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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
见官方网站
- 亚型:
见官方网站
- 形态:
冻干粉或液体
- 保存条件:
-20ºC
- 克隆性:
多克隆
- 标记物:
见官方网站
- 适应物种:
见官方网站
- 保质期:
1年
- 抗原来源:
见官方网站
- 目录编号:
ABS 005-192
- 级别:
见官方网站
- 库存:
30
- 供应商:
上海泽叶生物科技有限公司
- 宿主:
Human
- 应用范围:
ELISA, WB
- 浓度:
1 mg/ml
- 靶点:
见官方网站
- 抗体英文名:
Anti-L-Ficolin (human)
- 抗体名:
Anti-L-Ficolin (human)
- 规格:
200ug
Anti-L-Ficolin (human)
Bioporto是一家体外诊断公司,专业为临床和科研提供一系列诊断试剂盒和抗体。其领先的产品组合包括了用于急性肾功能损伤诊断用的NGAL以及其它疾病检测用抗体。官方网站:www.bioporto.com
- Cat. No.
- ABS 005-192
- Product name
- Anti-L-Ficolin (human)
- Description
-
Mouse monoclonal antibody
- Specificity
-
ABS 005-19 reacts specifically with L-Ficolin. No cross reactivity is seen with M-Ficolin (Ficolin-1) and H-Ficolin (Ficolin-3).
- Immunogen
-
Purified procaryot expressed recombinant human L-Ficolin and full-length L-Ficolin (Ficolin-2) protein
- Application
- ELISA, WB
- Species reactivity (positive)
- Human
- Species reactivity (negative)
- Not determined
- Subclass
-
IgG2a/k
- Gene ID
- 2220
- Clone number
- 19
- Epitope
-
Not determined
- Content
-
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
- Purification
- Protein-A purified
- Form
- Liquid
- Solvent
- 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
- Storage
- 4-8ºC without exposure to light. No precautions necessary during handling.
- Target
-
L-Ficolin (Ficolin-2) is an innate immunity pattern recognition molecule found in human serum. L-Ficolin binds to distinct pathogen-associated molecular patterns (PAMP), such as carbohydrates (GlcNAc), lipoteichoic acid and acetylated groups thereby facilitating phagocytosis and activation of complement through the lectin route using the same serine proteases as mannose-binding lectin (MBL) named MASP ((MBL)-associated serine protease) (1).
- Application 1
-
ELISA
ABS 005-19 is especially well suited as detecting antibody in a sandwich ELISA in combination with ABS 005-16 (1). Furthermore, in ELISA, ABS 005-19 reacts with recombinant L-Ficolin coated directly into the microtiter well. - Application 2
-
WB
ABS 005-19 can be used in Western blotting (1, 2, 3)
- Application 3
-
IP
ABS 005-19 can be used in immunoprecipitation (4).
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文献和实验Purification, Measurement of Concentration, and Functional Complement Assay of Human Ficolins
Ficolins constitute a group of lectins involved in innate immunity. L-Ficolin, H-ficolin, and M-ficolin are present in human serum. The human ficolins differ in carbohydrate-binding specificity, but they have in common the ability
Human PBMCs can be activated in vitro by soluble anti-human CD3 antibodies. We have performed titration studies with these antibodies and established the following protocol for stimulation of PBMC. Materials 1X sterile PBS Anti
人 Neuritin 酶联免疫分析(ELISA) 试剂盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆及相关液体样本 中Neuritin 的含量。 实验原理: 本试剂盒应用双抗体夹心法测定标本中人 Neuritin 水平。用纯化的 Neuritin 抗体 包被微孔板,制成固相抗体,往包被单抗的微孔中加入 Neuritin ,再与 HRP 标记的 Neuritin 抗体结合,形成抗体
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