pMBP-P

pMBP-P

质粒名称: pMBP-P
其他名称:
质粒大小（bp）:6741
抗性:氨苄青霉素Amp
质粒类型:大肠杆菌载体；PET系列表达质粒
是否病毒:
启动子:
稳转或瞬转:
组成型或诱导性:
表达水平:
蛋白标签:N-MBP，N-Thrombin，C-6×His
测序引物:

5’测序引物:	M13：PUC 5 CGC CAG GGT TTT CCC AGT CAC GAC 3

GenBank号:
其他属性:
质粒图谱:

质粒序列:

LOCUS       Exported                6741 bp ds-DNA     circular SYN 05-AUG-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pMBP-P
SOURCE      synthetic DNA construct
ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6741)
AUTHORS   .
TITLE     Direct Submission
JOURNAL   Exported Tuesday, September 6, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES             Location/Qualifiers
source          1..6741
/organism=”synthetic DNA construct”
/mol_type=”other DNA”
promoter        3..80
/gene=”lacI (mutant)”
/note=”lacIq promoter”
/note=”In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold.”
CDS             81..1163
/codon_start=1
/gene=”lacI”
/product=”lac repressor”
/note=”lacI”
/note=”The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG).”
/translation=”MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ”
misc_feature    1406..1434
/note=”tac promoter”
/note=”strong E. coli promoter; hybrid between the trp and
lac UV5 promoters”
protein_bind    1442..1458
/bound_moiety=”lac repressor encoded by lacI”
/note=”lac operator”
/note=”The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG).”
CDS             1528..2703
/codon_start=1
/gene=”malE”
/product=”maltose binding protein from E. coli”
/note=”MBP”
/note=”This gene encodes a signal sequence that can direct
MBP to the bacterial periplasm.”
/translation=”MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNG
LAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAE
ITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDK
ELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLV
DLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPS
KPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKD
PRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT”
CDS             2803..2820
/codon_start=1
/product=”6xHis affinity tag”
/note=”6xHis”
/translation=”HHHHHH”
primer_bind     complement(2831..2847)
/note=”M13 fwd”
/note=”common sequencing primer, one of multiple similar
variants”
terminator      3196..3282
/gene=”Escherichia coli rrnB”
/note=”rrnB T1 terminator”
/note=”transcription terminator T1 from the E. coli rrnB
gene”
terminator      3374..3401
/note=”rrnB T2 terminator”
/note=”transcription terminator T2 from the E. coli rrnB
gene”
promoter        3421..3512
/gene=”bla”
/note=”AmpR promoter”
CDS             3513..4373
/codon_start=1
/gene=”bla”
/product=”beta-lactamase”
/note=”AmpR”
/note=”confers resistance to ampicillin, carbenicillin, and
related antibiotics”
/translation=”MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERSPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW”
rep_origin      complement(4415..4928)
/direction=LEFT
/note=”M13 ori”
/note=”M13 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis”
rep_origin      5039..5627
/direction=RIGHT
/note=”ori”
/note=”high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication”
misc_feature    5813..5955
/note=”bom”
/note=”basis of mobility region from pBR322″
CDS             complement(6057..6248)
/codon_start=1
/gene=”rop”
/product=”Rop protein, which maintains plasmids at low copy
number”
/note=”rop”
/translation=”MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL”
ORIGIN
1 ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga
61 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg
121 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa
181 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
241 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc
301 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
361 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
421 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
481 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
541 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc
601 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
661 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
721 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
781 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
841 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg
901 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
961 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
1021 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
1081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
1141 cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag
1201 gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg
1261 tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg
1321 tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt
1381 ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga
1441 attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga
1501 gcacttcacc aacaaggacc atagcatatg aaaataaaaa caggtgcacg catcctcgca
1561 ttatccgcat taacgacgat gatgttttcc gcctcggctc tcgccaaaat cgaagaaggt
1621 aaactggtaa tctggattaa cggcgataaa ggctataacg gtctcgctga agtcggtaag
1681 aaattcgaga aagataccgg aattaaagtc accgttgagc atccggataa actggaagag
1741 aaattcccac aggttgcggc aactggcgat ggccctgaca ttatcttctg ggcacacgac
1801 cgctttggtg gctacgctca atctggcctg ttggctgaaa tcaccccgga caaagcgttc
1861 caggacaagc tgtatccgtt tacctgggat gccgtacgtt acaacggcaa gctgattgct
1921 tacccgatcg ctgttgaagc gttatcgctg atttataaca aagatctgct gccgaacccg
1981 ccaaaaacct gggaagagat cccggcgctg gataaagaac tgaaagcgaa aggtaagagc
2041 gcgctgatgt tcaacctgca agaaccgtac ttcacctggc cgctgattgc tgctgacggg
2101 ggttatgcgt tcaagtatga aaacggcaag tacgacatta aagacgtggg cgtggataac
2161 gctggcgcga aagcgggtct gaccttcctg gttgacctga ttaaaaacaa acacatgaat
2221 gcagacaccg attactccat cgcagaagct gcctttaata aaggcgaaac agcgatgacc
2281 atcaacggcc cgtgggcatg gtccaacatc gacaccagca aagtgaatta tggtgtaacg
2341 gtactgccga ccttcaaggg tcaaccatcc aaaccgttcg ttggcgtgct gagcgcaggt
2401 attaacgccg ccagtccgaa caaagagctg gcaaaagagt tcctcgaaaa ctatctgctg
2461 actgatgaag gtctggaagc ggttaataaa gacaaaccgc tgggtgccgt agcgctgaag
2521 tcttacgagg aagagttggc gaaagatcca cgtattgccg ccaccatgga aaacgcccag
2581 aaaggtgaaa tcatgccgaa catcccgcag atgtccgctt tctggtatgc cgtgcgtact
2641 gcggtgatca acgccgccag cggtcgtcag actgtcgatg aagccctgaa agacgcgcag
2701 actaattcga gctctaacaa caacaacaac aacaacaata acaacctggg tatcgatact
2761 actgagaact atccgcgtgg atccgcagaa ttcagcgcta gccaccatca tcatcatcat
2821 taagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
2881 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
2941 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcag cttggctgtt
3001 ttggcggatg agataagatt ttcagcctga tacagattaa atcagaacgc agaagcggtc
3061 tgataaaaca gaatttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga
3121 actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag
3181 ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
3241 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
3301 aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg
3361 catcaaatta agcagaaggc catcctgacg gatggccttt ttgcgtttct acaaactctt
3421 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
3481 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
3541 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
3601 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
3661 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttctccaatg atgagcactt
3721 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
3781 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
3841 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
3901 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
3961 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
4021 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
4081 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
4141 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
4201 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
4261 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
4321 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
4381 accaagttta ctcatatata ctttagattg atttaccccg gttgataatc agaaaagccc
4441 caaaaacagg aagattgtat aagcaaatat ttaaattgta aacgttaata ttttgttaaa
4501 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
4561 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
4621 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
4681 gggcgatggc ccactacgtg aaccatcacc caaatcaagt tttttggggt cgaggtgccg
4741 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
4801 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
4861 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
4921 gggcgcgtaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
4981 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
5041 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
5101 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
5161 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
5221 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
5281 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
5341 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
5401 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
5461 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
5521 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
5581 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
5641 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
5701 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
5761 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
5821 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag
5881 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
5941 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
6001 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
6061 aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg
6121 tcgtgcagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc
6181 agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt
6241 ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg
6301 aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa
6361 cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag
6421 ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat
6481 cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt
6541 tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag
6601 cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa
6661 ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag
6721 gacccaacgc tgcccgaaat t
//