Anti-Ghrelin (human)

Anti-Ghrelin (human)

Bioporto是一家体外诊断公司，专业为临床和科研提供一系列诊断试剂盒和抗体。其领先的产品组合包括了用于急性肾功能损伤诊断用的NGAL以及其它疾病检测用抗体。
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Cat. No.

ABS 050-45

Product name

Anti-Ghrelin (human)

Description

Mouse monoclonal antibody

Specificity

ABS 050-45 binds human octanoylated ghrelin and human des-acyl ghrelin.
 

Immunogen

Synthetic human Ser3-octanoyl ghrelin-28

Application

ELISA, WB

Species reactivity (positive)

Human, rat (5%)

Species reactivity (negative)

Not determined

Subclass

IgG1/l

Gene ID

51738

Clone number

45

Epitope

Not determined. Probably includes residues 11 or 12 to account for the lower cross-reactivity with rat ghrelin.

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein-A purified

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Ghrelin is an orexigenic (food intake stimulating) 27 or 28 amino-acid gut-brain peptide hormone produced by endocrine cells in the stomach, pancreatic islets and certain hypothalamic neurons. Two forms are produced by alternative mRNA splicing; Gln13 or 14 is missing from the second form. "Active" ghrelin is acylated (octanoylated) at Ser3; this form stimulates growth hormone secretion via GHS receptors and increases appetite. The des-acyl form does not have these effects but may have other actions. Plasma responses of total ghrelin to food may reflect those of active ghrelin.

Application 1

ELISA
ABS 050-45 binds human ghrelin in solution.
ABS 050-45 (as capture antibody) forms a pair with ABS 052-121 as biotinylated detection antibody for measuring total human ghrelin by sandwich ELISA.
 

The calibration curve of a sandwich assay for Ghrelin using ABS 050-45 as the capture antibody and ABS 052-121B as the biotinylated detection antibody.

Application 2

WB
ABS 050-45 can be used in Western blotting.