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- 详细信息
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- 技术资料
- 保存条件:
-20℃
- 保质期:
6个月
- 英文名:
pET-21b(+)(pET21b)(60908-2757)
- 库存:
大量
- 供应商:
联迈生物
- 规格:
1ug
质粒名称: pET-21 b (+)
其他名称:
质粒大小(bp): 5442
抗性: Ampicillin (氨苄青霉素)
质粒类型: 大肠杆菌表达载体
是否病毒: Nonviral
启动子:
稳转或瞬转: Transient
组成型或诱导性: Constitutive
表达水平: High
蛋白标签: N-T7, C-His
5’测序引物:T7
5’测序引物序列:5′-TAATACGACTCACTATAGGG-3′
GenBank号:
其他属性: Same as pET24abcd(+) but ampR; a,b,c,d vary by MCS; The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.
质粒图谱:
| Feature Name | Start | End | |
|---|---|---|---|
| T7_terminator | 129 | 1 | |
| T7_Terminal_primer | 69 | 87 | |
| T7_leader | 238 | 206 | |
| Xpress_fwd_primer | 239 | 221 | |
| T7_transl_en_RBS | 263 | 247 | |
| lacO | 308 | 281 | |
| T7_promoter | 326 | 308 | |
| tet (300 – 563) | 358 | 621 | |
| pBRrevBam_primer | 429 | 410 | |
| lacI | 704 | 1795 | |
| tet (576 – 851) | 1854 | 2129 | |
| ROP | 2604 | 2795 | |
| pGEX_3_primer | 2811 | 2789 | |
| pBR322_origin | 3829 | 3210 | |
| Ampicillin | 4844 | 3984 | |
| AmpR_promoter | 4914 | 4886 |
| ORF | Start | End | |
|---|---|---|---|
| ORF frame 1 | 463 | 963 | |
| ORF frame 2 | 836 | 1795 | |
| ORF frame 2 | 4844 | 3984 |
| Enzyme Name | Cut |
|---|---|
| XhoI | 158 |
| NotI | 166 |
| EagI | 166 |
| HindIII | 173 |
| SalI | 179 |
| SacI | 190 |
| EcoRI | 192 |
| BamHI | 198 |
| NheI | 230 |
| NdeI | 237 |
| XbaI | 275 |
| BglII | 341 |
| BclI | 1077 |
| ApaI | 1274 |
| EcoRV | 1513 |
| HpaI | 1569 |
| PstI | 4302 |
质粒序列:
| 1 | atccggatat | agttcctcct | ttcagcaaaa | aacccctcaa | gacccgttta | 50 |
| 51 | gaggccccaa | ggggttatgc | tagttattgc | tcagcggtgg | cagcagccaa | 100 |
| 101 | ctcagcttcc | tttcgggctt | tgttagcagc | cggatctcag | tggtggtggt | 150 |
| 151 | ggtggtgctc | gagtgcggcc | gcaagcttgt | cgacggagct | cgaattcgga | 200 |
| 201 | tcccgaccca | tttgctgtcc | accagtcatg | ctagccatat | gtatatctcc | 250 |
| 251 | ttcttaaagt | taaacaaaat | tatttctaga | ggggaattgt | tatccgctca | 300 |
| 301 | caattcccct | atagtgagtc | gtattaattt | cgcgggatcg | agatctcgat | 350 |
| 351 | cctctacgcc | ggacgcatcg | tggccggcat | caccggcgcc | acaggtgcgg | 400 |
| 401 | ttgctggcgc | ctatatcgcc | gacatcaccg | atggggaaga | tcgggctcgc | 450 |
| 451 | cacttcgggc | tcatgagcgc | ttgtttcggc | gtgggtatgg | tggcaggccc | 500 |
| 501 | cgtggccggg | ggactgttgg | gcgccatctc | cttgcatgca | ccattccttg | 550 |
| 551 | cggcggcggt | gctcaacggc | ctcaacctac | tactgggctg | cttcctaatg | 600 |
| 601 | caggagtcgc | ataagggaga | gcgtcgagat | cccggacacc | atcgaatggc | 650 |
| 651 | gcaaaacctt | tcgcggtatg | gcatgatagc | gcccggaaga | gagtcaattc | 700 |
| 701 | agggtggtga | atgtgaaacc | agtaacgtta | tacgatgtcg | cagagtatgc | 750 |
