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- LMAI Bio
- 中国/上海
- LM3017
- 2025年12月13日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
pET28a-LIC(pET28aLIC)
- 库存：
大量
- 供应商：
联迈生物
- 规格：
1ug
质粒名称: pET28a-LIC
其他名称: None
质粒大小（bp）: 7328
抗性: Kanamycin
质粒类型: Bacterial Expression
是否病毒:
启动子:
稳转或瞬转:
组成型或诱导性:
表达水平: High Copy
蛋白标签: Hexahistidine tag with Thrombin cleavage
5’测序引物:T7
3’测序引物:T7-term
GenBank号:
其他属性:
质粒图谱:
质粒序列:
>Author sequence
tggcgaatgggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgacc
gctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccg
gctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcga
ccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccct
ttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatct
cggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgattta
acaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttcaggtggcacttttcggggaaat
gtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgaattaattct
tagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatattttt
gaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggta
tcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggtta
tcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagtttatgcatttctttcc
agacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcat
tcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaa
tgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaata
cctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatg
cttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattg
gcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattg
tcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatt
taatcgcggcctagagcaagacgtttcccgttgaatatggctcataacaccccttgtattactgtttatg
taagcagacagttttattgttcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagac
cccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaa
aaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaac
tggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaag
aactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgata
agtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggg
gggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagcta
tgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacag
gagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacct
ctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcg
gcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgatt
ctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcag
cgagtcagtgagcgaggaagcggaagagcgcctgatgcggtattttctccttacgcatctgtgcggtatt
tcacaccgcatatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatacact
ccgctatcgctacgtgactgggtcatggctgcgccccgacacccgccaacacccgctgacgcgccctgac
gggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagag
gttttcaccgtcatcaccgaaacgcgcgaggcagctgcggtaaagctcatcagcgtggtcgtgaagcgat
tcacagatgtctgcctgttcatccgcgtccagctcgttgagtttctccagaagcgttaatgtctggcttc
tgataaagcgggccatgttaagggcggttttttcctgtttggtcactgatgcctccgtgtaagggggatt
tctgttcatgggggtaatgataccgatgaaacgagagaggatgctcacgatacgggttactgatgatgaa
catgcccggttactggaacgttgtgagggtaaacaactggcggtatggatgcggcgggaccagagaaaaa
tcactcagggtcaatgccagcgcttcgttaatacagatgtaggtgttccacagggtagccagcagcatcc
tgcgatgcagatccggaacataatggtgcagggcgctgacttccgcgtttccagactttacgaaacacgg
aaaccgaagaccattcatgttgttgctcaggtcgcagacgttttgcagcagcagtcgcttcacgttcgct
cgcgtatcggtgattcattctgctaaccagtaaggcaaccccgccagcctagccgggtcctcaacgacag
gagcacgatcatgcgcacccgtggggccgccatgccggcgataatggcctgcttctcgccgaaacgtttg
gtggcgggaccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccga
tcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctac
gagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggag
ctgactgggttgaaggctctcaagggcatcggtcgagatcccggtgcctaatgagtgagctaacttacat
taattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcgg
ccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacggg
caacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgcccc
agcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgt
atcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgc
catctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttga
aaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatt
tatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctg
gtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttg
atgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatgg
catcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgc
cgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcg
cgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatca
gcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgc
ttccactttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataa
gagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactct
cttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtccgggatctcgacgct
ctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgc
aaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacg
ccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatatagg
cgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatcgagatctc
gatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcccctctagaaataa
ttttgtttaactttaagaaggagatataccatgggcagcagccatcatcatcatcatcacagcagcggcc
tggttccgcgtggtagtattatgagttctcctcctgaaagatccataacttcgtatagcatacattatac
gaagttatgcggccgcgacgtccacatatacctgccgttcactattatttagtgaaatgagatattatga
tattttctgaattgtgattaaaaaggcaactttatgcccatgcaacagaaactataaaaaatacagagaa
tgaaaagaaacagatagattttttagttctttaggcccgtagtctgcaaatccttttatgattttctatc
aaacaaaagaggaaaatagaccagttgcaatccaaacgagagtctaatagaatgaggtcgaaaagtaaat
cgcgcgggtttgttactgataaagcaggcaagacctaaaatgtgtaaagggcaaagtgtatactttggcg
tcaccccttacatattttaggtctttttttattgtgcgtaactaacttgccatcttcaaacaggagggct
ggaagaagcagaccgctaacacagtacataaaaaaggagacatgaacgatgaacatcaaaaagtttgcaa
aacaagcaacagtattaacctttactaccgcactgctggcaggaggcgcaactcaagcgtttgcgaaaga
aacgaaccaaaagccatataaggaaacatacggcatttcccatattacacgccatgatatgctgcaaatc
cctgaacagcaaaaaaatgaaaaatatcaagttcctgagttcgattcgtccacaattaaaaatatctctt
ctgcaaaaggcctggacgtttgggacagctggccattacaaaacgctgacggcactgtcgcaaactatca
cggctaccacatcgtctttgcattagccggagatcctaaaaatgcggatgacacatcgatttacatgttc
tatcaaaaagtcggcgaaacttctattgacagctggaaaaacgctggccgcgtctttaaagacagcgaca
aattcgatgcaaatgattctatcctaaaagaccaaacacaagaatggtcaggttcagccacatttacatc
tgacggaaaaatccgtttattctacactgatttctccggtaaacattacggcaaacaaacactgacaact
gcacaagttaacgtatcagcatcagacagctctttgaacatcaacggtgtagaggattataaatcaatct
ttgacggtgacggaaaaacgtatcaaaatgtacagcagttcatcgatgaaggcaactacagctcaggcga
caaccatacgctgagagatcctcactacgtagaagataaaggccacaaatacttagtatttgaagcaaac
actggaactgaagatggctaccaaggcgaagaatctttatttaacaaagcatactatggcaaaagcacat
cattcttccgtcaagaaagtcaaaaacttctgcaaagcgataaaaaacgcacggctgagttagcaaacgg
cgctctcggtatgattgagctaaacgatgattacacactgaaaaaagtgatgaaaccgctgattgcatct
aacacagtaacagatgaaattgaacgcgcgaacgtctttaaaatgaacggcaaatggtacctgttcactg
actcccgcggatcaaaaatgacgattgacggcattacgtctaacgatatttacatgcttggttatgtttc
taattctttaactggcccatacaagccgctgaacaaaactggccttgtgttaaaaatggatcttgatcct
aacgatgtaacctttacttactcacacttcgctgtacctcaagcgaaaggaaacaatgtcgtgattacaa
gctatatgacaaacagaggattctacgcagacaaacaatcaacgtttgcgcctagcttcctgctgaacat
caaaggcaagaaaacatctgttgtcaaagacagcatccttgaacaaggacaattaacagttaacaaataa
aaacgcaaaagaaaatgccgatatcctattggcattgacgtcaggtggcacttttcgaggagatcatgca
catgatgacgaagcttgcggccgcactcgagcaccaccaccaccaccactgagatccggctgctaacaaa
gcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctcta
aacgggtcttgaggggttttttgctgaaaggaggaactatatccggat
>26094 with T7
TTCCCTCTGAATATTTTGTTTAACTTTAAGAAGGAGATATACCATGGGCAGCAGCCATCATCATCATCAT
CACAGCAGCGGCCTGGTTCCGCGTGGTAGTATTATGAGTTCTCCTCCTGAAAGATCCATAACTTCGTATA
GCATACATTATACGAAGTTATGCGGCCGCGACGTCCACATATACCTGCCGTTCACTATTATTTAGTGAAA
TGAGATATTATGATATTTTCTGAATTGTGATTAAAAAGGCAACTTTATGCCCATGCAACAGAAACTATAA
AAAATACAGAGAATGAAAAGAAACAGATAGATTTTTTAGTTCTTTAGGCCCGTAGTCTGCAAATCCTTTT
ATGATTTTCTATCAAACAAAAGAGGAAAATAGACCAGTTGCAATCCAAACGAGAGTCTAATAGAATGAGG
TCGAAAAGTAAATCGCGCGGGTTTGTTACTGATAAAGCAGGCAAGACCTAAAATGTGTAAAGGGCAAAGT
GTATACTTTGGCGTCACCCCTTACATATTTTAGGTCTTTTTTTATTGTGCGTAACTAACTTGCCATCTTC
AAACAGGAGGGCTGGAAGAAGCAGACCGCTAACACAGTACATAAAAAAGGAGACATGAACGATGAACATC
AAAAAGTTTGCAAAACAAGCAACAGTATTAACCTTTACTACCGCACTGCTGGCAGGAGGCGCAACTCAAG
CGTTTGCGAAAGAAACGAACCAAAAGCCATATAAGGAAACATACGGCATTTCCCATATTACACGCCATGA
TATGCTGCAAATCCCTGAACAGCAAAAAAATGAAAAATATAAAGTTCCTGAGTTCGATTCGTCCACAATT
AAAAATA
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- 作者
- 内容
- 询问日期
文献和实验The protocol for LIC by Exonuclease III
The protocol for LIC by Exonuclease III 梁耀极 1. Design the primers with 15-bp overlap; 2. Digest the vector by proper restriction enzyme; For getting high quality vectors, there are some good advices as follows: 1) The restriction
查、基因筛选及图位克隆有益基因的最好材料，也是永久保存基因资源的最好方法之一。 至于作用就太多了，你自己google一下，发现的是你好多做不了，而不是不知道怎么做？ huangyuan62 楼主,请问能交换你的BAC质粒吗？pBACe3.6,pBeloBAC11等都可以。 本人有以下质粒和菌种,可全部用于交换 pET28a,pET32a,pETDsb,pETGST,pETTrx，pTrc—CKS, pGEX4T
dmdfe 本人将GFP基因后面加了一段大约110bp的序列，插入PET28A的BamH1和Hind3位点，最后在BL21中表达。 经SDS-PAGE验证表明： 目的蛋白 大量存在菌体裂解后沉淀即包涵体中，但是该沉淀无色。 少量存在于上清中，上清显微弱的绿色。 看文献大量报道GFP融合蛋白几乎都形成可溶性表达，目的蛋白在上清中，并且诱导条件没有太大要求。 想请教一下各位做过GFP原核表达的经验
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