Anti-CaVγ4

Anti-Ca_Vγ4

Transmembrane AMPAR Regulatory Protein Gamma-4, TARP Gamma-4, CACNG4, Voltage-dependent calcium channel gamma-4 subunit, Neuronal voltage-gated calcium channel gamma-4 subunit
Cat #: ACC-114
Sizes: 25 µl, 50 µl, 0.2 ml
Source: Rabbit
Type: Polyclonal
Applications: WB
May also work in: IC, IH, IP
Reactivity: H, M, R

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
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Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Ca_Vγ4. Anti-Ca_Vγ4 antibody (#ACC-114) can be used in western blot analysis. It has been designed to recognize Ca_Vγ4 from rat, mouse and human samples.

• Applications
• Specifications
• Scientific Background
• Related Products

Western blot

Western blot analysis of rat brain (lanes 1 and 5), mouse brain (lanes 2 and 6), SH-SY5Y (lanes 3 and 7) and PC-12 (lanes 4 and 8) lysates:
1-4. Anti-Ca_vγ4 antibody (#ACC-114), (1:800).
5-8. Anti-Ca_vγ4 antibody, preincubated with the control peptide antigen.
Immunogen
Peptide (C)EKNKELRFKTKRE, corresponding to amino acid residues 208-220 of rat Ca_vγ4 (Accession Q8VHW9). Intracellular, C-terminus.

HomologyMouse, human - identical.
PurityAffinity purified on immobilized antigen.
FormulationLyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.
Standard quality control of each lotWestern blot analysis.
Peptide confirmationConfirmed by amino acid analysis and mass spectrometry.
Storage before reconstitutionThe antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution0.8 mg/ml.
Storage after reconstitutionThe reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Control antigen storage before reconstitutionLyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution100 µl double distilled water (DDW).
Control antigen storage after reconstitution-20ºC.
Preadsorption Control1 µg peptide per 1 µg antibody.
Scientific background

Voltage dependent Ca²⁺ channels (Ca_V channels) are pivotal players in many physiological roles such as secretion, contraction migration and excitation¹.

They are composed of several subunits; α1, β, α2δ and γ. Ca_V channels were originally divided into six physiological types: L, N, P, Q, R, and T type².

L-type calcium channels are composed of five subunits. Ca_vγ4, represents one of these subunits, and is one of several γ subunit proteins. It is an integral membrane protein that is thought to stabilize the Ca²⁺ channel in an inactive (closed) state. The encoding gene is a member of the neuronal Ca²⁺ channel γ subunit gene subfamily of the PMP-22/EMP/MP20 family and is located in a cluster with two similar gamma subunit-encoding genes³.

Ca_vγ4, along with Ca_vγ2, Ca_vγ3 and Ca_vγ8 have been associated with AMPA receptors and have been shown to modulate the trafficking of receptors to the plasma membrane as well as electrophysiological key properties⁴.

References

1. Tsunemi, T. et al. (2002) J. Biol. Chem. 277, 7214.
2. Scheuber, A. et al. (2004) J. Neurosci. 24, 10402.
3. Burgess, D.L. et al. (1999) Genome Res. 9, 1204.
4. Korber, C. et al. (2007) J. Neurosci. 27, 8442.