| 751 | cggtgtctct | tatcagaccg | tttcccgcgt | ggtgaaccag | gccagccacg | 800 |
| 801 | tttctgcgaa | aacgcgggaa | aaagtggaag | cggcgatggc | ggagctgaat | 850 |
| 851 | tacattccca | accgcgtggc | acaacaactg | gcgggcaaac | agtcgttgct | 900 |
| 901 | gattggcgtt | gccacctcca | gtctggccct | gcacgcgccg | tcgcaaattg | 950 |
| 951 | tcgcggcgat | taaatctcgc | gccgatcaac | tgggtgccag | cgtggtggtg | 1000 |
| 1001 | tcgatggtag | aacgaagcgg | cgtcgaagcc | tgtaaagcgg | cggtgcacaa | 1050 |
| 1051 | tcttctcgcg | caacgcgtca | gtgggctgat | cattaactat | ccgctggatg | 1100 |
| 1101 | accaggatgc | cattgctgtg | gaagctgcct | gcactaatgt | tccggcgtta | 1150 |
| 1151 | tttcttgatg | tctctgacca | gacacccatc | aacagtatta | ttttctccca | 1200 |
| 1201 | tgaagacggt | acgcgactgg | gcgtggagca | tctggtcgca | ttgggtcacc | 1250 |
| 1251 | agcaaatcgc | gctgttagcg | ggcccattaa | gttctgtctc | ggcgcgtctg | 1300 |
| 1301 | cgtctggctg | gctggcataa | atatctcact | cgcaatcaaa | ttcagccgat | 1350 |
| 1351 | agcggaacgg | gaaggcgact | ggagtgccat | gtccggtttt | caacaaacca | 1400 |
| 1401 | tgcaaatgct | gaatgagggc | atcgttccca | ctgcgatgct | ggttgccaac | 1450 |
| 1451 | gatcagatgg | cgctgggcgc | aatgcgcgcc | attaccgagt | ccgggctgcg | 1500 |
| 1501 | cgttggtgcg | gatatctcgg | tagtgggata | cgacgatacc | gaagacagct | 1550 |
| 1551 | catgttatat | cccgccgtta | accaccatca | aacaggattt | tcgcctgctg | 1600 |
| 1601 | gggcaaacca | gcgtggaccg | cttgctgcaa | ctctctcagg | gccaggcggt | 1650 |
| 1651 | gaagggcaat | cagctgttgc | ccgtctcact | ggtgaaaaga | aaaaccaccc | 1700 |
| 1701 | tggcgcccaa | tacgcaaacc | gcctctcccc | gcgcgttggc | cgattcatta | 1750 |
| 1751 | atgcagctgg | cacgacaggt | ttcccgactg | gaaagcgggc | agtgagcgca | 1800 |
| 1801 | acgcaattaa | tgtaagttag | ctcactcatt | aggcaccggg | atctcgaccg | 1850 |
| 1851 | atgcccttga | gagccttcaa | cccagtcagc | tccttccggt | gggcgcgggg | 1900 |
| 1901 | catgactatc | gtcgccgcac | ttatgactgt | cttctttatc | atgcaactcg | 1950 |
| 1951 | taggacaggt | gccggcagcg | ctctgggtca | ttttcggcga | ggaccgcttt | 2000 |
| 2001 | cgctggagcg | cgacgatgat | cggcctgtcg | cttgcggtat | tcggaatctt | 2050 |
| 2051 | gcacgccctc | gctcaagcct | tcgtcactgg | tcccgccacc | aaacgtttcg | 2100 |
| 2101 | gcgagaagca | ggccattatc | gccggcatgg | cggccccacg | ggtgcgcatg | 2150 |
| 2151 | atcgtgctcc | tgtcgttgag | gacccggcta | ggctggcggg | gttgccttac | 2200 |
| 2201 | tggttagcag | aatgaatcac | cgatacgcga | gcgaacgtga | agcgactgct | 2250 |
| 2251 | gctgcaaaac | gtctgcgacc | tgagcaacaa | catgaatggt | cttcggtttc | 2300 |
| 2301 | cgtgtttcgt | aaagtctgga | aacgcggaag | tcagcgccct | gcaccattat | 2350 |
| 2351 | gttccggatc | tgcatcgcag | gatgctgctg | gctaccctgt | ggaacaccta | 2400 |
| 2401 | catctgtatt | aacgaagcgc | tggcattgac | cctgagtgat | ttttctctgg | 2450 |
