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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
MR (Mineralocorticoid Receptor) GFP Reporter Plasmid
- 保质期:
有效期1年
- 供应商:
翌圣生物科技(上海)股份有限公司
- 保存条件:
-20度
- 规格:
1μg
产品信息
| 产品名称 |
产品编号 |
规格 |
价格(元) |
| MR (Mineralocorticoid Receptor) GFP Reporter Plasmid MR-GFP报告基因质粒 |
11770ES03 |
1 µg |
3365.00 |
产品描述
MR-GFP报告基因是翌圣生物自主研发的用于检测MR转录活性水平为目的的报告基因。MR (mineralocorticoid receptor)是核受体家族的一员,通过激素信号的传递和激活醛固醇靶基因的表达,调节多种生理病理反应。
MR-GFP报告基因主要应用于Mineralocorticoid Receptor信号通路、药物研究、相关基因的调控和功能的研究。
pGMMR-GFP是翌圣生物改造后的哺乳动物真核表达载体,在其多克隆位点插入了多个MR结合位点,可以高效地检测MR的激活水平。由于载体采用了GFP作为报告基因,更便于后续的检测。同时,对载体中预测出的其它转录因子以外的结合位点进行了适当的突变,增加了质粒的转录因子结合特异性。另外,由于质粒体积减小,使得MR-GFP报告基因更易于转染。
质粒图谱


质粒元件信息
| MR response element (MR) |
32-91 |
| Minimal TA promoter (pTA) |
120-142 |
| GFP reporter gene |
184-903 |
| SV40 late poly(A) signal |
938-1159 |
| SV40 early promoter |
1207-1624 |
| Synthetic neomycin phosphotransferase(Neor) coding region |
1650-2443 |
| Synthetic poly(A) signal |
2469-2516 |
| Synthetic Beta-lactamase(Ampr) coding region |
3632-4491 |
| Synthetic poly(A) signal/transcriptional pause site |
4597-4750 |
运输与保存方法
冰袋运输。-20℃保存。有效期1年。
使用说明
pGMMR-GFP可以采用常规转染方法转染哺乳动物细胞。
注意事项
1)本质粒未经翊圣生物允许不得用于任何商业用途,也不得移交给订货人实验室以外的任何人或单位。
2)为了您的健康,实验操作时请穿实验服和带一次性手套。
3)本产品仅作科研用途!
参考文献
[1] Lee HA, et al. Histone deacetylase inhibition attenuates transcriptional activity of mineralocorticoid receptor through its acetylation and prevents development of hypertension. Circ Res. 112(7):1004-12(2013).
[2] Kosaka H, et al. The L-, N-, and T-type triple calcium channel blocker benidipine acts as an antagonist of mineralocorticoid receptor, a member of nuclear receptor family. Eur J Pharmacol. 635(1-3):49-55(2010).
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文献和实验[2] Kosaka H, et al. The L-, N-, and T-type triple calcium channel blocker benidipine acts as an antagonist of mineralocorticoid receptor, a member of nuclear receptor family. Eur J Pharmacol. 635(1-3):49-55(2010).
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