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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃保存
- 保质期:
有效期1年
- 英文名:
VDR (Vitamin D Receptor) GFP Reporter Plasmid
- 库存:
大量
- 供应商:
翌圣生物科技(上海)股份有限公司
- 规格:
1μg
VDR报告基因是翊圣生物自主研发的用于检测VDR转录活性水平为目的的报告基因。VDR(Vitamin D Receptor)在本质上是类固醇受体超家族的成员之一。它在维持机体钙、磷代谢,调节细胞增殖、分化等方面起重要作用,已成为近年骨和内发泌学领域研究的焦点。
VDR报告基因主要应用于检测细胞VDRE信号通路中VDR的转录活性、药物研究以及基因过表达和RNAi的表型分析等。
pGMVDR-GFP是翊圣生物改造后的哺乳动物真核表达载体,在其多克隆位点插入了多个VDR结合位点,可以高效地检测VDR的激活水平。由于载体采用了GFP作为报告基因,更便于后续的检测。同时,对载体中预测出的其它转录因子以外的结合位点进行了适当的突变,增加了质粒的转录因子结合特异性。另外,由于质粒体积减小,使得VDR-GFP报告基因更易于转染。
质粒图谱
pGMVDR -GFP质粒测序引物
5’-TAGCAAAATAGGCTGTCCC-3’
运输与保存方法
冰袋运输。-20℃冻存。
使用说明
pGMVDR -GFP可以采用常规转染方法转染哺乳动物细胞。
注意事项
1)本质粒未经翊圣生物允许不得用于任何商业用途,也不得移交给订货人实验室以外的任何人或单位。
2)为了您的健康,实验操作时请穿实验服和带一次性手套。
参考文献
[1] Batie S, et al. Synthesis and biological evaluation of halogenated curcumin analogs as potential nuclear receptor selective agonists. Bioorg Med Chem. Dec 5(2012)..
[2] Choi M, et al.Vitamin D receptor activation induces peptide YY transcription in pancreatic islets. Endocrinology. 153(11):5188-99(2012)
[3] Gupta GK, et al. Agrawal DK.Decreased expression of vitamin D receptors in neointimal lesions following coronary artery angioplasty in atherosclerotic swine. PLoS One. 7(8):e42789(2012).
[4] Hidalgo AA, et al. Dexamethasone enhances 1alpha,25-dihydroxyvitamin D3 effects by increasing vitamin D receptor transcription. J Biol Chem. 286(42):36228-37(2011).
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