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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃保存
- 保质期:
有效期1年
- 英文名:
Progesterone receptor (PR) GFP Reporter Plasmid
- 库存:
大量
- 供应商:
翌圣生物科技(上海)股份有限公司
- 规格:
1μg
| 产品名称 | 产品编号 | 规格 | 储存 | 价格(元0 |
| Progesterone receptor (PR) GFP Reporter Plasmid (PR-GFP报告基因质粒) |
11710ES03 | 1µg | -20℃ | 3215.00 |
产品描述
PR-GFP报告基因是翌圣生物自主研发的用于检测PR转录活性水平为目的的报告基因。PGR(progesterone receptor)主要介导孕酮的生理作用。孕酮是动物内分泌系统、免疫系统、经血调控、组织修复与再生、炎症反应、血管发生、胚泡着床与维持妊娠交互作用的关键因子,尤其在动物生殖活动中起着非常重要的作用。
PR-GFP报告基因主要用于检测细胞Progesterone信号通路中的孕酮受体的转录活性、药物研究以及基因过表达和RNAi的表型分析等。
pGMPR-GFP是翌圣生物改造后的哺乳动物真核表达载体,在其多克隆位点插入了多个PR结合位点,可以高效地检测PR的激活水平。由于载体采用了GFP作为报告基因,更便于后续的检测。同时,对载体中预测出的其它转录因子以外的结合位点进行了适当的突变,增加了质粒的转录因子结合特异性。另外,由于质粒体积减小,使得PR-GFP报告基因更易于转染。
质粒图谱
质粒元件信息
| PR response element (PR) |
32-76 |
| Minimal TA promoter (pTA) |
105-127 |
| GFP reporter gene |
169-888 |
| SV40 late poly(A) signal |
923-1144 |
| SV40 early promoter |
1192-1609 |
| Synthetic neomycin phosphotransferase(Neor) coding region |
1635-2428 |
| Synthetic poly(A) signal |
2454-2501 |
| Synthetic Beta-lactamase(Ampr) coding region |
3617-4476 |
| Synthetic poly(A) signal/transcriptional pause site |
4582-4735 |
运输与保存方法
冰袋运输。-20℃保存。有效期1年。
使用说明
pGMPR-GFP可以采用常规转染方法转染哺乳动物细胞。
注意事项
1)本质粒未经翌圣生物允许不得用于任何商业用途,也不得移交给订货人实验室以外的任何人或单位。
2)为了您的健康,实验操作时请穿实验服和戴一次性手套。
3) 本产品仅作科研用途!
参考文献
[1] Toh MF, et al. Biological characterization of non-steroidal progestins from botanicals used for women's health.Steroids. 77(7):765-73(2012 Jun).
[2] Wu L, et al. 5'-AMP-activated protein kinase (AMPK) regulates progesterone receptor transcriptional activity in breast cancer cells.Biochem Biophys Res Commun. 416(1-2):172-7(2011).
[3] Han BY, et al. SUMO-2/3 can covalently bind to progesterone receptor B to regulate its transcriptional activity. Nan Fang Yi Ke Da Xue Xue Bao. 31(9):1493-7(2011).
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文献和实验[2] Wu L, et al. 5-AMP-activated protein kinase (AMPK) regulates progesterone receptor transcriptional activity in breast cancer cells.Biochem Biophys Res Commun. 416(1-2):172-7(2011).
[3] Han BY, et al. SUMO-2/3 can covalently bind to progesterone receptor B to regulate its transcriptional activity. Nan Fang Yi Ke Da Xue Xue Bao. 31(9):1493-7(2011).
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useful as a generic tag for studying protein synthesis, translocation, and other protein–protein interactions. GFP is also widely used as a reporter in many genetic techniques, including transposon mutagenesis, promoter/enhancer traps, and one-component
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of an organism and using it as the reporter. However, by far the commonest approach is to introduce a reporter gene into a cell to allow detection of a particular organism or to monitor its activity. The reporter gene can be located on a plasmid or to increase
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