| 2451 | tcccgccgca | tccataccgc | cagttgttta | ccctcacaac | gttccagtaa | 2500 |
| 2501 | ccgggcatgt | tcatcatcag | taacccgtat | cgtgagcatc | ctctctcgtt | 2550 |
| 2551 | tcatcggtat | cattaccccc | atgaacagaa | atccccctta | cacggaggca | 2600 |
| 2601 | tcagtgacca | aacaggaaaa | aaccgccctt | aacatggccc | gctttatcag | 2650 |
| 2651 | aagccagaca | ttaacgcttc | tggagaaact | caacgagctg | gacgcggatg | 2700 |
| 2701 | aacaggcaga | catctgtgaa | tcgcttcacg | accacgctga | tgagctttac | 2750 |
| 2751 | cgcagctgcc | tcgcgcgttt | cggtgatgac | ggtgaaaacc | tctgacacat | 2800 |
| 2801 | gcagctcccg | gagacggtca | cagcttgtct | gtaagcggat | gccgggagca | 2850 |
| 2851 | gacaagcccg | tcagggcgcg | tcagcgggtg | ttggcgggtg | tcggggcgca | 2900 |
| 2901 | gccatgaccc | agtcacgtag | cgatagcgga | gtgtatactg | gcttaactat | 2950 |
| 2951 | gcggcatcag | agcagattgt | actgagagtg | caccatatat | gcggtgtgaa | 3000 |
| 3001 | ataccgcaca | gatgcgtaag | gagaaaatac | cgcatcaggc | gctcttccgc | 3050 |
| 3051 | ttcctcgctc | actgactcgc | tgcgctcggt | cgttcggctg | cggcgagcgg | 3100 |
| 3101 | tatcagctca | ctcaaaggcg | gtaatacggt | tatccacaga | atcaggggat | 3150 |
| 3151 | aacgcaggaa | agaacatgtg | agcaaaaggc | cagcaaaagg | ccaggaaccg | 3200 |
| 3201 | taaaaaggcc | gcgttgctgg | cgtttttcca | taggctccgc | ccccctgacg | 3250 |
| 3251 | agcatcacaa | aaatcgacgc | tcaagtcaga | ggtggcgaaa | cccgacagga | 3300 |
| 3301 | ctataaagat | accaggcgtt | tccccctgga | agctccctcg | tgcgctctcc | 3350 |
| 3351 | tgttccgacc | ctgccgctta | ccggatacct | gtccgccttt | ctcccttcgg | 3400 |
| 3401 | gaagcgtggc | gctttctcat | agctcacgct | gtaggtatct | cagttcggtg | 3450 |
| 3451 | taggtcgttc | gctccaagct | gggctgtgtg | cacgaacccc | ccgttcagcc | 3500 |
| 3501 | cgaccgctgc | gccttatccg | gtaactatcg | tcttgagtcc | aacccggtaa | 3550 |
| 3551 | gacacgactt | atcgccactg | gcagcagcca | ctggtaacag | gattagcaga | 3600 |
| 3601 | gcgaggtatg | taggcggtgc | tacagagttc | ttgaagtggt | ggcctaacta | 3650 |
| 3651 | cggctacact | agaaggacag | tatttggtat | ctgcgctctg | ctgaagccag | 3700 |
| 3701 | ttaccttcgg | aaaaagagtt | ggtagctctt | gatccggcaa | acaaaccacc | 3750 |
| 3751 | gctggtagcg | gtggtttttt | tgtttgcaag | cagcagatta | cgcgcagaaa | 3800 |
| 3801 | aaaaggatct | caagaagatc | ctttgatctt | ttctacgggg | tctgacgctc | 3850 |
| 3851 | agtggaacga | aaactcacgt | taagggattt | tggtcatgag | attatcaaaa | 3900 |
| 3901 | aggatcttca | cctagatcct | tttaaattaa | aaatgaagtt | ttaaatcaat | 3950 |
| 3951 | ctaaagtata | tatgagtaaa | cttggtctga | cagttaccaa | tgcttaatca | 4000 |
| 4001 | gtgaggcacc | tatctcagcg | atctgtctat | ttcgttcatc | catagttgcc | 4050 |
| 4051 | tgactccccg | tcgtgtagat | aactacgata | cgggagggct | taccatctgg | 4100 |
