgtct	aataaatata	4700
4701	tatgtaaagt	acgctttttg	ttgaaatttt	ttaaaccttt	gtttattttt	4750
4751	ttttcttcat	tccgtaactc	ttctaccttc	tttatttact	ttctaaaatc	4800
4801	caaatacaaa	acataaaaat	aaataaacac	agagtaaatt	cccaaattat	4850
4851	tccatcatta	aaagatacga	ggcgcgtgta	agttacaggc	aagcgatccg	4900
4901	tcctaagaaa	ccattattat	catgacatta	acctataaaa	ataggcgtat	4950
4951	cacgaggccc	tttcgtc	4967