Anti-CaVγ3

Anti-Ca_Vγ3

Cacng3, TARP gamma-3, Transmembrane AMPAR regulatory protein gamma-3
Cat #: ACC-1113
Sizes: 25 µl, 50 µl, 0.2 ml
Source: Rabbit
Type: Polyclonal
Applications: WB
May also work in: IC, IFC, IH, IP
Reactivity: H, M, R

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
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Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Ca_Vγ3. Anti-Ca_Vγ3 antibody (#ACC-113) can be used in western blot analysis. It has been designed to recognize Ca_Vγ3 from rat, mouse and human samples.

• Applications
• Specifications
• Scientific Background
• Related Products

Western blot

Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and human neuroblastoma (SH-SY5Y) cells (lanes 3 and 6):
1-3. Anti-Ca_Vγ3 antibody (#ACC-113), (1:1000).
4-6. Anti-Ca_Vγ3 antibody, preincubated with the control peptide antigen.
Immunogen
Peptide (C)RSHSELLKKSTFAR, corresponding to amino acid residues 210-223 of rat Ca_Vγ3 (Accession Q8VHX0). Intracellular, C-terminus.

HomologyMouse - identical, human - 12/14 amino acid residues identical.
PurityAffinity purified on immobilized antigen.
FormulationLyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.
Standard quality control of each lotWestern blot analysis.
Peptide confirmationConfirmed by amino acid analysis and mass spectrometry.
Storage before reconstitutionThe antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution0.8 mg/ml.
Storage after reconstitutionThe reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Control antigen storage before reconstitutionLyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution100 µl double distilled water (DDW).
Control antigen storage after reconstitution-20ºC.
Preadsorption Control1 µg peptide per 1 µg antibody.
Scientific background

Voltage-gated Ca²⁺ (Ca_V) channels are ubiquitously expressed and function as Ca²⁺ conducting pores in the plasma membrane¹. Based on their electrophysiological and pharmacological properties, Ca²⁺ channels have traditionally been classified into L, T, N, P/Q, and R types².

L-type calcium channels are heteromultimers composed of four independently encoded proteins, the pore-forming α1 subunit, which triggers Ca²⁺ flow across the membrane, and the subunits α2δ, γ, and β³.

The γ subunit is an integral membrane protein. The γ family consists of at least 8 members, which share a number of common structural features. Each member is predicted to possess four transmembrane domains, with intracellular N- and C-termini. The first extracellular loop contains a highly conserved N-glycosylation site and a pair of conserved cysteine residues⁵. Ca_Vγ subunits inhibit Ca_V channel activity and modulate its activation and inactivation kinetics. Ca_Vγ subunits have little effect on Ca_V channel trafficking⁴. Ca_Vγ3 mRNAs are only detectable in mouse brain⁶.

References

1. Catterall, W.A. (2000) Annu. Rev. Cell. Dev. Biol. 16, 521.
2. Qin, N. et al. (2002) Mol. Pharmacol. 62, 485.
3. De Jongh, K.S. et al. (1990) J. Biol. Chem. 265, 14738.
4. Arikkath, J. et al. (2003) Curr. Opin. Neurobiol. 13, 298.
5. Lacinova, L. et al. (2005) Gen. Physiol. Biophys. Suppl.1, 1.
6. Klugbauer N. et al. (2000) FEBS Lett. 470, 189.