| 4101 | ccccagtgct | gcaatgatac | cgcgagaccc | acgctcaccg | gctccagatt | 4150 |
| 4151 | tatcagcaat | aaaccagcca | gccggaaggg | ccgagcgcag | aagtggtcct | 4200 |
| 4201 | gcaactttat | ccgcctccat | ccagtctatt | aattgttgcc | gggaagctag | 4250 |
| 4251 | agtaagtagt | tcgccagtta | atagtttgcg | caacgttgtt | gccattgctg | 4300 |
| 4301 | caggcatcgt | ggtgtcacgc | tcgtcgtttg | gtatggcttc | attcagctcc | 4350 |
| 4351 | ggttcccaac | gatcaaggcg | agttacatga | tcccccatgt | tgtgcaaaaa | 4400 |
| 4401 | agcggttagc | tccttcggtc | ctccgatcgt | tgtcagaagt | aagttggccg | 4450 |
| 4451 | cagtgttatc | actcatggtt | atggcagcac | tgcataattc | tcttactgtc | 4500 |
| 4501 | atgccatccg | taagatgctt | ttctgtgact | ggtgagtact | caaccaagtc | 4550 |
| 4551 | attctgagaa | tagtgtatgc | ggcgaccgag | ttgctcttgc | ccggcgtcaa | 4600 |
| 4601 | tacgggataa | taccgcgcca | catagcagaa | ctttaaaagt | gctcatcatt | 4650 |
| 4651 | ggaaaacgtt | cttcggggcg | aaaactctca | aggatcttac | cgctgttgag | 4700 |
| 4701 | atccagttcg | atgtaaccca | ctcgtgcacc | caactgatct | tcagcatctt | 4750 |
| 4751 | ttactttcac | cagcgtttct | gggtgagcaa | aaacaggaag | gcaaaatgcc | 4800 |
| 4801 | gcaaaaaagg | gaataagggc | gacacggaaa | tgttgaatac | tcatactctt | 4850 |
| 4851 | cctttttcaa | tattattgaa | gcatttatca | gggttattgt | ctcatgagcg | 4900 |
| 4901 | gatacatatt | tgaatgtatt | tagaaaaata | aacaaatagg | ggttccgcgc | 4950 |
| 4951 | acatttcccc | gaaaagtgcc | acctgaaatt | gtaaacgtta | atattttgtt | 5000 |
| 5001 | aaaattcgcg | ttaaattttt | gttaaatcag | ctcatttttt | aaccaatagg | 5050 |
| 5051 | ccgaaatcgg | caaaatccct | tataaatcaa | aagaatagac | cgagataggg | 5100 |
| 5101 | ttgagtgttg | ttccagtttg | gaacaagagt | ccactattaa | agaacgtgga | 5150 |
| 5151 | ctccaacgtc | aaagggcgaa | aaaccgtcta | tcagggcgat | ggcccactac | 5200 |
| 5201 | gtgaaccatc | accctaatca | agttttttgg | ggtcgaggtg | ccgtaaagca | 5250 |
| 5251 | ctaaatcgga | accctaaagg | gagcccccga | tttagagctt | gacggggaaa | 5300 |
| 5301 | gccggcgaac | gtggcgagaa | aggaagggaa | gaaagcgaaa | ggagcgggcg | 5350 |
| 5351 | ctagggcgct | ggcaagtgta | gcggtcacgc | tgcgcgtaac | caccacaccc | 5400 |
| 5401 | gccgcgctta | atgcgccgct | acagggcgcg | tcccattcgc | ca | 5442 |
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文献和实验调节因子的辅助报告基因实验中。一般, 我们建议在将要应用的目标细胞中验证一个特定辅助报告基因的性能。 PGL4.29 amp PGL4.26 amp PGL4.51 amp PGL4.75 amp 5)、原核表达载体(Novagen): pGEX4T-1 pGEX4T1 N端带GST在细菌内表达蛋白的载体 pGEX4T-3 pGEX4T3 N端带GST在细菌内表达蛋白的载体 pGEX6P-1 pGEX6P1 N端带GST在细菌内表达蛋白的载体 pET21b
b PET15b PET16b PET17b PET19b PET20b PET21b PET22b PET23a PET24a PET25
【资源】提供基因合成,载体构建,定点突变等服务;同时出售各种克隆载体,价格实惠!
) PCDNA4-TO Amp+ 500元(2-4ug) PET21b Amp+ 300元(2-4ug) PCDNA4-TO-mys-HisA Amp+ 500元(2-4ug) PET21d Amp+ 300元(2-4ug)
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