Anti-Complement component C5a/

Anti-Complement component C5a/C5a(desArg) anaphylatoxin (human)

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Cat. No.

GAU 025-05

Product name

Anti-Complement component C5a/C5a(desArg) anaphylatoxin (human)

Description

Mouse monoclonal antibody

Specificity

GAU 025-05 reacts with human C5a/C5a(desArg)

Immunogen

Purified human C5a 

Application

ELISA, WB

Species reactivity (positive)

Human, baboon

Species reactivity (negative)

Not determined

Subclass

IgG1/k

Gene ID

725

Clone number

C17/5

Epitope

GAU 025-05 binds to a neoepitope exposed on C5a/C5a(desArg) (1).

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein-A purified

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Complement C5a is generated in the circulation by the activation of complement. It is a so-called anaphylatoxin of 74 amino-acid residues generated by the action of C5 convertase, which cleaves C5 into C5a and C5b. It is rapidly inactivated by serum carboxypeptidase B, which removes the C-terminal arginine residue generating C5a(desArg) (1).

Application 1

ELISA
GAU 025-05 reacts well with C5a/C5a(desArg). A strong reaction is seen in ELISA with recombinant C5a coated onto the microtiter well. (1)

Application 2

WB
GAU 025-05 can be used in Western blotting (1).