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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
见官方网站
- 亚型:
见官方网站
- 形态:
冻干粉或液体
- 保存条件:
-20ºC
- 克隆性:
多克隆
- 标记物:
见官方网站
- 适应物种:
见官方网站
- 保质期:
1年
- 抗原来源:
见官方网站
- 目录编号:
HYB 249-028
- 级别:
见官方网站
- 库存:
30
- 供应商:
上海泽叶生物科技有限公司
- 宿主:
见官方网站
- 应用范围:
ELISA
- 浓度:
1 mg/ml
- 靶点:
见官方网站
- 抗体英文名:
Anti-Vitamin D Binding Protein (VDBP)
- 抗体名:
Anti-Vitamin D Binding Protein (VDBP)
- 规格:
200ug
Anti-Vitamin D Binding Protein (VDBP)
Bioporto是一家体外诊断公司,专业为临床和科研提供一系列诊断试剂盒和抗体。其领先的产品组合包括了用于急性肾功能损伤诊断用的NGAL以及其它疾病检测用抗体。官方网站:www.bioporto.com
- Cat. No.
- HYB 249-028
- Product name
- Anti-Vitamin D Binding Protein (VDBP)
- Description
-
Mouse monoclonal antibody
- Specificity
-
HYB 249-02 is specific for human Vitamin D Binding Protein (VDBP)
- Immunogen
-
VDCP isolated from human plasma
- Application
- ELISA, WB
- Subclass
-
IgG1/k
- Gene ID
- 2638
- Clone number
- 5E8
- Epitope
-
Epitope specificity differs from that of HYB 249-01, HYB 249-05 and HYB 249-10.
- Content
-
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
- Purification
- Protein-A purified
- Form
- Liquid
- Solvent
- 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
- Storage
- 4-8ºC without exposure to light. No precautions necessary during handling.
- Target
-
Vitamin-D binding protein (VDBP, DBP, Gc-globulin) is a plasma protein produced in the liver. VDBP has several physiologically important functions such as transporting vitamin D and its metabolites to target cells, binding and sequestering globular actin and binding fatty acids to functioning in the immune system. VDBP is furthermore part of the actin scavenging system, binding and removing monomeric actin from the blood stream. The molecular mass of VDBP is approximately 50 kDa and the concentration of VDBP in human plasma is app. 400 µg/ml.
- Application 1
-
ELISA
HYB 249-02 reacts strongly with VDBP. A strong reaction is also seen in ELISA with VDBP. In order to measure total VDBP a combination of HYB 249-05 as capture antibody and HYB 249-02B as biotinylated detection antibody can be used.

The calibration curve of a sandwich assay for total VDBP using HYB 249-05 as the capture antibody and HYB 249-02B as the biotinylated detection antibody. - Application 2
-
WB
In Western blotting after SDS-PAGE, HYB 249-02 reacts with VDBP in both reduced as well as unreduced forms
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文献和实验STAINING NONFILAMENTOUS ACTIN WITH VITAMIN-D BINDING PROTEIN
STAINING NONFILAMENTOUS ACTIN WITH VITAMIN-D BINDING PROTEIN Indirect Method Materials 1. Gc-globulin (vitamin-D binding protein; Calbiochem 345802), prepare 2 mg/ml stock in PBS solution A, clarify 25,000 rpm for 20 min in a 42
Steroid hormone receptors activate and repress gene transcription through an integrated series of protein-protein interactions. One principle contact is the interaction of the nuclear receptors with one another to form both homodimeric
are not as important, as you are looking for a relatively small number of differences and so can devote more time to the identification of each protein.2D electrophoresis is performed in two steps(1) Isoelectric focusing (IEF) (First dimension electrophoresis): is used
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