Anti-CaVγ8

Anti-Ca_Vγ8

TARP gamma-8, Voltage-dependent calcium channel gamma-8 subunit, Cacng8
Cat #: ACC-125
Sizes: 25 µl, 50 µl, 0.2 ml
Source: Rabbit
Type: Polyclonal
Applications: IH, WB
May also work in: IC, IFC, IP
Reactivity: M, R
May also work in: H

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
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Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Ca_Vγ8. Anti-Ca_Vγ8 antibody (#ACC-125) can be used in western blot and immunohistochemistry applications. It has been designed to recognize Ca_Vγ8 from rat, mouse and human samples.

• Applications
• Specifications
• Scientific Background
• Related Products

Western blot

Western blot analysis of rat testis (lanes 1 and 4), rat brain (lanes 2 and 5) and mouse brain (lanes 3 and 6) lysates:
1-3. Anti-Ca_Vγ8 antibody (#ACC-125), (1:400).
4-6. Anti-Ca_Vγ8 antibody, preincubated with the control peptide antigen.
Immunohistochemistry

Expression of Ca_Vγ8 in rat hippocampus
Immunohistochemical staining of rat hippocampus using Anti-Ca_Vγ8 antibody (#ACC-125). A. Ca_Vγ8 staining (green) appears in neuronal soma (horizontal arrows) and in the neuronal processes (diagonal arrows). B. Nuclear staining using DAPI as the counterstain (blue). C. Merged image of A and B.
Immunogen
Peptide (C)ESLKRWNEERGLW(S)EK, corresponding to amino acid residues 2-17 of rat Ca_Vγ8 (Accession Q8VHW5). Intracellular, N-terminus.

HomologyRat, mouse, human - 15/16 amino acid residues identical.
PurityAffinity purified on immobilized antigen.
FormulationLyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.
Standard quality control of each lotWestern blot analysis.
Peptide confirmationConfirmed by amino acid analysis and mass spectrometry.
Storage before reconstitutionThe antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution0.85 mg/ml.
Storage after reconstitutionThe reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Control antigen storage before reconstitutionLyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution100 µl double distilled water (DDW).
Control antigen storage after reconstitution-20ºC.
Preadsorption Control1 µg peptide per 1 µg antibody.
Scientific background

Transmembrane AMPA receptor regulatory proteins (TARPs) serve as auxiliary subunits of AMPA receptors that regulate functional aspects of these receptors such as: fast excitatory synaptic transmission, surface trafficking, enhancing synaptic clustering and increasing glutamate affinity. Generally, TARPs are responsible for regulating expression, channel properties and localization of AMPA receptors in the brain¹.

TARPs are non-pore-forming integral membrane proteins with four transmembrane domains that are widely expressed in the CNS. 

The TARP family is divided in Type I and type II subfamily. Type I TARPs comprise four calcium channel γ subunits: γ2, γ3, γ4, and γ-8, also known as Cacng2, 3, 4 and 8, respectively^2,3.

γ-8 TARP predominantly expressed in the telencephalon with abundant distribution on hippocampal excitatory synapses and extrasynaptic membranes. It is expressed in low levels in newborn and neonatal brain and can be found in higher levels in adult brain⁴. γ-8 plays an important role in increasing the number of synaptic and extrasynaptic AMPA receptors on dendrites and spines⁵. 

The C terminus of γ-8 contains 3 unique stretches of amino acids that are not found in any of the other TARPs. In addition, it contains PDZ-binding motifs². In heterologous cells, γ-8 prolong the current rise time in response to small amounts of glutamate and slow GluA1 receptor desensitization and deactivation³.

Several studies have shown that overexpression of TARP γ-8 increases the number of AMPARs in the plasma membrane. In γ-8 knockout mice, AMPA receptor expression is severely reduced from the synaptic and extrasynaptic membranes of dendrites and does not progress through the secretory pathway^5,6_.

References

1. Cho, C.H. et al. (2007) Neuron 55, 890.
2. Milstein, A.D. et al. (2009) Proc. Natl. Acad. Sci. U.S.A. 106, 11348.
3. Hamad, M.I. et al. (2014) Development 141, 1737.
4. Tomita, S. et al. (2003) J. Cell Biol. 161, 805.
5. Fukaya, M. et al. (2006) Eur. J. Neurosci. 24, 2177.
6. Kato, A.S. et al. (2010) Neuron 68,1082.